Investigating the epidemiological relevance of secretory otitis media and neighboring organ diseases through an Internet search.

Background: This study examined the epidemiological correlations between secretory otitis media (SOM) and diseases of neighboring organs. We measured changes in disease incidences during the 2020 COVID-19 pandemic using Internet big data spanning from 2011 to 2021.

Methods: This study used the Baidu Index (BI) to determine the search volume for the terms "secretory otitis media (SOM)", "tonsillitis", "pharyngolaryngitis", "adenoid hypertrophy (AH)", "nasopharyngeal carcinoma (NPC)", "nasal septum deviation (NSD)", "rhinosinusitis", "allergic rhinitis (AR)", and "gastroesophageal reflux disease (GERD)" in Mandarin from January 2011 to December 2021.

Developmental immunotoxicity and its potential gender differences of perinatal exposure to 4-nonylphenol on offspring rats: JAK-STAT signaling pathway involved.

The aim of our study was to explore the developmental immunotoxicity (DIT) and its potential gender differences of perinatal exposure to 4-nonylphenol (4-NP), which was significant for the risk assessment of 4-NP exposure to fetuses and infants. Wistar pregnant rats were given the National Institution of Health (NIH)- 31 modified feed containing 0, 10, 100 and 500 mg/kg 4-NP from the gestation day (GD) 6 to the postnatal day (PND) 21. At PND21, the offspring rats were randomly selected to detect developmental immunotoxicity related indicators.

Seven-gene signature on tumor microenvironment for predicting the prognosis of patients with pancreatic cancer.

Background: The aim of the present study was to construct a novel gene signature on the tumor microenvironment (TME) to predict the prognosis of patients with pancreatic ductal adenocarcinoma (PDAC).

Methods: We downloaded gene expression profiles and clinical information of PDAC from The Cancer Genome Atlas (TCGA) datasets, as well as Gene Expression Omnibus (GEO) datasets (GSE78229, GSE62452, and GSE28735). Differentially expressed genes were generated by comparing high versus low score groups of immune/stromal subgroups based on the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data algorithm.

[Endoscope-assisted transoral resection of parapharyngeal space tumors: a retrospective study].

To evaluate the feasibility and safety of the endoscope-assisted transoral approach for the parapharyngeal space（PPS） tumors. This retrospective study included 22 patients who were diagnosed with PPS tumors and treated with the endoscope-assisted transoral approach. The postoperative pathological diagnosis included 10 cases of schwannoma, 7 cases of pleomorphic adenoma, 1 case of paraganglioma, 1 case of branchial cleft cyst, and 3 cases of malignant tumors for a diagnostic purpose.

[The role of palatovaginal canal and sphenopalatine artery in the localization of vidian canal during vidian neurotomy].

To explore the role of palatovaginal canal and sphenopalatine artery in the localization of pterygoid canal during pterygoid neurotomy. 55 patients with or without nasal polyps were divided into two groups: group A（twenty-nine cases, anterior wall approach of sphenoid sinus） and group B（twenty-six cases, middle nasal meatus approach）. All patients underwent unilateral vidian neurectomy under nasal endoscope.

Downregulation of miR-29c-3p is associated with a poor prognosis in patients with laryngeal squamous cell carcinoma.

Background: Laryngeal squamous cell carcinoma (LSCC) is considered to be a common malignancy of the head and neck with poor prognosis for its late diagnosis, metastasis and recurrence. Growing evidence demonstrates that the dysregulation of miR-29c-3p (microRNA-29c-3p) plays an important role in various tumor processes. Our study investigates the expression of miR-29c-3p in LSCC and analyzes the correlation of its dysregulation with clinicopathologic parameters and prognosis.

[Effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells of guinea pigs].

Objective: To investigate the effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells (SGC) of guinea pigs.

Method: Twenty guinea pigs were randomly divided into cisplatin (n = 10) and control groups (n = 10). Cisplatin group were administrated with a dose of intraperitoneal injection of 16 mg/kg, while the control group were received intraperitoneal injection of normal saline as placebo.

