Effects of supplementary light treatment on saffron: integrated physiological, metabolomic, and transcriptome analyses.

Background: Saffron (Crocus sativus L.) is a perennial, bulbous flower whose stigma is one of the most valuable spices, herbal medicines, and dyes. Light is an essential environmental regulator of plant growth, development, and metabolism.

Omeprazole and its analogs exhibit insecticidal potencies as inhibitors of insect choline acetyltransferase.

Choline acetyltransferase (ChAT) is crucial for acetylcholine synthesis and regulates diverse functions in numerous biological processes. Omeprazole, an inhibitor on human ChAT, was evaluated here on insect ChAT as a potential inhibitor, as well as its insecticidal potency on Nilaparvata lugens, a major insect pest on rice. The evaluation also included omeprazole analogs and α-NETA, in order to explore a superior leading compound targeting on insect ChAT.

Supportive care needs of parents caring for children with leukemia: a latent profile analysis.

Background: Parents' supportive care needs directly affect their quality of life and the quality of care for their children diagnosed with leukemia.

Objectives: To identify supportive care needs of Chinese parents in the unobserved subgroups who care for children with leukemia and to examine the associations of the latent class membership with individual characteristics.

Methods: A multicenter cross-sectional survey study was conducted.

Selectively Quantify Toxic Pollutants in Water by Machine Learning Empowered Electrochemical Biosensors.

Electroactive biofilm (EAB) sensors have become pivotal in water quality detection and early ecological risk warnings due to their remarkable sensitivity. However, it is challenging to identify multiple toxicants in complex water bodies concurrently. This research developed an innovative biosensor detection strategy combined with machine learning.

Pesticides enhance maternal care in the wolf spider by inhibiting HSD3B, an enzyme for progesterone synthesis.

Natural enemies are essential for the integrated pest management (IPM) strategy in insect pest control. However, chemical pesticides remain the primary method for insect pest control, diminishing natural enemies and causing environmental contamination. The wolf spider (Pardosa pseudoannulata), a major enemy in agro-ecosystem, exhibits maternal care (MC) behaviors such as eggsac-carrying and pulli-carrying, which are crucial for population growth.

Akt-FoxO signaling drives co-adaptation to insecticide and host plant stresses in an herbivorous insect.

Introduction: Ongoing interactions between host and herbivorous insect trigger a co-evolutionary arms race. Genetic diversity within insects facilitates their adaptation to phytochemicals and their derivatives, including plant-derived insecticides. Cytochrome P450s play important roles in metabolizing phytochemicals and insecticides, due to their diversity and almost perfect evolution.

Identifying Typologies of Symptoms and Functional Impairments in Chinese Children With Leukemia and Their Population Characteristics: A Latent Profile Analysis.

Background: Symptom distress and functional impairments in children with leukemia directly affect their quality of life.

Objectives: To identify subgroups of symptom distress and functional impairments in Chinese children with leukemia and to examine the associations of the latent classes with individual characteristics.

Methods: This multicenter cross-sectional survey study recruited children with leukemia who received chemotherapy in 4 hospitals in Shanghai, Jiangsu, Zhejiang, and Guangdong Province.

Nicotine promotes M2 macrophage polarization through α5-nAChR/SOX2/CSF-1 axis in lung adenocarcinoma.

α5-nicotinic acetylcholine receptor (α5-nAChR) plays a vital part in lung adenocarcinoma (LUAD). However, it is not comprehensively understood that how the α5-nAChR affects LUAD. Through diverse bioinformatics analyses and immunohistochemistry, the expressions of α5-nAChR and SOX2 as well as their relations were dissected.

Knockdown of Fzr inhibited the growth of Nilaparvata lugens by blocking endocycle.

Background: The endocycle can generate cells referred to as 'polyploid'. Fizzy-related protein (Fzr) plays an important role in driving the mitosis-to-endocycle transition. The brown planthopper (BPH), Nilaparvata lugens (Stål), a serious insect pest, feeds exclusively on rice.

Corneal stromal structure replicating humanized hydrogel patch for sutureless repair of deep anterior-corneal defect.

A critical shortage of donor corneas exists worldwide. Hydrogel patches with a biological architecture and functions that simulate those of native corneas have garnered considerable attention. This study introduces a stromal structure replicating corneal patch (SRCP) composed of a decellularized cornea-templated nanotubular skeleton, recombinant human collagen, and methacrylated gelatin, exhibiting a similar ultrastructure and transmittance (above 80 %) to natural cornea.

VO-Based Spacecraft Smart Radiator with High Emissivity Tunability and Protective Layer.

In the extreme space environment, spacecraft endure dramatic temperature variations that can impair their functionality. A VO-based smart radiator device (SRD) offers an effective solution by adaptively adjusting its radiative properties. However, current research on VO-based thermochromic films mainly focuses on optimizing the emissivity tunability (Δ) of single-cycle sandwich structures.

