Zhonghua Gan Zang Bing Za Zhi
January 2003
Objective: To construct a subtractive cDNA library of genes transactivated by hepatitis B virus X protein (HBX) using suppression subtractive hybridization (SSH) technique and to clone genes associated with HBX transactivating function.
Methods: The mRNA was isolated from HepG2 cells transfected with pcDNA3.1(-)-X and pcDNA3.