Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging.

Mitochondrial Rho-GTPase 1 (MIRO1) is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis. In present study, we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging. We found that MIRO1 expressed in mouse and porcine oocytes, and its expression decreased in aged mice.

Kinesin KIF3A regulates meiotic progression and spindle assembly in oocyte meiosis.

Kinesin family member 3A (KIF3A) is a microtubule-oriented motor protein that belongs to the kinesin-2 family for regulating intracellular transport and microtubule movement. In this study, we characterized the critical roles of KIF3A during mouse oocyte meiosis. We found that KIF3A associated with microtubules during meiosis and depletion of KIF3A resulted in oocyte maturation defects.

Music therapy and anxiety: A bibliometric review from 1993 to 2023.

Background: Music therapy (MT) has received increasing attention from scholars in the efficacy treatment of anxiety symptoms, which is of great significance to human physical and mental health. The visual mapping functionality of CiteSpace and Vosviewer software was applied in this study to assess the status of MT in the treatment of anxiety symptoms.

Methods: In order to find research on MT and anxiety that were relevant for this research, we searched the Web of Science database.

Aluminum exposure impairs oocyte quality via subcellular structure disruption and DNA damage-related apoptosis in mice.

Aluminum (Al) can lead to an exposure of creature in varieties ways for its universality, and it could disturb normal physiological metabolism, with the damage to multisystem including reproduction. Since the oocyte quality is critical for female reproduction, we inspected the toxicity of Al on mouse oocyte maturation. We constructed in vitro exposure mouse model, and we found that 5 mmol/L Al had adverse effects on oocyte maturation by impairing organelle and cytoskeleton.

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.

ADP-ribosylation factor 1 (Arf1) is a small GTPase belonging to the Arf family. As a molecular switch, Arf1 is found to regulate retrograde and intra-Golgi transport, plasma membrane signaling, and organelle function during mitosis. This study aimed to explore the noncanonical roles of Arf1 in cell cycle regulation and cytoskeleton dynamics in meiosis with a mouse oocyte model.

Theory, framework, and methodology for physical lifespan prediction of hazardous waste landfills.

Landfill is a predominant method for hazardous waste disposal in both developed and emerging economies due to simple disposal technology and wide applicability. The prediction of landfill lifespan during the design stage provides support for environmental management of hazardous waste landfill (HWL) and technical support for the implementation of national standards. It also provides guidance for necessary responses after the lifespan expires.

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis.

Microspherule protein 1 (Mcrs1) is a component of the nonspecific lethal (NSL) complex and the chromatin remodeling INO80 complex, which participates in transcriptional regulation during mitosis. Here, we investigate the roles of Mcrs1 during female meiosis in mice. We demonstrate that Mcrs1 is a novel regulator of the meiotic G2/M transition and spindle assembly in mouse oocytes.

Kinesin KIF15 regulates tubulin acetylation and spindle assembly checkpoint in mouse oocyte meiosis.

Microtubule dynamics ensure multiple cellular events during oocyte meiosis, which is critical for the fertilization and early embryo development. KIF15 (also termed Hklp2) is a member of kinesin-12 family motor proteins, which participates in Eg5-related bipolar spindle formation in mitosis. In present study, we explored the roles of KIF15 in mouse oocyte meiosis.

Long-term degradation characteristics of cyanide in closed monofills and its effects on the environment and human health: Evidence from nine landfill sites in northen China.

Cyanide residues weighing many millions of tons are disposed of in cyanide residue monofills (CRMs) worldwide. The degradation characteristics of cyanide in the anoxic environments of closed landfills may have been overestimated, leading to an underestimation of the long-term risk of cyanide residue landfills. To study the effect, a total of 387 cyanide residue samples were collected for analysis from nine closed CRMs in northen China that have been closed for more than 10 years.

High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.

Zearalenone is a mycotoxin produced by fungi of the genus Fusarium, which has severe toxicity on animal and human health including reproduction. Previous study showed that zearalenone exposure inhibited oocyte polar body extrusion, while in present study we found that high dose zearalenone disturbed oocyte meiosis resumption. Our results showed that a high concentration of 100 μM zearalenone reduced the rate of germinal vesicle (GV) breakdown in mouse oocytes.

Facile Synthesis of Porous g-CN with Enhanced Visible-Light Photoactivity.

Porous graphitic carbon nitride (g-CN) was prepared by dicyandiamide and urea via the pyrolysis method, which possessed enhanced visible-light-driven photocatalytic performance. Its surface area was increased from 17.12 to 48.

LRRK2 regulates actin assembly for spindle migration and mitochondrial function in mouse oocyte meiosis.

Leucine-rich-repeat kinase 2 (LRRK2) belongs to the Roco GTPase family and is a large multidomain protein harboring both GTPase and kinase activities. LRRK2 plays indispensable roles in many processes, such as autophagy and vesicle trafficking in mitosis. In this study, we showed the critical roles of LRRK2 in mammalian oocyte meiosis.

Melatonin reverses mitochondria dysfunction and oxidative stress-induced apoptosis of Sudan I-exposed mouse oocytes.

Sudan I is one of the industry dyes and widely used in cosmetics, wax agent, solvent and textile. Sudan I has multiple toxicity such as carcinogenicity, mutagenicity, genotoxicity and oxidative damage. However, Sudan I has been illegally used as colorant in food products, triggering worldwide attention about food safety.

DEHP exposure to lactating mice affects ovarian hormone production and antral follicle development of offspring.

