Super Strong Bonding at the interface between ETL and Perovskite for Robust Flexible Optoelectronic Devices.

Organic-inorganic hybrid perovskites have demonstrated great potential for flexible optoelectronic devices due to their superior optoelectronic properties and structural flexibility. However, mechanical deformation-induced cracks at the buried interface and delamination from the substrate severely constrain the optoelectronic performance and device lifespan. Here, we design a two-site bonding strategy aiming to reinforce the mechanical stability of the SnO2/perovskite interface and perovskite layer using a multifunctional organic salt, 4-(trifluoromethoxy)phenylhydrazine hydrochloride (TPH).

Combating COVID-19 as a designated hospital: Experience from Shanghai, China.

Many large international cities, such as Shanghai, are facing the threat of more imported cases of COVID-19 because of the frequent flow of people and objects at home and abroad. In the face of the complex and changing disease status of the international community, dealing with this disease effectively is a great challenge to the city's existing public health emergency response capacity and also a major test of designated COVID-19 hospitals. Here, we share our experience as a designated COVID-19 hospital in Shanghai, China in terms of ) A Professional Multi-disciplinary Team, ) Personalized Treatment Plans for Patients in Severe or Critically Ill Condition, ) Well-organized Classification of Patients, ) Establishment of Transitional Wards, ) Nosocomial Infection Prevention and Control, and ) Identification and Reporting of the Asymptomatic in the hopes that these approaches can serve as a reference for healthcare providers and medical staff who are fighting the pandemic.

Localized Chemical Remodeling for Live Cell Imaging of Protein-Specific Glycoform.

Live cell imaging of protein-specific glycoforms is important for the elucidation of glycosylation mechanisms and identification of disease states. The currently used metabolic oligosaccharide engineering (MOE) technology permits routinely global chemical remodeling (GCM) for carbohydrate site of interest, but can exert unnecessary whole-cell scale perturbation and generate unpredictable metabolic efficiency issue. A localized chemical remodeling (LCM) strategy for efficient and reliable access to protein-specific glycoform information is reported.

A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells.

A lysosome-accessing nanoprobe is designed for recognition of lysosomal neuraminidases (Lyso-Neus), which can cleave the 4-methylumbelliferone moieties of the substrate from the nanoprobe, and lead to the escape of the moieties from acidic lysosomes into the neutral cytosol assisted by cationic poly(ethyleneimine) to light up the pH-responsive fluorescence for visual detection and dynamic tracking of Lyso-Neu activity in living cells.

A cascade amplification approach for visualization of telomerase activity in living cells.

An intracellular cascade amplification strategy for ultrasensitive "off-on" imaging of telomerase activity in living cells was designed. The method was based on fabrication of a dual function module-encapsulated liposome nanoprobe, which consisted of a telomerase-targeting responder-transmitter DNA complex (HPT) module and a catalyzed hairpin assembly (CHA) signal amplification module. Upon transfected into living cells, the released HPT could be specifically recognized and extended by telomerase, leading to the release of the transmitter DNA.