Rapid and accurate detection of carbosulfan residues in vegetables is important for ensuring food safety. Herein, based on the unique hydrolysis behavior of carbosulfan, a nanozyme-based colorimetric sensing platform was proposed for detection of carbosulfan. Hollow polydopamine/MnO nanoparticles (H-PDA/MnO NPs) with excellent oxidase-like activity were synthesized, which can promote the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxidative product (oxTMB).
View Article and Find Full Text PDFBackground: Microcystin-leucine-arginine (MC-LR) produced by various cyanobacteria during harmful algal bloom poses serious threats to drinking water safety and human health. Conventional chromatography-based detection methods require expensive instruments and complicated sample pretreatment, limiting their application for on-site detection. Colorimetric aptasensors are simple and rapid, and are amenable to fast detection.
View Article and Find Full Text PDFThe acquisition of high quality lyophilized IgY products, characterized by an aesthetically pleasing visage, heightened stability, and a marked preservation of activity, constitutes an indispensable pursuit in augmenting the safety and pragmatic utility of IgY. Within this context, an exploration was undertaken to investigate an innovative modality encompassing microwave freeze-drying (MFD) as a preparatory methodology of IgY. Morphological assessments revealed that both cryogenic freezing and subsequent MFD procedures resulted in aggregation of IgY, with the deleterious influence posed by the MFD phase transcending that of the freezing phase.
View Article and Find Full Text PDFThe peroxidase-like activity of TiC nanosheets (TiC NSs) was evaluated by catalytic oxidation of colorless o-phenylenediamine (OPD) into orange-yellow 2,3-diaminophenazine (DAP) with the aid of HO. The catalytic behavior followed the typical Michaelis-Menten kinetics. Systematic studies about the catalytic activity of TiC NSs including cytochrome C (Cyt C) electron transfer experiments, radical capture experiments, and fluorescence analysis were conducted, revealing that the catalytic mechanism of TiC NSs was attributed to nanozyme-accelerated electron transfer between substrates and nanozyme-promoted generation of active species (superoxide anion free radical (·O) and holes (h)).
View Article and Find Full Text PDFCoriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C.
View Article and Find Full Text PDFWood decay resistance (WDR) is marking the value of wood utilization. Many trees of the Lauraceae have exceptional WDR, as evidenced by their use in ancient royal palace buildings in China. However, the genetics of WDR remain elusive.
View Article and Find Full Text PDFMost species have clearly defined distribution ranges and ecological niches. The genetic and ecological causes of species differentiation and the mechanisms that maintain species boundaries between newly evolved taxa and their progenitors are, however, less clearly defined. This study investigated the genetic structure and clines in Pinus densata, a pine of hybrid origin on the southeastern Tibetan Plateau, to gain an understanding of the contemporary dynamics of species barriers.
View Article and Find Full Text PDFQuercus dentata Thunb., a dominant forest tree species in northern China, has significant ecological and ornamental value due to its adaptability and beautiful autumn coloration, with color changes from green to yellow into red resulting from the autumnal shifts in leaf pigmentation. However, the key genes and molecular regulatory mechanisms for leaf color transition remain to be investigated.
View Article and Find Full Text PDFThe genus (Ericaceae), with more than 1000 species highly diverse in flower color, is providing distinct ornamental values and a model system for flower color studies. Here, we investigated the divergence between two parental species with different flower color widely used for azalea breeding. Gapless genome assembly was generated for the yellow-flowered azalea, .
View Article and Find Full Text PDFA recyclable peroxidase mimic FeO@polydopamine/Prussian blue (FeO@PDA/PB) composite was facilely prepared by coating PDA on an FeO nanoparticle core and growth of PB nanoparticles on a PDA shell. The prepared FeO@PDA/PB composite exhibited excellent peroxidase-like activity and can catalytically oxidize the colorless colorimetric substrate 3,3',5,5'-tetramethylbenzidine (TMB) into a blue colored product in the presence of HO at 30 °C in 1 min. The catalytic mechanism was deduced to be the nanozyme-promoted generation of a hydroxyl radical (·OH), and the catalytic behavior followed the typical Michaelis-Menten kinetics.
View Article and Find Full Text PDFThe preparation of egg yolk powder (EYP) with excellent solubility and high retention of active IgY is of great significance for increasing the added value and promoting the application of EYP. A new method of preparing EYP by microwave-assisted freeze-drying (MFD) was researched. Confocal laser scanning microscopy results demonstrated that the supplementation of excipients (sucrose, trehalose, and maltodextrin) could inhibit lipoproteins aggregation in egg yolk induced by freezing.
View Article and Find Full Text PDFAg nanoparticle-decorated TiC nanosheets (AgNPs@TiC NSs) were facilely synthesized via a self-reduction approach, in which TiC NSs acted as both reductant and supporter. The AgNPs@TiC NS nanocomposite exhibited excellent peroxidase-like activity with o-phenylenediamine (OPD) and HO as substrates. The catalytic behavior followed the typical Michaelis-Menten kinetics; Michaelis constant (K) and maximum initial velocity (V) for OPD were 0.
