Electrochemical detection of the neurotransmitter glutamate and the effect of the psychotropic drug riluzole on its oxidation response.

Glutamate is the main excitatory neurotransmitter in the brain and plays a leading role in degenerative diseases, such as motor neuron diseases. Riluzole is a glutamate regulator and a therapeutic drug for motor neuron diseases. In this work, the interaction between glutamate and riluzole was studied using cyclic voltammetry and square-wave voltammetry at a glassy carbon electrode (GCE).

The Role of KDM2A and H3K36me2 Demethylation in Modulating MAPK Signaling During Neurodevelopment.

Intellectual disability (ID) is a condition characterized by cognitive impairment and difficulties in adaptive functioning. In our research, we identified two de novo mutations (c.955C>T and c.

Effects of olanzapine and lithium carbonate antipsychotic agents on dopamine oxidation.

Olanzapine (OLZ) and lithium carbonate (LiCO) are the main drugs for treating mental disorders related to dopamine (DA). A highly conductive carbon paper sensing electrode is used to investigate the effects of OLZ and LiCO on DA oxidation due to its amplification of oxidation peak currents. Different chemical properties of drugs have different effects on DA oxidation.

Recent strategies for electrochemical sensing detection of miRNAs in lung cancer.

MicroRNAs (miRNAs) associated with lung cancer are diversifying. MiR-21, Let-7, and miR-141 are common diagnostic targets. Some new lung cancer miRNAs, such as miR-25, miR-145, and miR-126, have received increasing attention.

HoO-TiO Nanobelts Electrode for Highly Selective and Sensitive Detection of Cancer miRNAs.

The design and engineering of effective electrode materials is critical in the development of electrochemical sensors. In the present study, HoO-TiO nanobelts were synthesized by an alkaline hydrothermal process. The structure and morphology were investigated by X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) measurements.

Protein detection based on rolling circle amplification sensors.

Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection.

Electrochemical sensing of pancreatic cancer miR-1290 based on yeast-templated mesoporous TiO modified electrodes.

Electrochemical sensing is an effective, low-cost technology for cancer detection. In this study, mesoporous TiO was prepared via biomimetic synthesis based on yeast cell templates, and used to prepare a modified electrode for the sensitive detection of pancreatic cancer miR-1290. The structure and the morphology of the TiO were characterized by X-ray diffraction (XRD), N adsorption-desorption isotherm (NADI), Atomic force microscopy (AFM), and electron probe microanalysis (EPMA).

Mini-Disk Capillary Array Coupling with LAMP for Visual Detection of Multiple Nucleic Acids using Genetically Modified Organism Analysis as an Example.

Convenient, portable, and low-cost multiplex nucleic acid testing (NAT) systems are the trends in the fields of food safety, environmental microorganisms, molecular diagnosis, etc. In this study, we developed a novel system for visual monitoring of multiple nucleic acids combining a mini-disk capillary array (diameter = 17 mm, embedded with 6-10 capillaries), visual loop-mediated isothermal amplification (LAMP), and quick DNA extraction called mDC-LAMP. The performance and applicability of mDC-LAMP in testing multiple nucleic acids were evaluated and verified employing genetically modified contents analysis as an example.

Analysis of differential expression profile of miRNA in peripheral blood of patients with lung cancer.

Purpose: To identify potential molecular targets for lung cancer intervention and diagnosis, we analyzed the differential miRNA expression of peripheral blood between lung cancer patients and healthy controls.

Methods: Three pairs of cases' and controls' peripheral blood samples were evaluated for miRNA expression by microarray. 12 miRNAs were selected for RT-PCR validation and target genes prediction.

Shikonin, vitamin K and vitamin K inhibit multiple glycolytic enzymes in MCF-7 cells.

Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin K and vitamin K, have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity.

Au/TiO₂ nanobelt heterostructures for the detection of cancer cells and anticancer drug activity by potential sensing.

Cancer is a cell dysfunction disease. The detection of cancer cells is extremely important for early diagnosis and clinical treatments. At present, the pretreatment for the detection of cancer cells is costly, complicated and time-consuming.

Fabricating high performance lithium-ion batteries using bionanotechnology.

Designing, fabricating, and integrating nanomaterials are key to transferring nanoscale science into applicable nanotechnology. Many nanomaterials including amorphous and crystal structures are synthesized via biomineralization in biological systems. Amongst various techniques, bionanotechnology is an effective strategy to manufacture a variety of sophisticated inorganic nanomaterials with precise control over their chemical composition, crystal structure, and shape by means of genetic engineering and natural bioassemblies.

Enhancement of electrochemical differentiation ability of nucleobases in phosphate buffer solution at pH 7.4.

The electrochemical behavior of nucleobases has been studied in 0.1 M phosphate buffer solution at pH 7.4, using a bare graphite electrode.

A strategy for sequencing based on rolling-circle amplification on a microarray.