Di (2-ethylhexyl) phthalate (DEHP) is widely used as a plastic additive and it could induce reproduction defects and fertility in mammals as environmental endocrine disruptor. However, the effects and potential mechanism of DEHP exposure during lactation stage on follicular development of offspring are still unclear. In this study, we found that the total primordial follicle number and antral follicles in the suckling of mice exposed to DEHP during lactation was significantly reduced.

Fumonisin B1 exposure deteriorates oocyte quality by inducing organelle dysfunction and DNA damage in mice.

Oocyte quality is critical for fertilization and early embryo development. Fumonisin B1 (FB1) is a Fusarium mycotoxin and it is commonly found in contaminated food and feedstuff, posing a potential health hazard to both animals and human. FB1 is reported to have hepatotoxicity, neurotoxicity, nephrotoxicity, immunotoxicity and embryotoxicity.

A general framework and practical procedure for improving pxrf measurement accuracy with integrating moisture content and organic matter content parameters.

Rapid, accurate detection of heavy-metal content is extremely important for precise risk control and targeted remediation. Herein, a general modeling method and process based on the relationship between Pxrf measured values and site parameters are explored to construct a Pxrf correction model suitable to improve each site's measurement accuracy. Results show a significant correlation between Pb, Mn, and Zn Pxrf measured values and actual concentrations, with correlation coefficients between 0.

Increased Environment-Related Metabolism and Genetic Expression in the Matured Mouse Oocytes by Transcriptome Analysis.

Infertility in humans at their reproductive age is a world-wide problem. Oocyte maturation (IVM) is generally used in such cases to acquire the embryo in assisted reproductive technology (ART). However, the differences between an (IVO) and IVM culture environment in the RNA expression profile in oocytes, remains unclear.

Antioxidant depletion patterns of high-density polyethylene geomembranes in landfills under different exposure conditions.

The service life of high-density polyethylene (HDPE) geomembranes is directly determined by the landfill environment, and the antioxidant depletion stage is the first and most important stage of the HDPE geomembrane aging process. In this study, the antioxidant depletion stage was chosen to investigate the effects of different exposure environments on the HDPE geomembrane lifespan. The antioxidant depletion rate (ADR) and the antioxidant depletion time (ADT) of HDPE geomembranes under various exposure conditions were calculated based on the aging parameters obtained by fitting the collected reported data with the Arrhenius model.

Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice.

Primordial follicle assembly in the mouse occurs during perinatal ages and largely determines the ovarian reserve that will be available to support the reproductive life span. The development of primordial follicles is controlled by a complex network of interactions between oocytes and ovarian somatic cells that remain poorly understood. In the present research, using single-cell RNA sequencing performed over a time series on murine ovaries, coupled with several bioinformatics analyses, the complete dynamic genetic programs of germ and granulosa cells from E16.

Di (2-ethylhexyl) phthalate impairs primordial follicle assembly by increasing PDE3A expression in oocytes.

It is known that Di (2-ethylhexyl) phthalate (DEHP) may impact mammalian reproduction and that in females one target of the drug's action is follicle assembly. Here we revisited the phthalate's action on the ovary and from bioinformatics analyses of the transcriptome performed on newborn mouse ovaries exposed in vitro to DEHP, up-regulation of PDE3A, as one of the most important alterations caused by DEHP on early folliculogenesis, was identified. We obtained some evidence suggesting that the decrease of cAMP level in oocytes and the parallel decrease of PKA expression, consequent on the PDE3A increase, were a major cause of the reduction of follicle assembly in the DEHP-exposed ovaries.

Framework, method and case study for the calculation of end of life for HWL and parameter sensitivity analysis.

Mass construction and operation of hazardous waste landfill infrastructure has greatly improved China's waste management and environmental safety. However, the deterioration of engineering materials and the failure of landfill may lead to the release of untreated leachate rich in persistent toxic pollutants to the soil and shallow groundwater. Accordingly, we develop the framework and process model to predict landfill life by coupling the landfill hydrological performance model and material degradation model.

Di (2-ethylhexyl) Phthalate Exposure Impairs the microRNAs Expression Profile During Primordial Follicle Assembly.

This research was performed to estimate the potential effects of Di (2-ethylhexyl) phthalate (DEHP) on changes of ovarian miRNA expression profile during mouse primordial follicle assembly using miRNAs-seq analysis. The ovaries of newborn mice were collected and cultured with different concentration of DEHP for 72 h. Then they were prepared for miRNAs-seq analysis.

Isolation distance between municipal solid waste landfills and drinking water wells for bacteria attenuation and safe drinking.

Groundwater pollution and human health risks caused by leachate leakage have become a worldwide environmental problem, and the harm and influence of bacteria in leachate have received increased attention. Setting the isolation distance between landfill sites and groundwater isolation targets is particularly important. Firstly, the intensity model of pollutant leakage source and solute transport model were established for the isolation of pathogenic Escherichia coli.

Complex resistivity characteristics of saltwater-intruded sand contaminated by heavy metal.

Different pollutants affect electrical characteristics of soil, e.g., electric resistivity and capacity.

Metagenomic analysis of gut microbiota alteration in a mouse model exposed to mycotoxin deoxynivalenol.

As one of the most prevalent contaminants in animal and human food, the deleterious effects of trichothecene mycotoxin deoxynivalenol (DON) warrant extensive investigation. Here, to assess the effects of DON exposure to the populations of gut microbiota, four-weeks-old mice were exposed to different doses (1.0 and 5.