Fndc5/irisin mediates the benefits of aerobic exercise intervention on aging-associated sarcopenia in mice.

Methods: In this study, 24-month-old (aged) mice were used as a naturally occurring model of aging-associated sarcopenia, while 2-month-old (young) mice served as the normal control. Six weeks of treadmill training was employed as an aerobic exercise intervention. The mRNA and protein levels of fibronectin type III domain-containing protein 5 (Fndc5/irisin), MuRF1, and Atrogin-1 in gastrocnemius muscles were analyzed using qRT-PCR and Western blot.

Direct Electroreduction of Low-Concentration CO: Progress and Perspective.

The conversion of CO into carbon-based fuels and chemicals via the electrocatalytic CO reduction reaction (CORR) offers an attractive route to reducing the CO emission for carbon neutrality. Currently, high-purity CO gas has been widely used as the feedstock for most of the CORR studies, while CO sources with a typically low concentration impose the extra cost for CO capture and purification steps. The direct utilization of low-concentration CO for the CORR is a promising approach to substantially address this problem.

Association of abdominal aortic calcification with cognitive impairment in peritoneal dialysis patients.

Background: Patients on peritoneal dialysis (PD) frequently have cognitive impairment, which is linked to a poor prognosis. The purpose of this study was to determine whether abdominal aortic calcification (AAC) may have an impact on PD patients' cognitive function.

Methods: In this cross-sectional study of 110 PD patients, cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), and AAC severity was quantified via lateral lumbar radiography (Kauppila method).

Teacher-student relationship, coping style and sports anxiety among middle school students: a situational study based in Shanghai.

Objective: The present research aims to explore the relationship between coping style, teacher-student relationship, and sports anxiety among middle school students.

Methods: A total of 289 Chinese middle school students from three schools in Shanghai participated in the survey.

Results: (1) A negative teacher-student relationship significantly affects sports anxiety, and conversely, sports anxiety significantly impacts the negative teacher-student relationship, demonstrating a strong mutual effect; (2) Both active coping style and negative coping style significantly influence sports anxiety, while sports anxiety also significantly affects active coping style among middle school students.

Serum Olink Targeted Proteomics Identifies IL-17A as a Prospective Inflammatory Marker for the Prediction and Diagnosis of Kawasaki Disease.

Purpose: Kawasaki disease (KD) is an acute febrile vasculitis and the leading cause of acquired heart disease in children. However, early diagnosis of KD remains challenging, and its pathogenic mechanisms are yet to be fully elucidated. This study utilized Olink Targeted Proteomics to analyze serum protein profiles and identify potential early diagnostic biomarkers for patients with KD.

An Intervertebral Disc (IVD) Regeneration Model Using Human Nucleus Pulposus Cells (iHNPCs) and Annulus Fibrosus Cells (iHAFCs).

Intervertebral disc (IVD) degeneration (IVDD), primarily caused by nucleus pulposus (NP) dehydration, leads to low back pain. While current treatments focus on symptom management or surgical intervention, tissue engineering using IVD-derived cells, biofactors, and scaffolds offers a promising regenerative approach. Here, human NP cells (NPCs) and annulus fibrosus cells (AFCs) are immortalized with human telomerase reverse transcriptase (hTERT), generating immortalized NPCs (iHNPCs) and AFCs (iHAFCs).

Ultrasensitive Detection of Extracellular Vesicles Based on Metal-Organic Framework DNA Biobarcodes Triggered G-Quadruplex Coupled with Rolling Circle Amplification Assay.

Extracellular vesicles (EVs), as liquid biopsy markers for accurate tumor diagnosis, are considered to hold great promise. However, effectively isolating and sensitively detecting EVs with convenience still face challenges. Herein, we propose a highly sensitive and specific platform for EV detection by integrating a metal-organic framework (MOF)-based DNA biobarcodes strategy with a rolling circle amplification (RCA)/G-quadruplex system.

[Determination of 17 perfluorinated/polyfluoroalkyl compounds in serum by high-throughput solid-phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry].

Perfluorinated/polyfluoroalkyl compounds (PFASs) are a group of synthetic chemicals. Since the 1940s, PFASs have been widely used in industrial and daily consumer fields. PFASs can enter the human body through various pathways, such as drinking water, food, and air.

Paternal fenvalerate exposure causes depressive-like behaviour by altering Grb10 gene DNA methylation in adolescent offspring.