The sequencing of DNA fragments has become an important facet of the study of genomes. Parallel genomic DNA fragments displayed on a microarray play a key role in producing a template in next generation DNA sequencing. Here, we developed a technique to display parallel genomic DNA fragments based on the reaction with Bst DNA polymerase on a microarray.

Nano-p-n junction heterostructure TiO nanobelts for the electrochemical detection of anticancer drug and biointeractions with cancer cells.

Nano-p-n junction heterostructures based on TiO nanobelts with enhanced (001) facets were produced by assembling p-type semiconductor NiO nanoparticles on n-type surface-coarsened TiO nanobelt surfaces. The heterostructures were then used as the sensing electrode for the electrochemical detection of anticancer drugs O-benzylguanine (OBG) and lung cancer cells. OBG exhibited an irreversible diffusion-controlled electrochemical process with an oxidation peak clearly identified at +0.

Electrocatalytic oxidation of nucleobases by TiO2 nanobelts.

Two kinds of TiO(2) nanobelts were prepared from commercial P-25 powders via an alkaline hydrothermal method with and without an acid etching process. The uncauterized nanobelts (TNs) exhibited a smooth surface, and mixed phases of anatase and TiO(2) (B), whereas the cauterized ones (CTNs) displayed a rough surface and a pure anatase structure. TNs and CTNs were then deposited onto a glassy carbon electrode (GCE) surface with a conductive adhesive (CA), and the resulting chemically modified electrodes exhibited electrocatalytic activities in the oxidation of nucleobases in a 0.

High-performance TiO(2) from Baker's yeast.

Based on the biomineralization assembly concept, a biomimetic approach has been developed to synthesize high-performance mesoporous TiO(2). The key step of this approach is to apply Baker's yeast cells as biotemplates for deriving the hierarchically ordered mesoporous anatase structure. The mechanism of formation of the yeast-TiO(2) is revealed by characterizing its morphology, microstructure, and chemical composition.

In vitro biomimetic construction of hydroxyapatite-porcine acellular dermal matrix composite scaffold for MC3T3-E1 preosteoblast culture.

The application of porous hydroxyapatite-collagen (HAp-Collagen) as a bone tissue engineering scaffold is hindered by two main problems: its high cost and low initial strength. As a native 3-dimenssional collagen framework, purified porcine acellular dermal matrix (PADM) has been successfully used as a skin tissue engineering scaffold. Here we report its application as a matrix for the preparation of HAp to produce a bone tissue scaffold through a biomimetic chemical process.

Enhancement of ethanol vapor sensing of TiO2 nanobelts by surface engineering.

TiO(2) nanobelts were prepared by a hydrothermal process, and the structures were manipulated by surface engineering, including surface coarsening by an acid-corrosion procedure and formation of Ag-TiO(2) heterostuctures on TiO(2) nanobelts surface by photoreduction. Their performance in the detection of ethanol vapor was then examined and compared by electrical conductivity measurements at varied temperatures. Of the sensors based on the four nanobelt samples (TiO(2) nanobelts, Ag-TiO(2) nanobelts, surface-coarsened TiO(2) nanobelts, and surface-coarsened Ag-TiO(2) nanobelts), they all displayed improved sensitivity, selectivity, and short response times for ethanol vapor detection, in comparison with sensors based on other oxide nanostructures.

Ag2O/TiO2 nanobelts heterostructure with enhanced ultraviolet and visible photocatalytic activity.

Ag(2)O/TiO(2) heterostructure with high photocatalytic activity both in ultraviolet and visible-light region was synthesized via a simple and practical coprecipitation method by using surface-modified TiO(2) nanobelts as substrate materials. The as-prepared heterostructure composite included Ag(2)O nanoparticles assembled uniformly on the rough surface of TiO(2) nanobelts. Comparing with pure TiO(2) nanobelts and Ag(2)O nanoparticles, the composite photocatalyst with a wide weight ratio between TiO(2) and Ag(2)O exhibited enhanced photocatalytic activity under ultraviolet and visible light irradiation in the decomposition of methyl orange (MO) aqueous solution.

Mesoporous zirconium phosphate from yeast biotemplate.

Mesoporous zirconium phosphate has attracted increasing interest due to its extraordinary functionalities. In particular, great progress has been made in the synthesis of mesoporous zirconium phosphate using traditional approaches. However, synthesis of mesoporous zirconium phosphate using yeast as biotemplate has not been well studied so far.

Ordered hierarchical mesoporous anatase TiO2 from yeast biotemplates.

A novel approach is proposed to prepare ordered hierarchical mesoporous TiO(2) by using yeast cells as the template via biomimetic mineralization. The structure and the mophology were characterized by atomic force microscopy (AFM), X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX), Raman spectra, high-resolution transmission electron microscopy (HRTEM), and N(2) adsorption-desorption isotherms (NADI). The bio-templated TiO(2) has a hierarchical pore structure mainly distributed at 4.