Biochar-mediated remediation of low-density polyethylene microplastic-polluted soil-plant systems: Role of phosphorus and protist community responses.

While the prevalent utilization of plastic products has enabled social advancement, the concomitant microplastics (MPs) pollution presents a serious threat to environmental security and public health. Protists, as regulators of soil microorganisms, are also capable of responding most rapidly to changes in the soil environment. The amelioration mechanisms of biochar in the soil-plant systems polluted by low-density polyethylene microplastics (LDPE-MPs) and the response of protist communities in the soil-plant systems polluted by MPs remain unclear.

The MYB-bHLH-NRAMP module modulates the cadmium sensitivity of quinoa by regulating cadmium transport and absorption.

Cadmium (Cd) is one of the most dangerous environmental pollutants and is easily absorbed by food crops. Quinoa is a kind of coarse grain crop with rich nutrition and strong stress resistance, which is easy to accumulate Cd. The increasingly serious soil Cd pollution poses a serious threat to the food safety of quinoa.

The mechanism of alkali to inhibit the organics polymerization in improving the biodegradability of wastewater treated by heat/peroxydisulfate.

High-temperature wastewaters can themselves activate peroxydisulfate (PDS) to remove aromatic contaminants via polymerization. This, however, may result in an insufficient carbon source for denitrification during biochemical treatment, and the formed polymers, without a proper reuse method, will be costly to handle as hazardous waste. This study demonstrates that the addition of NaOH can suppress the polymerization of aromatic contaminants, which is observed not only in simulated wastewater but also in actual coking wastewater (ACW).

Generation of a KDM5D knockout human embryonic stem cell line with CRISPR/Cas9 technology.

KDM5D is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a KDM5D knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D's function in spermatogenesis and its influence on sex differences in various diseases.

Succinic acid synthesis regulated by succinyl-coenzyme A ligase (SUCLA) plays an important role in root response to alkaline salt stress in Leymus chinensis.

Alkaline salts have more severe adverse effects on plant growth and development than neutral salts do. However, the adaptive mechanisms of plants to alkaline salt stress remain poorly understood, especially at the molecular level. The Songnen Plain in northeast China is composed of typical 'soda' saline-alkali soil, with NaHCO and NaCO as the predominant alkaline salts (pH ≥ 9.

Self-assembled doxorubicin prodrug riding on the albumin express train enable tumor targeting and bio-activation.

Doxorubicin (DOX) is a vital anthracycline chemotherapeutic drug, yet presenting significant challenges due to its severe cardiotoxicity. While Doxil enhances the pharmacokinetics and reduces the cardiotoxicity of DOX solution (DOX sol), it shows limitations of low drug loading capacity and inadequate cellular uptake. To overcome these issues, this study developed a novel disulfide bond-linked DOX-maleimide prodrug (DSSM).

Canthaxanthin ameliorates atopic dermatitis in mice by suppressing Th2 immune response.

Atopic dermatitis (AD) is a prevalent chronic inflammatory skin disorder characterized by intense pruritus and complex immunopathogenic mechanisms. Recent evidence has highlighted the critical link between dysregulated intestinal microecology and altered immune responses in AD progression. As essential components of the intestinal microenvironment, metabolites play pivotal roles in various physiological processes.

Brucella abortus transcriptional regulator ArsR6 inhibits host pyroptosis via BAB_RS28760 by triggering the endoplasmic reticulum stress pathway.

Pyroptosis, which is accompanied by inflammatory responses, is critical for pathogen clearance. However, the mechanism through which Brucella evades host pyroptosis remains unclear. The transcriptional regulator ArsR6 maintains bacterial intracellular homeostasis and possibly influences host cell death.

Photoheterotrophic extracellular reduction of ferrihydrite activates diverse intracellular metabolic pathways in Rhodopseudomonas palustris for enhanced antibiotic degradation.

Anoxygenic photosynthetic bacteria (APB) have been frequently detected as a photoautotrophic Fe-carbon cycling drivers in photic and anoxic environment. However, the potential capacity of these bacteria for photoheterotrophic extracellular reduction of iron-containing minerals and their impact on the transformation of organic pollutants remain currently unknown. This study investigated the capacity of R.