The influence of house dust mite sublingual immunotherapy on the TSLP-OX40L signaling pathway in patients with allergic rhinitis.

Background: This study aimed to investigate the clinical efficacy of sublingual immunotherapy (SLIT) with house dust mite (HDM) extract and to examine T helper 2 (Th2)-type immune responses mediated by the thymic stromal lymphopoietin (TSLP-OX40L) signaling pathway in patients with moderate to severe allergic rhinitis (AR) after 12-month HDM SLIT.

Methods: Forty-six cases of HDM-sensitized patients with persistent AR in southern China were enrolled in this study. Clinical efficacy of SLIT was assessed by determining the individual nasal symptom score (INSS) and total nasal symptom score (TNSS) after 12-month HDM SLIT.

[Comparison of methods for isolating exosomes derived from laryngocarcinoma Hep-2 cells].

Objective: To provide a reference about choosing the methods of isolating exosomes derived from tumor cells including laryngocarcinima Hep-2 cells by comparing advantages and defects of two methods of isolation and extraction exosomes.

Method: Previously, laryngocarcinoma Hep-2 cells were cultivated massively, then the cells were processed with hot shock in 42 degrees C for 1 h. Sucrose density gradient centrifugation ultrafiltration (method 1): cells culture supernatant 90 ml was gathered, the supernatant was clarified through a 3/0.

[The effectiveness of endoscopic tragus cartilage-perichondrium myringoplasty in the treatment of large tympanic membrane perforations].

Objective: To evaluate effectiveness of endoscopic tragus cartilage-perichondrium in the treatment of large tympanic membrane perforations.

Method: Patients with large tympanic membrane perfortations who had underwent cartilage-perichondrium myringoplasty were retrospectively retrieved from our department. Those with a follow-up of equal to or greater than 12 months after surgery were included in the study.

[The influence of sublingual immunotherapy on Th2 type immunoreaction in patients with allergic rhinitis].

Objective: To explore the influence of sublingual immunotherapy on Th2 type immunoreaction in patients with allergic rhinitis within 12 months' treatment.

Method: Forty three patients with allergic rhinitis were followed-up within 12 months after receiving sublingual immunotherapy. The changes of clinic symptoms and immunoreaction were analyzed.

[Construction of the superantigen SEA transfected laryngocarcinoma cells].

Objective: To construct an eukaryotic expression vectors containing superantigen staphylococcal enterotoxin A (SEA) gene, and to identify its expression in laryngeal squamous carcinoma cells.

Method: SEA full-length gene fragment was obtained from ATCC13565 genome of the staphylococcus, referencing standard strains producing SEA. Coding sequence of SEA was artificially synthetized.

[Measurement of components of the phospholipid of the surfactant in irrigating fluid from the nasopharynx of patients with chronic sinusitis].

Objective: To investigate the effect of chronic sinusitis on components of the phospholipid of nasopharyngeal surfactant, and to study biochemical component of phospholipid of surface active substance.

Method: The concentrations of surfactant in nasopharyngeal irrigating fluid were implemented in normal controls and patients with chronic sinusitis. Components of phospholipid such as Phosphatidylserine, Phosphatidylethanolamine, Phosphatidylcholine and Sphingophospholipid were measured by the high-performance liquid chromatograph.

[Evaluation of tympanoplasty with reconstruction of the external canal after mastoidectomy for cholesteatoma].

Objective: To investigate the method to repair the deletion of external canal and opened mastoid space after mastoidectomy.

Method: One stage tympanoplasty with reconstruction of the external canal with temporal cortex was completed in 58 cases (58 ears) with cholesteatoma.

Result: During the follow-up ranging from one to three years, there was only one relapse.

[Evaluation on hemorrhage factors secondary to endoscopic sinus surgery with multiple stepwise regression analysis].

Objective: To investigate the factors relating to bleeding secondary to endoscopic sinus surgery.

Method: 193 patients suffered from nasal polyps, chronic sinusitis and underwent endoscopic sinus surgery between May 1995 and February 2001 were analyzed. These patients's state of operation affairs tallies with this study's selecting condition and their clinic data was complete.