Fenvalerate, a typical pyrethroid pesticide, is a neurological toxicant. This study aimed to evaluate the effect of paternal exposure to fenvalerate on depressive-like behaviours in adolescent offspring. Depression-like behavior was determined by Sucrose Preference Test (SPT), Tail Suspension Test (TST) and Forced Swimming Test (FST) in adolescent offspring.

Linkage Position-Controlled Synthesis of Diverse Zirconium Metal-Organic Frameworks with Prominent Intrinsic Proton Conductivities.

Herein, by engineering the geometries of the organic linkers, two pyrrolo-pyrrole-based low-symmetry tetracarboxylate linkers (TAPPs) were successfully designed and subsequently used for the construction of two new zirconium-based metal-organic frameworks (Zr-MOFs) ( and ). The reduction of the linker symmetry arises from both the asymmetric pyrrolo-pyrrole core and the integration of both the - and -benzoate coordination groups on the linkers. Both MOFs are composed of 8-connected Zr nodes and 4-connected highly deformed TAPP linkers with the same topology, but distinct linker arrangements can be observed in two structures.

Suspect Screening of Pharmaceuticals and Their Transformation Products (TPs) in Wastewater during COVID-19 Infection Peak: Identification of New TPs and Elevated Risks.

Pharmaceuticals and their transformation products (TPs) in wastewater are emerging contaminants that pose risks to ecosystems and human health. Here, a typical period marked by the easing of the "zero-COVID" policy in December 2022, resulting in unprecedented infections in China, was chosen to illustrate the environmental impact of pharmaceutical usage during the COVID-19 pandemic. A suspect screening workflow was developed to identify pharmaceuticals and transformation products (TPs) in wastewater influent and effluent from a wastewater treatment plant (WWTP) during the peak and postpeak periods of COVID-19, integrating medication recommendations and TPs' prediction.

Efficacy and safety of corticosteroids for stroke and traumatic brain injury: a systematic review and meta-analysis.

Background: Corticosteroids are frequently used in practice to treat patients with neurological disorders. However, its effect for stroke and traumatic brain injury (TBI) remains controversial. This study aimed to systematically review and evaluate efficacy and safety of corticosteroids for the treatment of stroke and TBI.

Prospective cohort study on characteristics, associated factors and short-term prognosis of sleep and circadian rhythm in intensive care unit: protocol for the SYNC study.

Introduction: Acute sleep and circadian rhythm (SCR) disruption can lead to a range of negative physical and mental consequences, such as depression, delirium, respiratory dysfunction and increased mortality. In the intensive care unit (ICU), the unique environment can exacerbate disruptions in SCR. Few studies have identified the characteristics of SCR in the ICU, and the roles of patient characteristics, illness and medical interventions in ICU SCR remain unclear.

Spatial interpolation and spatiotemporal scanning analysis of human brucellosis in mainland China from 2012 to 2018.

Despite the considerable efforts made to address the issue of brucellosis worldwide, its prevalence in dairy products continues to be difficult to estimate and represents a significant public health concern globally. The aim of the present study was to gain a better understanding of the epidemiology of this disease in mainland China. We set out to investigate the annual spatial distribution and potential hotspots of the disease.

Attack as Defense: Proactive Adversarial Multi-modal Learning to Evade Retrieval.

With growing concerns about information security, protecting the privacy of user-sensitive data has become crucial. The rapid development of multi-modal retrieval technologies poses new threats, making sensitive data more vulnerable to leakage and malicious mining. To address this, we introduce a Proactive Adversarial Multi-modal Learning (PAML) approach that transforms sensitive data into adversarial counterparts, evading malicious multi-modal retrieval and ensuring privacy.

Inhibition of circALPK2 enhances proliferation and therapeutic potential of human pluripotent stem cell-derived cardiomyocytes in myocardial infarction.

Background: Understanding the mechanisms regulating human cardiomyocyte proliferation holds significant promise for developing effective therapies to enhance cardiac regeneration and repair. This study investigates the role of circALPK2, a circular RNA derived from the back-splicing of the 4th exon of alpha protein kinase 2 (ALPK2), in regulating cardiomyocyte proliferation and its therapeutic efficacy in myocardial infarction (MI) treatment.

Methods: Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were used to assess the expression and function ofcircALPK2.