Intervention effects of Er Miao san on metabolic syndrome in Bama miniature pigs.

Background: Metabolic syndrome (MS) refers to a cluster of metabolic disorders characterized by systemic chronic inflammation. Er Miao San (EMS) is a classic traditional Chinese medicine compound containing Phellodendron amurense and Atractylodis rhizome at a ratio of 1:1, proven to be effective against inflammatory diseases in clinical practice. Nevertheless, the precise functions of EMS in treating MS and its underlying mechanism have yet to be elucidated.

GelMA@APPA microspheres promote chondrocyte regeneration and alleviate osteoarthritis via Fgfr2 activation.

Background: In the context of osteoarthritis (OA), a condition marked by joint degeneration, there is a notable absence of efficacious approaches to promote regenerative healing in chondrocytes. Novel therapeutic strategies like nanomicelles-hydrogel microspheres loaded with Astragalus polysaccharide (GelMA@APPA) offer promising avenues for promoting chondrocyte regeneration and mitigating OA progression.

Methods: Astragalus polysaccharide (APS) has been shown to induce chondrocyte proliferation and promote cartilage matrix secretion, demonstrating biological activity associated with chondrocyte regeneration.

Heavy metal(loid)s accumulation and human health risk assessment in wheat after long-term application of various urban and rural organic fertilizers.

Composting urban and rural wastes into organic fertilizers for land application is considered the best way to dispose of and recycle waste resources. However, there are some concerns about the long-term effects of applying various organic fertilizers on soils, food safety, and health risks derived from heavy metal(loid)s (HMs). A long-term field experiment was conducted to evaluate the effects of continuous application of chicken manure compost (CM), sewage sludge compost (SSC), and domestic waste compost (DWC) for wheat on the accumulation, transfer, and health risks of HMs.

NR2E3-Associated Retinopathy Presenting with Bilateral Choroidal Neovascularization.

Purpose: We describe an atypical presentation of an 11-year-old female with enhanced S-cone syndrome (ESCS).

Methods: Case report. The patient underwent a thorough ophthalmic examination and investigations such as colour fundus photography, optical coherence tomography, fundus autofluorescence, fluorescein and indocyanine angiography, an electroretinogram and genetic testing.

Noninvasive Assessment of Vascular Endothelial Growth Factor and Prognosis in Gastric Cancer Through Radiomic Features.

Introduction: Gastric cancer (GC) is a leading cause of cancer-related deaths worldwide, with delayed diagnosis often limiting effective treatment options. This study introduces a novel, noninvasive radiomics-based approach using [18F] FDG PET/CT (fluorodeoxyglucose positron emission tomography/computed tomography) to predict vascular endothelial growth factor (VEGF) status and survival in patients with GC. The ability to noninvasively assess these parameters can significantly influence therapeutic decisions and outcomes.

Ultrahigh Energy Storage Performance in BiFeO-Based Lead-Free Ceramics via Tuning Structural Homogeneity and Domain Engineering Strategies.

Lead-free ceramic-based dielectric capacitors are critical in electronics and environmental safety. Nevertheless, developing ideal lead-free ceramics with excellent energy storage properties remains a challenging task for practical applications. Herein, the enhanced relaxation behavior and increased breakdown electric field are utilized to realize the high energy storage behavior of (0.

Bile acid synthesis impedes tumor-specific T cell responses during liver cancer.

The metabolic landscape of cancer greatly influences antitumor immunity, yet it remains unclear how organ-specific metabolites in the tumor microenvironment influence immunosurveillance. We found that accumulation of primary conjugated and secondary bile acids (BAs) are metabolic features of human hepatocellular carcinoma and experimental liver cancer models. Inhibiting conjugated BA synthesis in hepatocytes through deletion of the BA-conjugating enzyme bile acid-CoA:amino acid -acyltransferase (BAAT) enhanced tumor-specific T cell responses, reduced tumor growth, and sensitized tumors to anti-programmed cell death protein 1 (anti-PD-1) immunotherapy.

Digital technology innovation, supply chain resilience and enterprise performance-The case of listed automotive parts manufacturing companies.

