Relic DNA obscures bacterial diversity and interactions in ballast tank sediment.

The dark and anoxic environment of ballast tank sediment (BTS) harbors substantial amounts of relic DNA, yet its impact on microbial diversity estimates in BTS management remains poorly understood. This study employed propidium monoazide (PMA) treatment to eliminate relic DNA and used 16S amplicon high-throughput sequencing to characterize both total and viable bacteria. Our findings revealed that relic DNA is abundant in BTS.

Environmental RNA metabarcoding for ballast water microbial diversity: Minimizing false positives.

While maritime transport boosts global trade by shipping bulk goods, it raises concerns about the spread of harmful bacteria via ballast water. Moreover, the dark and cold environments of ballast tanks often harbor extracellular DNA from dead organisms, leading to false positives in traditional environmental DNA (eDNA) metabarcoding analyses. Here, we alternatively employed environmental RNA (eRNA) metabarcoding to assess its potential for reducing false positive in ballast water monitoring.

MHY1485 promotes adriamycin sensitivity in HepG2 cells by inhibiting autophagy.

MHY1485 is an mTOR activator that inhibits the autophagy process by inhibiting the fusion between autophagosomes and lysosomes. This study aimed to explore the role and mechanism of MHY1485 in hepatocellular carcinoma (HCC) and to provide an in-depth understanding of the mechanisms of autophagy regulation in relation to adriamycin (ADM) resistance, as well as the development of a molecularly targeted autophagy-modulating approach. Here, ADM was used to treat HepG2 cells and construct an ADM-resistant cell model.

An Improved Adam Optimization Algorithm Combining Adaptive Coefficients and Composite Gradients Based on Randomized Block Coordinate Descent.

An improved Adam optimization algorithm combining adaptive coefficients and composite gradients based on randomized block coordinate descent is proposed to address issues of the Adam algorithm such as slow convergence, the tendency to miss the global optimal solution, and the ineffectiveness of processing high-dimensional vectors. The adaptive coefficient is used to adjust the gradient deviation value and correct the search direction firstly. Then, the predicted gradient is introduced, and the current gradient and the first-order momentum are combined to form a composite gradient to improve the global optimization ability.

An improved two-stage label propagation algorithm based on LeaderRank.

To solve the problems of poor stability and low modularity () of community division results caused by the randomness of node selection and label update in the traditional label propagation algorithm, an improved two-stage label propagation algorithm based on LeaderRank was proposed in this study. In the first stage, the order of node updating was determined by the participation coefficient (PC). Then, a new similarity measure was defined to improve the label selection mechanism so as to solve the problem of label oscillation caused by multiple labels of the node with the most similarity to the node.

Hotel Review Classification Based on the Text Pretraining Heterogeneous Graph Neural Network Model.

With the amount of online information continuously growing, it becomes more and more important for online stores to recommend corresponding products precisely based on users' preferences. Reviews for various products can be of great help for the recommendation task. However, most recommendation platforms only classify positive and negative reviews based on sentiment analysis, without considering the actual demands of users, and it will reduce the effectiveness on classification task.

Color-dependent unidirectional scattering of a plasmonic heterodimer investigated by a dipole-dipole interference model.

In this paper, we employ an interference model of two separated electric dipoles to study the color-dependent unidirectional scattering of a plasmonic heterodimer consisting of a pair of gold and silver disks of the same size. The dipole moments in such a dipole-dipole interference model are numerically obtained by a multipole decomposition method. It shows that the power difference between the different scattering directions predicted by the dipole-dipole interference model agrees well with that calculated by the full wave simulation.

Cardiac VFM visualization and analysis based on YOLO deep learning model and modified 2D continuity equation.

In order to realize the visual analysis of cardiac fluid motion, according to the characteristics of cardiac flow field ultrasound image, a method for the cardiac Vector Flow Mapping (VFM) analysis and evaluation based on the You-Only-Look-Once (YOLO) deep learning model and the improved two-dimensional continuity equation is proposed in this paper. Firstly, based on the ultrasound Doppler data, the radial velocity values of the blood particles are obtained; due to the real-time VFM's high requirement on the computing speed, the YOLO deep learning model is combined with an improved block matching algorithm for the localization and tracking of myocardial wall, and then the azimuth velocity of myocardial wall speckles can be obtained; in addition, it is proposed in this paper to use a nonlinear weight function to fuse the radial velocity of the blood particles and azimuth velocity of myocardial wall speckles nonlinearly, and further the vortex streamline diagram in the cardiac flow field can be obtained. The results of the experiments on the evaluation of the Ultrasonic apical long-axis view show that the proposed method not only improves the accuracy of VFM, but also provides a new evaluation basis for cardiac function impairment.

The Relationship Between Magnetism and Microstructure of Ethylene Pyrolysis Furnace Tubes after a Long-term Service.

The magnetism and microstructure of Cr25Ni35Nb and Cr35Ni45Nb alloy tubes after 5 years of service were investigated in this paper. The saturation magnetization of the Cr25Ni35Nb alloy tube in the thickness direction is more than 20 emu/g, and the tube becomes ferromagnetic. The inner and outer walls of Cr35Ni45Nb alloy tubes also become ferromagnetic.

