Mediating effect of social support in the relationship of symptom burden and fear of disease progression in stroke patients.

Aims: To investigate the current status and influencing factors of fear of disease progression in stroke patients, and to analyze the mediating effect of social support between symptom burden and the fear of disease progression.

Methods: A cross-sectional study recruited 235 stroke patients in a convenience sample from the neurology department of a tertiary hospital in China. The general information questionnaire, social support rating scale, stroke symptom experience scale, and fear of progression questionnaire short form were utilized for the survey.

A pivotal switch in β-domain determines the substrate selectivity of terpene synthases involved in Gardenia jasminoides floral scent synthesis.

Plants have evolved a diverse array of secondary metabolites to enhance their adaptability to environmental stresses, with volatile terpenoids being a notable example. Gardenia (Gardenia jasminoides), celebrated for its unique fragrance, is a key natural source of volatile terpenoids. Using our chromosome-level genome and transcriptome data of G.

Convergent evolution of berberine biosynthesis.

Berberine is an effective antimicrobial and antidiabetic alkaloid, primarily extracted from divergent botanical lineages, specifically (Ranunculales, early-diverging eudicot) and (Sapindales, core eudicot). In comparison with its known pathway in species, its biosynthesis in species remains elusive. Using chromosome-level genome assembly, coexpression matrix, and biochemical assays, we identified six key steps in berberine biosynthesis from , including methylation, hydroxylation, and berberine bridge formation.

Novel Differentially Expressed LncRNAs Regulate Artemisinin Biosynthesis in .

Long non-coding RNAs (lncRNAs) are crucial in regulating secondary metabolite production in plants, but their role in artemisinin (ART) biosynthesis, a key anti-malarial compound from Artemisia annua, remains unclear. Here, by investigating high-artemisinin-producing (HAP) and lowartemisinin-producing (LAP) genotypes, we found that the final artemisinin content in is influenced by the quantity of the precursor compounds. We report on RNA deep sequencing in HAP and LAP genotypes.

Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.

Background: Licorice is extensively and globally utilized as a medicinal herb and is one of the traditional Chinese herbal medicines with valuable pharmacological effects. Its therapeutic components primarily reside within its roots and rhizomes, classifying it as a tonifying herb. As more active ingredients in licorice are unearthed and characterized, licorice germplasm resources are gaining more and more recognition.

Multi-Omics Revealed Regulatory Mechanisms Underlying the Flowering of across Three Dimensions.

Ferula spp. is an essential crop in Central Asia with pronounced economic benefits governed by its flowering process. However, the mechanisms of the flowering phenotype remain unclear.

Analysis of Whole-Genome for Identification of Seven Species with Significant Economic Value.

The genus exhibits a broad global distribution and holds substantial economic value in sectors including agriculture, industry, and medicine. Particularly in agriculture, species significantly impact plants, causing diseases and contamination that adversely affect crop yields and quality. Timely detection of species is crucial for controlling disease and preventing mycotoxins from entering the food chain.

Low-Light Sparse Polarization Demosaicing Network (LLSPD-Net): Polarization Image Demosaicing Based on Stokes Vector Completion in Low-Light Environment.

Polarization imaging has achieved a wide range of applications in military and civilian fields such as camouflage detection and autonomous driving. However, when the imaging environment involves a low-light condition, the number of photons is low and the photon transmittance of the conventional Division-of-Focal-Plane (DoFP) structure is small. Therefore, the traditional demosaicing methods are often used to deal with the serious noise and distortion generated by polarization demosaicing in low-light environment.

Trajectory patterns and influencing factors of supportive care needs in stroke patients: A longitudinal study.

Aim: To investigate the trajectory patterns and influencing factors of supportive care needs in stroke patients.

Design: A longitudinal study.

Methods: In total, 207 stroke patients who received treatment at the Department of Neurology in a hospital in Xuzhou between July 2022 and July 2023 were recruited using convenience sampling.

genome reveals the evolutionary origin of crocin biosynthesis.

(saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. specialized metabolites, crocins, are biosynthesized in distant species, (eudicot) and (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids.

Comparative genomics and phylogenomics of the genus (Fabaceae) based on chloroplast genomes.

(Fabaceae) species are rich in metabolites and widely used in medicine. Research on the chloroplast genome of is important for understanding its phylogenetics, biogeography, genetic diversity, species identification, and medicinal properties. In this study, comparative genomics and phylogenomics of were analyzed based on the chloroplast genome.

Analysis of Whole-Genome facilitates rapid and precise identification of fungal species.

Fungal identification is a cornerstone of fungal research, yet traditional molecular methods struggle with rapid and accurate identification, especially for closely related species. To tackle this challenge, we introduce a universal identification method called Analysis of whole GEnome (AGE). AGE includes two key steps: bioinformatics analysis and experimental practice.

