Assessing the in vivo efficacy of doxorubicin loaded hyaluronan nanoparticles.

Magnetic nanoparticles are attractive platforms for biomedical applications including diagnosis and treatment of diseases. We have shown previously that hyaluronan-coated superparamagnetic iron oxide nanoparticles (HA-SPIONs) enhanced the efficacy of the conjugated anticancer drug doxorubicin (DOX) in vitro against drug-sensitive and drug-resistant human ovarian cancer cells. In this manuscript, we report our findings on the efficacy of DOX loaded HA-SPIONs in vivo using subcutaneous and intraperitoneal SKOV-3 ovarian tumor models in nude mice.

Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs.

A simple synthetic route for the preparation of functional nanoscale graphene oxide (NGO), a novel nanocarrier for the loading and targeted delivery of anticancer drugs, is reported. The NGO is functionalized with sulfonic acid groups, which render it stable in physiological solution, followed by covalent binding of folic acid (FA) molecules to the NGO, thus allowing it to specifically target MCF-7 cells, human breast cancer cells with FA receptors. Furthermore, controlled loading of two anticancer drugs, doxorubicin (DOX) and camptothecin (CPT), onto the FA-conjugated NGO (FA-NGO) via pi-pi stacking and hydrophobic interactions is investigated.

Effect of surface charge and agglomerate degree of magnetic iron oxide nanoparticles on KB cellular uptake in vitro.

We synthesized three types of magnetic iron oxide nanoparticles (MNPs), which were meso-2,3-dimercaptosuccinic acid (DMSA) coated MNPs (DMSA@MNPs, 17.3+/-4.8 nm, negative charge), chitosan (CS) coated MNPs (CS@MNPs, 16.

The relationship between internalization of magnetic nanoparticles and changes of cellular optical scatter signal.

Carboxylmethyl starch sodium-coated magnetic nanoparticles (CMS@MNs) with average size of 10 nm were synthesized by chemical coprecipitation. Cellular iron content showed that CMS@MNs could be efficiently uptaken by human hepatoma cells. TEM image showed that clusters consisting of nanoparticles were enclosed within sub-micrometric endosomes and one cell contained several such endosomes.

Comparative study on radiosensitivity of various tumor cells and human normal liver cells.

Aim: To investigate the radiation response of various human tumor cells and normal liver cells.

Methods: Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with (60)Co gamma-rays. Cell survive was documented by a colony assay.