Chemical Composition and Anti-Alzheimer's Disease-Related Activities of a Functional Oil from the Edible Seaweed Hizikia fusiforme.

Cholinergic disorder, oxidative stress, and neuroinflammation play important roles in the pathology of Alzheimer's disease. To explore the healthy potential of the edible seaweed Hizikia fusiforme on this aspect, a functional oil (HFFO) was extracted from this alga and investigated on its constituents by gas chromatography-mass spectrometry (GC/MS) in this study. Its anti-Alzheimer's related bioactivities including acetylcholinesterase (AChE) inhibition, antioxidation, and anti-neuroinflammation were evaluated, traced, and simulated by in vitro and in silico methods.

Codon Optimization Significantly Improves the Expression Level of α -Amylase Gene from Bacillus licheniformis in Pichia pastoris.

α-Amylase as an important industrial enzyme has been widely used in starch processing, detergent, and paper industries. To improve expression efficiency of recombinant α-amylase from Bacillus licheniformis (B. licheniformis), the α-amylase gene from B.

High-level expression of pro-form lipase from Rhizopus oryzae in Pichia pastoris and its purification and characterization.

A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL) was cloned into the pPICZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted in Pichia pastoris with a molecular weight of 35 kDa. The maximum lipase activity of recombinant lipase (rProROL) was 21,000 U/mL, which was obtained in a fed-batch cultivation after 168 h induction with methanol in a 50-L bioreactor.

[The analysis of follow-up results of 612 cases of cholecystolithiasis treated with the minimal invasive operation with gallbladder preserved via choledochoscopy].

Objective: To discuss the feasibility of the operation of minimal invasive with gallbladder preserved via choledochoscopy.

Methods: From February 1992 to June 2006, there were 760 patients who underwent cholecystolithiasis treated with the minimal invasive operation with gallbladder preserved via choledochoscopy, among which there were 428 males and 332 females, aged from 18 to 81 years old. All cases were diagnosed by ultrasonography and their gallbladder functions were proved normal by the examination of oral cholecystography or ECT before operation.

[Application of 64-slice helical CT in screening of coronary artery heart disease among apparently healthy people: preliminary experience].

Objective: To evaluate the value of 64-slice helical CT in screening of coronary artery heart disease among apparently healthy people and to explore the specific operation thereof.

Methods: Toshiba Aquilino 64-slice helical CT machine was used to scan the scope from the tracheal carina to cardio-diaphragmatic plain among 286 apparently healthy persons aged 40 approximately 78, 188 males and 98 females, with at least one risk factor of coronary artery heart disease, after the contrast Iopamidol was injected. The data thus obtained underwent after-finishing screen are with the techniques of volume reconstruction, multiplanar reconstruction, curve planar reconstruction, and 3-D reconstruction so as to demonstrate the abnormalities of coronary artery.

Genetic polymorphisms of N-acetyltransferase 2 and colorectal cancer risk.

Aim: To identify the distribution of N-acetyltrasferase 2(NAT2) polymorphism in Hebei Han Chinese and the effects of the polymorphism on the development of colorectal cancer.

Methods: We performed a hospital-based case-control study of 237 healthy individuals and 83 colorectal cancer patients of Hebei Han Chinese. DNA was extracted from peripheral blood and cancer tissues.