Duraplasty promotes functional recovery by alleviating intraspinal pressure and edema following severe spinal cord compression injury in rabbits: experimental studies.

Background: After acute traumatic spinal cord injury (tSCI), various surgical strategies have been developed to alleviate elevated intraspinal pressure (ISP) and secondary injury.

Purpose: Our study aimed to investigate the impacts of duraplasty and laminectomy on edema progression, perfusion and functional outcomes after severe balloon compression SCI.

Study Design: In vivo animal study.

Inhibiting DNA methyltransferase DNMT3B confers protection against ferroptosis in nucleus pulposus and ameliorates intervertebral disc degeneration via upregulating SLC40A1.

Epigenetic changes are important considerations for degenerative diseases. DNA methylation regulates crucial genes by epigenetic mechanism, impacting cell function and fate. DNA presents hypermethylation in degenerated nucleus pulposus (NP) tissue, but its role in intervertebral disc degeneration (IVDD) remains elusive.

RARRES2 is involved in the "lock-and-key" interactions between osteosarcoma stem cells and tumor-associated macrophages.

Osteosarcoma (OS) is a type of tumor. Osteosarcoma stem cells (OSCs) are responsible for drug resistance, recurrence, and immunosuppression in OS. We aimed to determine the heterogeneity of OSCs and the immunosuppression mechanisms underlying the interactions between OSCs and tumor-associated macrophages (TAMs).

Decomposition and humification process of domestic biodegradable waste by black soldier fly (Hermetia illucens L.) larvae from the perspective of dissolved organic matter.

Black soldier fly larvae (Hermitia illucens L.) (BSFL) bioconversion is a promising technology for domestic biodegradable waste (DBW) management and resource recovery. However, little is known about the DBW biodegradation during the BSFL bioconversion from the perspective of dissolved organic matter (DOM).

Role of ferroptosis-associated genes in ankylosing spondylitis and immune cell infiltration.

Ankylosing spondylitis (AS) is a chronic progressive autoimmune disease with insidious onset, high rates of disability among patients, unknown pathogenesis, and no effective treatment. Ferroptosis is a novel type of regulated cell death that is associated with various cancers and diseases. However, its relation to AS is not clear.

Targeting Ferroptosis Holds Potential for Intervertebral Disc Degeneration Therapy.

Intervertebral disc degeneration (IVDD) is a common pathological condition responsible for lower back pain, which can significantly increase economic and social burdens. Although considerable efforts have been made to identify potential mechanisms of disc degeneration, the treatment of IVDD is not satisfactory. Ferroptosis, a recently reported form of regulated cell death (RCD), is characterized by iron-dependent lipid peroxidation and has been demonstrated to be responsible for a variety of degenerative diseases.

Theaflavin-3,3'-Digallate Plays a ROS-Mediated Dual Role in Ferroptosis and Apoptosis via the MAPK Pathway in Human Osteosarcoma Cell Lines and Xenografts.

Globally, osteosarcoma (OS) is the most prevalent form of primary bone cancer in children and adolescents. Traditional neoadjuvant chemotherapy regimens have reached a bottleneck; thus, OS survivors have unsatisfactory outcomes. Theaflavin-3,3'-digallate (TF3) exhibits potent anticancer properties against many human cancers.

Elevated intraspinal pressure drives edema progression after acute compression spinal cord injury in rabbits.

Elevated intraspinal pressure (ISP) following traumatic spinal cord injury (tSCI) can be an important factor for secondary SCI that may result in greater tissue damage and functional deficits. Our present study aimed to investigate the dynamic changes in ISP after different degrees of acute compression SCI in rabbits with closed canals and explore its influence on spinal cord pathophysiology. Closed balloon compression injuries were induced with different inflated volumes (40 μl, 50 μl or no inflation) at the T7/8 level in rabbits.

Black soldier fly larvae vermicompost alters soil biochemistry and bacterial community composition.

Black soldier fly larvae (Hermetia illucens L. BSFL) bioconversion is a promising biotechnology for food waste recycling, yet little is known about how BSFL vermicompost affects soil health in terms of element availability and related microbial response. In this work, a field soil experiment for luffa (Luffa cylindrica (L.