View Article and Find Full Text PDFThe existence of cyanotoxins poses serious threats to human health, it is highly desirable to develop specific and sensitive methods for rapid detection of cyanotoxins in food and water. Due to the distinct advantages of aptamer including high specificity, good stability and easy preparation, various aptamer-based sensors (aptasensors) have been proposed to promote the detection of cyanotoxins. In this review, we summarize recent advance in optical and electrochemical aptasensors for cyanotoxins sensing by integrating with versatile nanomaterials or innovative sensing strategies, such as colorimetric aptasensors, fluorescent aptasensors, surface enhancement Raman spectroscopy-based aptasensors, voltammetric aptasensors, electrochemical impedance spectroscopy-based aptasensors and photoelectrochemical aptasensors.
View Article and Find Full Text PDFPolyploidization plays a key role in plant evolution, but the forces driving the fate of homoeologs in polyploid genomes, i.e., paralogs resulting from a whole-genome duplication (WGD) event, remain to be elucidated.
View Article and Find Full Text PDFGinger (Zingiber officinale) is one of the most valued spice plants worldwide; it is prized for its culinary and folk medicinal applications and is therefore of high economic and cultural importance. Here, we present a haplotype-resolved, chromosome-scale assembly for diploid ginger anchored to 11 pseudochromosome pairs with a total length of 3.1 Gb.
View Article and Find Full Text PDFAzaleas (Ericaceae) comprise one of the most diverse ornamental plants, renowned for their cultural and economic importance. We present a chromosome-scale genome assembly for Rhododendron simsii, the primary ancestor of azalea cultivars. Genome analyses unveil the remnants of an ancient whole-genome duplication preceding the radiation of most Ericaceae, likely contributing to the genomic architecture of flowering time.
View Article and Find Full Text PDFBackground: Traumatic brain injury (TBI) triggers both immediate (primary) and long-term (secondary) tissue damages. Secondary damages can last from hours to days or even a lifetime. Secondary damages implicate several mechanisms, including influence of inflammatory mediators, mainly cytokines, on excitability of ion channels.
View Article and Find Full Text PDFAim: We aimed to investigate the importance of early diagnosis and proper management of paradoxical herniation based on the data of 13 patients who had 14 occurrences of paradoxical herniation.
Material And Methods: The characteristics and the effectiveness of treatments of 13 patients with paradoxical herniation were reviewed and analyzed retrospectively.
Results: Paradoxical herniation occurred in eight patients (61.
Paradoxical herniation (PH) is a life-threatening emergency after decompressive craniectomy. In the current study, we examined patient survival in patients who developed PH after decompressive craniectomy versus those who did not. Risk factors for, and management of, PH were also analyzed.
View Article and Find Full Text PDFInterleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour exposure of interleukin-6 on cortical neurons at various concentrations (0.1, 1, 5 and 10 ng/mL) and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations (2, 4, 8, 24 and 48 hours) by studying voltage-gated Na(+) channels using a patch-clamp technique.
View Article and Find Full Text PDFBackground: Previous studies showed that TNF-α could activate voltage-gated Na(+) channels (VGSCs) in the peripheral nervous system (PNS). Since TNF-α is implicated in many central nervous system (CNS) diseases, we examined potential effects of TNF-α on VGSCs in the CNS.
Methods: Effects of TNF-α (1-1000 pg/mL, for 4-48 h) on VGSC currents were examined using whole-cell voltage clamp and current clamp techniques in primary culture of mouse cortical neurons.
Biochem Biophys Res Commun
July 2014
Typical antipsychotics are characterized by extrapyramidal syndrome (EPS). Previous studies demonstrated that typical antipsychotics could inhibit neuronal voltage-gated sodium channel (VGSC). However, EPS typically emerge only upon prolonged exposure.
View Article and Find Full Text PDFHua Xi Kou Qiang Yi Xue Za Zhi
October 2013
Objective: This study aims to analyze the biomechanical effects of four sutures that are related to maxillary growth during a maxillary protraction treatment cycle based on labiolingual appliance.
Methods: A three-dimensional simulation of labiolingual appliance was performed based on a three-dimensional finite-element maxillofacial model combined with solid-modeled sutures.
Results: The displacement and stress distributions were calculated.
Zhongguo Dang Dai Er Ke Za Zhi
April 2009
Objective: To study serum levels of melatonin in children with epilepsy or febrile seizures in order to provide a basis for the treatment of epilepsy or febrile seizures with melatonin.
Methods: Serum melatonin levels were measured using ELISA in 15 children with simple febrile seizure (SFS), in 15 children with complex febrile seizure (CFS), in 15 children with epilepsy, and in 15 children with upper respiratory infections (control group).
Results: Serum melatonin levels in children with epilepsy (8.
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