Development of a triple NanoPCR method for feline calicivirus, feline panleukopenia syndrome virus, and feline herpesvirus type I virus.

Background: Feline calicivirus (FCV), Feline panleukopenia virus (FPV), and Feline herpesvirus type I (FHV-1) are the three most common pathogens in cats, and also are the main pathogens leading to the death of kittens. Here, by a combination of gold nanoparticles and conventional PCR, we established a novel triple NanoPCR molecular detection method for clinical detection.

Results: The triple NanoPCR molecular detection is able to detect 2.

Radially polarized cosine non-uniformly correlated beams and their propagation properties.

We introduce a kind of radially polarized partially coherent (RPPC) beam with a prescribed non-uniform correlation function, called a radially polarized cosine non-uniformly correlated (RPCNUC) beam. Based on the extended Huygens-Fresnel principle, we study the propagation properties in free space and in a turbulent atmosphere. Unlike RPPC beams with uniform coherence, RPCNUC beams possess the invariance of dark hollow cores and radial polarization, and exhibit self-focusing properties.

Multi-hyperbolic sine-correlated beams and their statistical properties in turbulent atmosphere.

We introduce a partially coherent beam, called a multi-hyperbolic sine-correlated (MHSC) beam, by employing a multi-hyperbolic sine function to modulate the spectral degree of coherence. Based on the extended Huygens-Fresnel principle and second-order moments of the Wigner distribution function, we derive the analytical expressions for the spectral intensity, the root-mean-square (rms) angular width and the factor in turbulent atmosphere. Numerical results show that the intensity profile, which keeps the dark-hollow invariant in free space, will be gradually destroyed by the turbulence along the propagation distance.

Propagation properties of radially polarized multi-Gaussian Schell-model beams in oceanic turbulence.

By utilizing the extended Huygens-Fresnel principle, we derive the analytical formulas for the cross-spectral density matrix elements of a radially polarized multi-Gaussian Schell-model (RPMGSM) beam propagating in oceanic turbulence. Effects of beam parameters and oceanic turbulence parameters on the propagation properties of RPMGSM beams are investigated in detail by numerical simulation. Our results show that the RPMGSM beam with larger beam order has an advantage over the radially polarized Gaussian Schell-model beam for reducing turbulence-induced degradation.

Construction method through multiple off-axis parabolic surfaces expansion and mixing to design an easy-aligned freeform spectrometer.

The classic Czerny-Turner spectrometer consists of a plane grating and two spherical mirrors. The optical path geometry adopted for incident and grating dispersed light is off-axis reflection, so the spherical collimating and focusing mirrors introduce coma and astigmatism. The conventional configuration is asymmetrical for coma automatic compensation, but suffers from astigmatism.

Customized design and efficient fabrication of two freeform aluminum mirrors by single point diamond turning technique.

The precision single point diamond turning technique has been a promising technology for generating small and medium-sized freeform optical elements with high surface quality. In this paper, we present an extremely off-axis freeform optical system with a large 10.0 mm pupil diameter and a low 3.

Modal wavefront estimation from its slopes by numerical orthogonal transformation method over general shaped aperture.

Wavefront estimation from the slope-based sensing metrologies zis important in modern optical testing. A numerical orthogonal transformation method is proposed for deriving the numerical orthogonal gradient polynomials as numerical orthogonal basis functions for directly fitting the measured slope data and then converting to the wavefront in a straightforward way in the modal approach. The presented method can be employed in the wavefront estimation from its slopes over the general shaped aperture.

Comparative assessment of orthogonal polynomials for wavefront reconstruction over the square aperture.

Four orthogonal polynomials for reconstructing a wavefront over a square aperture based on the modal method are currently available, namely, the 2D Chebyshev polynomials, 2D Legendre polynomials, Zernike square polynomials and Numerical polynomials. They are all orthogonal over the full unit square domain. 2D Chebyshev polynomials are defined by the product of Chebyshev polynomials in x and y variables, as are 2D Legendre polynomials.