Lineage-Specific CYP80 Expansion and Benzylisoquinoline Alkaloid Diversity in Early-Diverging Eudicots.

Menispermaceae species, as early-diverging eudicots, can synthesize valuable benzylisoquinoline alkaloids (BIAs) like bisbenzylisoquinoline alkaloids (bisBIAs) and sinomenines with a wide range of structural diversity. However, the evolutionary mechanisms responsible for their chemo-diversity are not well understood. Here, a chromosome-level genome assembly of Menispermum dauricum is presented and demonstrated the occurrence of two whole genome duplication (WGD) events that are shared by Ranunculales and specific to Menispermum, providing a model for understanding chromosomal evolution in early-diverging eudicots.

Analysis of Whole-Genome as a Novel Strategy for Animal Species Identification.

Survival crises stalk many animals, especially endangered and rare animals. Accurate species identification plays a pivotal role in animal resource conservation. In this study, we developed an animal species identification method called Analysis of whole-GEnome (AGE), which identifies species by finding species-specific sequences through bioinformatics analysis of the whole genome and subsequently recognizing these sequences using experimental technologies.

Identification of closely related species in through Analysis of Whole-Genome.

The challenge of discriminating closely related species persists, notably within clinical diagnostic laboratories for invasive aspergillosis (IA)-related species and food contamination microorganisms with toxin-producing potential. We employed Analysis of the whole-GEnome (AGE) to address the challenges of closely related species within the genus and developed a rapid detection method. First, reliable whole genome data for 77 species were downloaded from the database, and through bioinformatic analysis, specific targets for each species were identified.

The Functional Characteristics and Soluble Expression of Saffron CCD2.

Crocins are important natural products predominantly obtained from the stigma of saffron, and that can be utilized as a medicinal compound, spice, and colorant with significant promise in the pharmaceutical, food, and cosmetic industries. Carotenoid cleavage dioxygenase 2 (CCD2) is a crucial limiting enzyme that has been reported to be responsible for the cleavage of zeaxanthin in the crocin biosynthetic pathway. However, the catalytic activity of CCD2 on β-carotene/lycopene remains elusive, and the soluble expression of CCD2 remains a big challenge.

Chemoselective and Divergent Synthesis of Chlorohydrins and Oxaheterocycles via Ir-Catalyzed Asymmetric Hydrogenation.

Chlorohydrins and oxaheterocycles are synthetically valuable building blocks for diverse natural products and therapeutic substances. A highly efficient Ir/-phamidol-catalyzed asymmetric hydrogenation of ω-chloroketones was successfully developed, and various chlorohydrins and oxaheterocycles were obtained divergently with excellent yields and enantioselectivities (up to >99% yield and >99% ee). Synthetic utilities of this divergent transformation were demonstrated by gram-scale synthesis of key intermediates of several enantiomerically enriched drugs via this catalytic methodology.

Cobalt-Catalyzed Enantioselective Hydrogenation of Diaryl Ketones with Ferrocene-Based Secondary Phosphine Oxide Ligands.

A new class of cobalt catalytic system for asymmetric hydrogenation of ketones was herein reported, involving the development of novel ferrocene-based secondary phosphine oxide ligands. An unusual P-O bidentate coordination pattern with cobalt was confirmed by an X-ray diffraction study. The bichelating tetrahedral cobalt(II) complexes afforded high reactivities (up to 99% yield) and good to excellent enantioselectivities (up to 92% ee) in the AH of various -substituted diaryl ketones.

A comprehensive analysis of the Bencao (herbal) small RNA Atlas reveals novel RNA therapeutics for treating human diseases.

Cross-kingdom herbal miRNA was first reported in 2012. Using a modified herbal extraction protocol, we obtained 73,677,287 sequences by RNA-seq from 245 traditional Chinese Medicine (TCM), of which 20,758,257 were unique sequences. We constructed a Bencao (herbal) small RNA (sRNA) Atlas ( http://bencao.

DNA barcoding in herbal medicine: Retrospective and prospective.

DNA barcoding has been widely used for herb identification in recent decades, enabling safety and innovation in the field of herbal medicine. In this article, we summarize recent progress in DNA barcoding for herbal medicine to provide ideas for the further development and application of this technology. Most importantly, the standard DNA barcode has been extended in two ways.

Gene Losses and Homology of the Chloroplast Genomes of and Species.

Research on the chloroplast genome of parasitic plants is limited. In particular, the homology between the chloroplast genomes of parasitic and hyperparasitic plants has not been reported yet. In this study, three chloroplast genomes of (, , and ) and one chloroplast genome of () were sequenced and analyzed, among which is the host of .

Application of third-generation sequencing to herbal genomics.

There is a long history of traditional medicine use. However, little genetic information is available for the plants used in traditional medicine, which limits the exploitation of these natural resources. Third-generation sequencing (TGS) techniques have made it possible to gather invaluable genetic information and develop herbal genomics.