Digital technology innovation (DTI) is the core driving force for the development of the digital economy. This paper brings digital technology innovation and the supply chain of auto parts manufacturing under the same framework. This paper uses Stata 18 to empirically analyze the panel data of 130 A-share auto parts listed companies in Shanghai and Shenzhen from 2010 to 2022.

Effect of proprioceptive neuromuscular facilitation on patients with chronic ankle instability: A systematic review and meta-analysis.

Objective: This study conducts a rigorous meta-analysis of existing literature to rigorously examine the efficacy of Proprioceptive Neuromuscular Facilitation (PNF) in ameliorating functional deficits associated with Chronic Ankle Instability (CAI).

Methods: Literature searches were conducted in multiple databases including China National Knowledge Infrastructure (CNKI), VIP, Wanfang, China Biology Medicine disc (CBM), PubMed, EBSCO (Medline, CINAHL, SPORTDiscus, and Rehabilitation & Sports Medicine Source), Embase, ScienceDirect, ProQuest, Cochrane Library, and Web of Science for randomized controlled trials assessing the effects of Proprioceptive Neuromuscular Facilitation interventions on patients with Chronic Ankle Instability. The publication timeframe spanned from the inception of each database until April 10, 2024.

Characterization of Key Lipid Components in the Cell Membrane of Freeze-Drying Resistant Strains Using Nontargeted Lipidomics.

Lactic acid bacteria (LAB) are usually freeze-dried into powder for transportation and storage, with the bacterial membrane playing a crucial role in this process. However, different strains exhibit different levels of freeze-drying resistance in their cell membranes. In this study, () strains 1F20, K56, and J5, demonstrating survival rates of 59.

Genome-Wide A → G and C → T Mutations Induced by Functional TadA Variants in .

The fusion expression of deoxyribonucleic acid (DNA) replication-related proteins with nucleotide deaminase enzymes promotes random mutations in bacterial genomes, thereby increasing genetic diversity among the population. Most previous studies have focused on cytosine deaminase, which produces only C → T mutations, significantly limiting the variety of mutation types. In this study, we developed a fusion expression system by combining DnaG (RNA primase) with adenine deaminase TadA-8e (DnaG-TadA) in , which is capable of rapidly introducing A → G mutations into the genome, resulting in a 664-fold increase in terms of mutation rate.

Protein alterations in patients with delirium after cardiac surgery: An exploratory case-control sub-study of the VISION Cardiac Surgery Biobank.

Background: Delirium is an acute state of confusion associated with adverse postoperative outcomes. Delirium is diagnosed clinically using screening tools; most cases go undetected. Identifying a delirium biomarker would allow for accurate diagnosis, application of therapies, and insight into causal pathways.

CryoEM structure of an MHC-I/TAPBPR peptide-bound intermediate reveals the mechanism of antigen proofreading.

Class I major histocompatibility complex (MHC-I) proteins play a pivotal role in adaptive immunity by displaying epitopic peptides to CD8+ T cells. The chaperones tapasin and TAPBPR promote the selection of immunogenic antigens from a large pool of intracellular peptides. Interactions of chaperoned MHC-I molecules with incoming peptides are transient in nature, and as a result, the precise antigen proofreading mechanism remains elusive.

Mesenchymal stem cell-derived exosomes improved septic lung injury by reducing excessive NETs formation and alleviating inflammatory response.

To illustrate the potential of mesenchymal stem cell-derived exosomes (MSC-Exos) in mitigating septic lung injury by reducing the excessive formation of neutrophil extracellular traps (NETs), a mouse model of septic lung injury was induced through cecal ligation and puncture (CLP). The mice received intraperitoneal injections of MSC-Exos. Post injection, pathological alterations of the lung tissue were evaluated through HE staining, and the levels of inflammatory markers in each mouse group at various time points were assessed using ELISA kits.

Signaling Transduction Network Elucidation of ACE 2 Regulating Autolysis by Using Integrative TMT Proteomics and Transcriptomics.

This study aims to reveal the transduction signaling network that triggers sea cucumber () autolysis. The tandem mass tag (TMT) proteomics and transcriptomic techniques were used to analyze expression differences between inhibited and activated sea cucumber autolysis. Flow cytometry was used to identify apoptosis.