[Expression and significance of Ang1, Ang2 and receptor Tie2 in hemangioma].

Objective: To investigate the relationship of angiogenesis and the Ang family members/ receptor (Ang/Tie2) in hemangioma.

Methods: Expression of Ang1, Ang2 and the receptor Tie2 was detected with immunohistochemical SP method and RT-PCR method in 17 cases of proliferating hemangioma, 13 involuting cases and 10 cases of normal children skin.

Results: The expression of Ang2 and Tie2 was higher markedly in proliferating hemangiomas than in involuting hemangiomas (P < 0.

[The heat shock protein 70-2 gene +1267(A/G) polymorphism and susceptibility to ankylosing spondylitis in Chinese Han population].

Objective: To investigate the association between the heat shock protein 70-2 gene polymorphism and ankylosing spondylitis (AS).

Methods: The polymorphisms of HSP70-2 gene Pst I 1267 site were analysed in 176 Chinese Han AS patients and 127 healthy controls by PCR and restriction fragment length polymorphisms(RFLP) methods.

Results: In AS patients HSP70-2 genotypes AA, AG and GG were 46.

[Anti-apoptosis effect of VEGF on the human chronic myelocytic leukemia cell line K562].

To explore the effects of vascular endothelial growth factor (VEGF) on the mechanisms of CML pathogenesis, the effect of VEGF on K562 cell apoptosis induced by As(2)O(3) was analyzed through morphologic observation, DNA fragmentation agarose gel electrophoresis and DNA ploidy flow cytometry analysis, and the effect of VEGF on the expression of bcl-X(L), Bax and caspase-3 in K562 cells was determined by Western blot, meanwhile the expression difference between bcl-X(L) and Bax mRNA in above conditions was detected by RT-PCR. The results showed that after VEGF added, the apoptosis of K562 cells reduced, however, there was no significant changes in cell cycle distribution (P > 0.05).

[Relationship between heat shock protein 70-hom gene polymorphism and ankylosing spondylitis].

Objective: To investigate the association between heat shock protein 70-hom (HSP70-hom) gene polymorphism and ankylosing spondylitis(AS) in Chinese Han patients.

Methods: Genomic DNA from 98 Chinese AS patients and 70 ethnically matched controls were typed for HSP70-hom polymorphism by polymerase chain reaction-restriction fragment length polymorphism.

Results: The HSP70-hom genotypes in the AS patients consisted of homozygote AA (60.

[Relationship between genetic polymorphisms of N-acetyltransferase and susceptibility to hepatocellular carcinoma].

Objective: To study the possible relationship between genetic polymorphism of N-acetyltransferase 2 (NAT2) and susceptibility to hepatocellular carcinoma.

Methods: Genetic polymorphisms of the four NAT2 genes in 78 patients with hepatocellular carcinoma and 112 healthy controls were analyzed by means of real-time fluorescence light-Cycler. The difference in frequencies between the hepatocellular carcinoma patients and the controls were compared.