Dual-Responsive Epigenetic Inhibitor Nanoprodrug Combined with Oncolytic Virus Synergistically Boost Cancer Immunotherapy by Igniting Gasdermin E-Mediated Pyroptosis.

Cancer immunotherapy has emerged as a promising approach for the treatment of various cancers. However, the immunosuppressive tumor microenvironment (TME) limits the efficacy of current immunotherapies. In this study, we designed a dual-responsive DNA methyltransferase inhibitor nanoprodrug ACNPs for combination therapy with oncolytic herpes simplex virus (oHSV).

Legionella maintains host cell ubiquitin homeostasis by effectors with unique catalytic mechanisms.

The intracellular bacterial pathogen Legionella pneumophila modulates host cell functions by secreting multiple effectors with diverse biochemical activities. In particular, effectors of the SidE family interfere with host protein ubiquitination in a process that involves production of phosphoribosyl ubiquitin (PR-Ub). Here, we show that effector LnaB converts PR-Ub into ADP-ribosylated ubiquitin, which is further processed to ADP-ribose and functional ubiquitin by the (ADP-ribosyl)hydrolase MavL, thus maintaining ubiquitin homeostasis in infected cells.

Legionella maintains host cell ubiquitin homeostasis by effectors with unique catalytic mechanisms.

The reversal of ubiquitination induced by members of the SidE effector family of produces phosphoribosyl ubiquitin (PR-Ub) that is potentially detrimental to host cells. Here we show that the effector LnaB functions to transfer the AMP moiety from ATP to the phosphoryl moiety of PR-Ub to convert it into ADP-ribosylated ubiquitin (ADPR-Ub), which is further processed to ADP-ribose and functional ubiquitin by the (ADP-ribosyl)hydrolase MavL, thus maintaining ubiquitin homeostasis in infected cells. Upon being activated by Actin, LnaB also undergoes self-AMPylation on tyrosine residues.

Deciphering the differentiated performance on electricity generation and COD degradation by Rhodopseudomonas-dominated bioanode in light or dark.

Anoxygenic phototrophic bacteria is a promising catalyst for constructing bioanode, but the mixed culture with non-photosynthetic bacteria is inevitable in an open environment application. In this study, a Rhodopseudomonas-dominated mixed culture with other electrogenic bacteria was investigated for deciphering the differentiated performance on electricity generation in light or dark conditions. The kinetic study showed that reaction rate of OM degradation was 9 times higher than that under dark condition, demonstrating that OM degradation was enhanced by photosynthesis.

Enhanced Microbial Protein Production from CO and Air by a MoS Catalyzed Bioelectrochemical System.

Carbon dioxide can be relatively easily reduced to organic matter in a bioelectrochemical system (BES). However, due to insufficient reduction force from in-situ hydrogen evolution, it is difficult for nitrogen reduction. In this study, MoS was firstly used as an electrocatalyst for the simultaneous reduction of CO and N to produce microbial protein (MP) in a BES.

Predictive biomarkers for immune-related adverse events in cancer patients treated with immune-checkpoint inhibitors.

Purpose: The objective of this study was to identify potential predictors of immune-related adverse events (irAEs) in cancer patients receiving immune checkpoint inhibitor therapy among serum indexes, case data, and liquid biopsy results.

Methods: We retrospectively analyzed 418 patients treated with anti-programmed cell death 1(PD-1)/PD-1 ligand (PD-L1) inhibitors from January 2018 to May 2022 in our cancer center. We identified factors that correlated with the occurrence of irAEs and evaluated associations between irAEs and anti-PD-1/PD-L1 inhibitor responses.

Chromosome-level genome of spider Pardosa pseudoannulata and cuticle protein genes in environmental stresses.

Spiders are representative arthropods of adaptive radiation. The high-quality genomes have only been reported in several web weaver spider species, leaving the wandering spiders' genomic information scarce. The pond wolf spider, Pardosa pseudoannulata, is a representative species in the retrolateral titial apophysis (RTA) clade.

Correlation between carcinoembryonic antigen (CEA) expression and EGFR mutations in non-small-cell lung cancer: a meta-analysis.

Objectives: The purpose of this meta-analysis was to investigate the relationship between serum carcinoembryonic antigen (CEA) expression and epidermal growth factor receptor (EGFR) mutation status in non-small cell lung cancer (NSCLC).

Methods: Databases such as PubMed, Cochrane, EMBASE and Google Scholar were systematically searched to identify studies assessing the association of serum CEA expression with EGFR mutations. Across 19 studies, 4168 patients were included between CEA expression and EGFR mutations odds ratio (OR) conjoint analysis of correlations.