Molecular characterization of NDM-1-producing carbapenem-resistant complex from a tertiary hospital in Chongqing, China.

Background: The aim of this study was to clarify the molecular characterization of NDM-1-producing carbapenem-resistant complex (CREL) at a teaching hospital in Chongqing, China.

Methods: Antimicrobial susceptibility and resistance genes were analyzed. Epidemiological relationship was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).

An Outbreak of Carbapenem-Resistant in an Intensive Care Unit of a Major Teaching Hospital in Chongqing, China.

Background: Due to the critical condition and poor immunity of patients, the intensive care unit (ICU) has always been the main hospital source of multidrug-resistant bacteria. In recent years, with the large-scale use of antibiotics, the detection rate and mortality of carbapenem-resistant (CRKP) have gradually increased. This study explores the molecular characteristics and prevalence of CRKP isolated from the ICU ward of a tertiary hospital in China.

Heat inactivation decreases the qualitative real-time RT-PCR detection rates of clinical samples with high cycle threshold values in COVID-19.

SARS-CoV-2 has caused COVID-19 pandemic globally in the beginning of 2020, and qualitative real-time RT-PCR has become the gold standard in diagnosis. As SARSCoV-2 with strong transmissibility and pathogenicity, it has become a professional consensus that clinical samples from suspected patients should be heat inactivated at 56°C for 30 min before further processing. However, previous studies on the effect of inactivation on qualitative real-time RT-PCR were conducted with diluted samples rather than clinical samples.

Clinical features of discharged COVID-19 patients with an extended SARS-CoV-2 RNA positive signal in respiratory samples.

• Patients may remain positive for SARS-CoV-2 RNA in respiratory samples for some time after hospital discharge; • Although this signal may represent non-infectious virus, it seems safe to quarantine recovered patients for at least two weeks; • Stricter criteria for hospital discharge may be warranted.