Chromodomain helicase/ATPase DNA binding protein 1-like protein expression predicts poor prognosis in nasopharyngeal carcinoma.

Nasopharyngeal carcinoma (NPC) is a malignancy with a high metastatic ability. Recent studies have implicated the role of chromodomain helicase/ATPase DNA binding protein 1-like (CHD1L) gene as a novel oncogene; however, the functional role of CHD1L in NPC remains unknown. The aim of this study was to evaluate the clinical significance of CHD1L positivity in NPC.

Production of transgenic pigs mediated by pseudotyped lentivirus and sperm.

Sperm-mediated gene transfer can be a very efficient method to produce transgenic pigs, however, the results from different laboratories had not been widely repeated. Genomic integration of transgene by injection of pseudotyped lentivirus to the perivitelline space has been proved to be a reliable route to generate transgenic animals. To test whether transgene in the lentivirus can be delivered by sperm, we studied incubation of pseudotyped lentiviruses and sperm before insemination.

[Study on water processing conditions of Realgar].

Objective: To optimize the water processing (shui-fei) condition of Realgar.

Methods: The processing conditions were optimized by L9 (3)4 orthogonal design with three factors as water dose, process times and dryness temperature. The content of dissolved arsenic As(III) in Realgar was tested by High Performance Liquid Chromatography-hydride generation on-line coupled with atomic fluorescence spectrometry (HPLC-HG-AFS).