Gegen Qinlian decoction remodels tumor immune microenvironment and inhibits aerobic glycolysis with the synergistic combination of CPT-11 chemotherapy in colorectal cancer therapy.

Ethnopharmacological Relevance: Although several traditional Chinese medicine formulas have demonstrated remarkable outcomes in suppressing the severe gastrointestinal toxicity induced by irinotecan (CPT-11), few studies have investigated whether enhanced anti-cancer efficacy and reduced intestinal toxicity can be achieved through co-administration. CPT-11, as a first-line drug for treating colorectal cancer, has the side effect of intestinal toxicity. Previous studies have primarily focused on using traditional Chinese medicine to alleviate diarrhea caused by CPT-11.

Nutrient concentration, stoichiometry, and timing of delivery can regulate cyanobacterial dominance and microcystin production in rivers.

Riverine cyanobacterial blooms are increasing worldwide and are driven in large part by eutrophication. Despite substantial data on nutrient/bloom relationships in lakes and reservoirs, our understanding of nutrient mechanisms driving cyanobacterial blooms in rivers remains limited as rivers can have more complex temporal and spatial nutrient delivery. This study investigated how nutrient conditions influence cyanobacterial dominance and microcystin production in river phytoplankton.

PIK3CA gene mutation status associated with poor prognosis of breast cancer: a retrospective cohort study.

Purpose: PIK3CA gene mutations have been identified in various malignancies, but the prevalence of specific mutations and their role in breast cancer development remain uncertain. This study aimed to investigate the clinicopathological significance and prognostic impact of PIK3CA mutations in breast cancer.

Methods: Five common PIK3CA mutations (H1047R and H1047L in exon 20, and E542K, E545K, and E545D in exon 9) were identified in breast cancer patients using amplification refractory mutation system (ARMS) allele-specific PCR.

Antitumor Activity of USP7 Inhibitor GNE-6776 in Non-Small Cell Lung Cancer Involves Regulation of Epithelial-Mesenchymal Transition, Cell Cycle, Wnt/β-Catenin, and PI3K/AKT/mTOR Pathways.

Non-small cell lung cancer (NSCLC) is a major cause of cancer-related deaths worldwide. This study investigated the effects and mechanisms of the USP7 inhibitor GNE-6776 on human NSCLC A549 and H1299 cells, providing insights for anti-NSCLC drug development. USP7 expression was analyzed in lung cancer tissue using data from public databases.

Notch1 siRNA and AMD3100 Ameliorate Metabolic Dysfunction-Associated Steatotic Liver Disease.

: As a key mechanism of metabolic dysfunction-associated steatotic liver disease (MASLD) pathogenesis, inflammation triggered by chronic liver injury and immune cells with macrophages enables MASLD to progress to an advanced stage with irreversible processes such as fibrosis, cell necrosis, and cancer in the liver. The complexity of MASLD, including crosstalk between multiple organs and the liver, makes developing a new drug for MASLD challenging, especially in single-drug therapy. It was reported that upregulation of Notch1 is closely associated with the function of pro-inflammatory macrophages.

Association of the DRD and OXT Genetic Polymorphisms With Schizophrenia in a Chinese Population.

The polymorphism of dopamine receptor (DRD) and oxytocin (OXT) may be associated with schizophrenia. A case-control study of 248 schizophrenia patients and 236 controls was conducted using the Sequenom MassARRAY platform. The results showed that DRD2 rs1800497 was a heterozygote (AG vs.

Discovery of novel inhibitors of dengue viral RNA-dependent RNA polymerase by molecular docking, in vitro assay, DFT, and MD simulations.

Dengue virus (DENV) is a significant significant mosquito-borne pathogen. Its RNA-dependent RNA polymerase (RdRp) plays a crucial role in viral replication, making it a critical target for antiviral drug development. The goal of this study was to identify potential inhibitors of the DENV RdRp through virtual screening and experimental assays.

Cadmium spurred Microcystis aeruginosa to unleash more toxic metabolites.

Cyanobacterial harmful algal blooms (cHABs), normally dominated by Microcystis aeruginosa, pose a threat to aquatic ecosystems due to the release of various harmful metabolites. Cadmium (Cd), a heavy metal commonly found in surface water and sediments, often coincides with cHABs in eutrophic lakes. However, the ecotoxicological effects of Cd on M.