Identification of two lineages of host-associated eriophyoid mites predisposed to different levels of host diversification.

Herbivorous arthropods can diversify as a consequence of evolutionary changes in response to their plant hosts. Current patterns of host association of herbivores are likely to reflect a long evolutionary history of herbivore-plant co-evolution. Here, we used molecular phylogenetics to track the evolutionary history of host shifts and diversification of 66 eriophyoid mites (Acari, Eriophyoidea), and linked past patterns of evolutionary diversification to more recent patterns of divergence by tracking population genetic variation in 13 of the eriophyoid mite species feeding on different gymnosperm hosts.

Body mass index and exon 19 mutation as factors predicting the therapeutic efficacy of gefitinib in patients with epidermal growth factor receptor mutation-positive non-small cell lung cancer.

Background: Many randomized clinical trials have demonstrated that epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are advantageous over standard chemotherapy, either as front-line treatment or as further management of patients with EGFR mutation-positive non-small-cell lung cancer (NSCLC). However, which subgroup of these patients could benefit more from EGFR-TKIs needs to be further explored. In the present study, we explored the predictive factors in such cohorts of patients who received gefitinib.

Mitochondrial genome evolution and tRNA truncation in Acariformes mites: new evidence from eriophyoid mites.

The subclass Acari (mites and ticks) comprises two super-orders: Acariformes and Parasitiformes. Most species of the Parasitiformes known retained the ancestral pattern of mitochondrial (mt) gene arrangement of arthropods, and their mt tRNAs have the typical cloverleaf structure. All of the species of the Acariformes known, however, have rearranged mt genomes and truncated mt tRNAs.

DNA barcoding reveals the protogyne and deutogyne of Tegolophus celtis sp. nov. (Acari: Eriophyidae).

A few eriophyoid mites have two forms of adult female, called protogyne and deutogyne. The latter form is thought to increase survival under unfavorable conditions. The two forms have distinct morphological characters, which often cause them to be recognized as different species.

Classification and regression tree analysis of patients with non-small-cell lung cancer treated with gefitinib after chemotherapy.

Background: Many randomized studies have shown that epidermal growth factor receptor (EGFR-) tyrosine kinase inhibitors (TKIs) are apparently advantageous over standard chemotherapy in non-small-cell lung cancer (NSCLC) patients with EGFR active mutation in front-line treatment. But which subgroup of advanced NSCLC could benefit from EGFR-TKIs in the second-or third-line setting remains elusive. To explore predictive factors of advanced NSCLC patients with the unknown status of EGFR mutation treated by gefitinib in the second-or third-line setting, we used classification and regression tree (CART) analysis to screen which patients would benefit more.

Eriophyoid mites from Northeast China (Acari: Eriophyoidea).

We describe and illustrate herein one new genus and eighteen new eriophyoid mite species (Acari: Eriophyoidea) collected in northeast China. They are: Shevtchenkella huzhongiensis sp. nov.

Rhabdastrellic acid-A induced autophagy-associated cell death through blocking Akt pathway in human cancer cells.

Background: Autophagy is an evolutionarily conserved protein degradation pathway. A defect in autophagy may contribute to tumorigenesis. Autophagy inducers could have a potential function in tumor prevention and treatment.

A phase II trial of docetaxel plus nedaplatin and 5-fluorouracil in treating advanced esophageal carcinoma.

Background And Objective: Accumulating data indicate that docetaxel plus cisplatin and 5-fluorouracil has certain effect on advanced gastric or gastro-oesophageal junction adenocarcinoma. This study was to evaluate the efficacy and toxicity of docetaxel plus nedaplatin and 5-fluorouracil (DNF regimen) in treating advanced esophageal carcinoma.

Methods: Forty-three patients with pathologically confirmed advanced esophageal carcinoma treated by DNF regimen: intravenous infusion of docetaxel (75 mg/m(2)) over 1 h, intravenous infusion of nedaplatin (100 mg/m(2)) over 3 h, intravenous infusion of leucovorin (CF, 200 mg/m(2)) over 2 h, intravenous injection of 5-fluorouracil (375 mg/m(2)) over 10 min, followed by a 46-hour infusion of 5-fluorouracil (2.

Rhabdastrellic acid-A inhibited PI3K/Akt pathway and induced apoptosis in human leukemia HL-60 cells.

Increasing evidence suggests that aberrant activation of PI3K/Akt is involved in many human cancers, and that inhibition of the PI3K/Akt pathway might be a promising strategy for cancer treatment. Our investigation indicates that Rhabdastrellic acid-A, an isomalabaricane triterpenoid isolated from the sponge, Rhabdastrella globostellata, inhibits proliferation of HL-60 cells with an IC(50) value of 0.68mug/ml, and induces apoptosis.

[Apoptosis of human leukemia HL-60 cells induced by rhabdastrellic acid-A and its mechanisms].

Background & Objective: Rhabdastrellic acid-A is an isomalabaricane triterpenoid isolated from the sponge Rhabdastrella globostellata from South China Sea. Our previous study indicated that rhabdastrellic acid-A can inhibit the proliferation of many types of tumor cells with minor toxicity. This study was to investigate the apoptosis of human leukemia HL-60 cells induced by rhabdastrellic acid-A and its possible mechanisms.