DHI Increases the Proliferation and Migration of Schwann Cells Through the PI3K/AKT Pathway and the Expression of CXCL12 and GDNF to Promote Facial Nerve Function Repair.

The facial nerve is one of the vulnerable nerves in otolaryngology. Repair and recovery of facial nerve injury have a high priority in clinical practice. The proliferation and migration of Schwann cells are considered of great significance in the process of nerve injury repair.

Streptomycin ototoxicity in rat cochlear organotypic cultures.

The purpose of this study is to investigate the ototoxic effect of streptomycin on cochlear hair cells, spiral ganglion cells, and nerve fibers in cochlear organ cultures. The cochlear basilar membrane of three- or four-day-old F344 rats was cultured and treated with various doses of streptomycin for 24 h. Cochlear hair cells and the spiral ganglion neurons were stained with immunofluorescence and were observed under confocal microscope.

[Streptomycin-induced apoptosis of rat cochlear hair cell cultured in vitro].

Objective: To evaluate if caspase pathway was involved in streptomycin-induced cell apoptosis in cochlear hair cells.

Methods: F344 rats at postnatal day 3 or 4 were used for the study in cochlear organotypic cultures. The cochlear basilar membrane was micro-dissected out and cultured overnight, and then treated with 1 mmol/L streptomycin for 24 hours.