Insights on dissolved organic matter and bacterial community succession during secondary composting in residue after black soldier fly larvae (Hermetia illucens L.) bioconversion for food waste treatment.

Black soldier fly larvae (Hermetia illucens L. BSFL) bioconversion is a promising biotechnology for food waste treatment. However, the separated residues still do not meet criteria for use as land application biofertilizers.

PM-bound elements in Hebei Province, China: Pollution levels, source apportionment and health risks.

Particle-bound elements have attracted increasing attentions due to their health effects and atmospheric catalytic reactivity. However, elements in atmospheric fine particulate matter (PM) have not been well investigated even in some highly polluted area. In this study, 22 elements in PM were measured by a multi-metal monitor in ten prefecture-level and county-level cities in Hebei province, one of the most polluted provinces in China, during the heating and non-heating seasons.

Correction: Cadmium-Induced Oxidative Stress and Apoptotic Changes in the Testis of Freshwater Crab, Sinopotamon henanense.

[This corrects the article DOI: 10.1371/journal.pone.

Ruthenium(II) complex-based fluorescent sensor for peroxynitrite.

We have developed a new ruthenium complex, Ru(bpy)(2)[4-(2,2'-bipyridin-4-yloxy)phenol]Cl(2) (RuL), as a fluorescent sensor to detect peroxynitrite (ONOO(-)). The results showed that the addition of ONOO(-) to the aqueous solution of RuL would result in distinct fluorescence quenching at 600nm. RuL exhibits a good selectivity for ONOO(-) over other reactive oxygen species (ROS) and reactive nitrite species (RNS), and the reaction time is less than 1.

Synthesis and properties of fluorescence dyes: tetracyclic pyrazolo[3,4-b]pyridine-based coumarin chromophores with intramolecular charge transfer character.

Two series of new tetracyclic pyrazolo[3,4-b]pyridine-based coumarin chromophores were synthesized through a facile reaction between 3-aldehyde-7-diethylaminocoumarin (5) or 3-acetyl-7-diethylaminocoumarin (6) and 5-aminopyrazole derivatives (7) in a one-pot procedure. Different condensed products were obtained from compounds 5 and 6, and the potential reaction mechanism was studied using the reaction of 5 with 5-amino-1-phenylpyrazole (7a). The molecular structures were characterized by NMR and HRMS and confirmed by X-ray diffraction.

Cadmium-induced oxidative stress and apoptotic changes in the testis of freshwater crab, Sinopotamon henanense.

Cadmium (Cd), one of the most toxic environmental and industrial pollutants, is known to exert gonadotoxic and spermiotoxic effects. In the present study, we examined the toxic effect of Cd on the testis of freshwater crab, Sinopotamon henanense. Crabs were exposed to different Cd concentrations (from 0 to 116.

Reversible fluorescent probe for highly selective and sensitive detection of mercapto biomolecules.

A coumarin-derived complex, Hg(2)L(2), was reported as a highly sensitive and selective probe for the detection of mercapto biomolecules in aqueous solution. The addition of Cys to a 99% aqueous solution of Hg(2)L(2) resulted in rapid and remarkable fluorescence OFF-ON (emission at 525 nm) due to the ligand-exchange reaction of Cys with L coordinated to Hg(2+). The increased fluorescence can be completely quenched by Hg(2+) and recovered again by the subsequent addition of Cys.

Enzyme sensing based on a controllable oxidation reaction.

We developed a new method for glutathione reductase (GR) enzyme sensing via a metal-controlled spontaneous oxidation reaction. A new complex HgL, composed of 3-benzothiazoliny-7-N,N-diethylaminocoumarin (L) and HgCl2, was used as an example for illustration. It was found that ligand L was released from complex HgL by a ligand exchange process in the presence of GSH, which was enzymatic reduced from GSSG in the presence of NADH.

Colorimetric test kit for Cu2+ detection.

A coumarin-based colorimetric chemosensor 1 was designed and synthesized. It exhibits good sensitivity and selectivity for the copper cation over other cations such as Zn(2+), Cd(2+), Pb(2+), Co(2+), Fe(2+), Ni(2+), Ag(+), and alkali and alkaline earth metal cations both in aqueous solution and on paper-made test kits. The change in color is very easily observed by the naked eye in the presence of Cu(2+) cation, whereas other metal cations do not induce such a change.