Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1.

Background: Expression of the full-length isoform of Abelson interactor 1 (ABI1), ABI1-p65, is increased in colorectal carcinoma (CRC) and is thought to be involved in one or more steps leading to tumor progression or metastasis. The ABI1 splice isoform-L (ABI1-SiL) has conserved WAVE2-binding and SH3 domains, lacks the homeo-domain homologous region, and is missing the majority of PxxP- and Pro-rich domains found in full-length ABI1-p65. Thus, ABI1-SiL domain structure suggests that the protein may regulate CRC cell morphology, adhesion, migration, and metastasis interactions with the WAVE2 complex pathway.

Correlation between receptor-interacting protein 140 expression and directed differentiation of human embryonic stem cells into neural stem cells.

Overexpression of receptor-interacting protein 140 (RIP140) promotes neuronal differentiation of N2a cells extracellular regulated kinase 1/2 (ERK1/2) signaling. However, involvement of RIP140 in human neural differentiation remains unclear. We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells.

[Effects of receptor interacting protein 140 on the biological activity of glia cells].

Objective: To explore the effects of receptor interacting protein (RIP) 140 gene overexpression upon the in vitro proliferation, apoptosis, invasion and migration of microglioma cells.

Methods: The BV-2 RIP140 overexpression model (BV-2-1) was constructed by Lipofection and G418 selection, then validated by real-time PCR and Western blotting. The proliferation, apoptosis, invasion and migration potencies were compared between BV-2-1 and its parents by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay, flow cytometry and Transwell chamber.

Overexpression or knock-down of runt-related transcription factor 1 affects BCR-ABL-induced proliferation and migration in vitro and leukemogenesis in vivo in mice.

Background: Runt-related transcription factor 1 (Runx1) plays a crucial role in hematogenesis and its dysfunction may contribute to leukemogenesis. However, it is not clear whether or not abnormal expression of Runx1 will induce leukemia and how the change of Runx1 expression level could affect BCR-ABL-induced leukemogenesis. In the present study, we aimed to analyze if abnormal expression of Runx1 in BaF3 cells alone would induce leukemogenesis.

[Effects of receptor-interacting protein 140 gene knockdown on the proliferation of microglioma cells: experiment with rat microglioma cells].

Objective: To investigate the effects of receptor-interacting protein (RIP)140 gene knockdown on the proliferation of microglioma cells.

Methods: Mouse microglioma cells of the line BV-2 were cultured and transfected with 2 kinds of recombinant RIP140-shRNA plasmids (V2MM-71674 and V2MM-71080) or blank plasmid MSCV-EGFP. Real-time PCR and Western blotting were used to detect the mRNA and protein expression of RIP140; and the cell proliferation was detected by MTT assay.

[Expression profile of receptor-interacting protein 140 in the brain: experiment with prenatal and postnatal mice].

Objective: To investigate the expression of receptor-interacting protein of 140,000 (RIP140) in the developmental brain.

Methods: The brain tissues of 15-day-old and 20-day-old fetal Balb/c mice and 1, 7, 14, 28, 42, and 56-day-old postnatal mice were collected. The expression of RIP140 was examined by immunohistochemistry.

[Screening and identification of human chromosome 21 genes resulting in abnormal development of fetal cerebral cortex with Down syndrome].

Objective: To screen the candidate human chromosome 21 (HC21) genes related to mental retardation (MR).

Methods: The expression of 127 known HC21 genes in the cerebral cortex specimen of a DS fetus and the specimen of a non-DS fetus induced due to maternal disease was detected with Affymetrix U133A gene chip. Semi-quantitative RT-PCR and reverse Northern blotting were used to identify the HC21 genes thus screened.

Cystathionine beta synthase participates in murine oocyte maturation mediated by homocysteine.

Our previous study demonstrated that cystathionine beta synthase (CBS) is highly expressed in the cumulus-oocyte complex during ovulation. However, the role of CBS during oocyte maturation remains uncertain. In this study, a small-interfering (si) RNA interference (siRNA) approach was used to investigate the potential role of CBS during oocyte maturation.

Localization of cystathionine beta synthase in mice ovaries and its expression profile during follicular development.

Background: In vitro fertilization (IVF) researches have suggested that cystathionine beta synthase (CBS) is involved in oocyte development. However, little is known about the regional and cellular expression patterns of CBS in the ovary. The purpose of this study was to analyze the localization of CBS in mice ovaries and to investigate the expression profile during follicular development.

[Cloning, identification, and cellular localization of a down-regulated gene fragment related with Down Syndrome].

Objective: To clone a novel gene and explore its expression patterns in tissues and cells, so as to find its role in the process of encephalopathy in DS.

Methods: On the base of our previous microarray's result together with the tissue type, we chose EST AI480014 to carry out RACE, then analyzed its expression profiles in liver, spleen, kidney, heart, brain by multi-tissues Northern blot, after that semi-quantitive RT-PCR was used to reexamine the expression profiles. Furthermore, we used ISH to find whether aim gene expressed in neuroglial cells cultured in vitro.

[Cell-cycle negative regulatory gene ANA is over-expressed in the brain tissues of patients with Down syndrome].

Objective: To investigate the expression level of genes located in chromosome 21 in the brain tissues of Down syndrome(DS).

Methods: An optimized semi-quantitative RT-PCR method was used to evaluate the expression levels of seven genes encoded in chromosome 21 in fetal cortex brain and cerebellum of DS and the control at the end of 20 weeks of gestation. B2M was used as internal reference to normalize cell loss.

[Preliminary exploration of the influence factors of degenerate oligonucleotide primered PCR of genome DNA].

Objective: To explore the influence factors of the degenerate oligonucleotide primered PCR(DOP-PCR).

Methods: Genome DNA template from the mouse single oocyte or liver tissue were used to perform DOP-PCR. DOP-PCR was carried out with templates of different origin, different gradient dilution, with or without low melting point gel purified to wipe off the small fragment that might interfere with the following analysis, and then PCR of gene FTCD and CBS were carried out to evaluate the influence of these factors on the amplification efficiency and specificity.

[The expression profiles of human chromosome 21 orthologues in mouse M II oocyte].

Objective: To analyse the expression profile of orthologues of human chromosome 21 (HC21) in mouse M II oocytes, and to discuss the relationship between this expression profile and early embryonic development and further to find the possible reasons of DS phenotypes genesis.

Methods: cDNA array and Global RT-PCR methods were used to analyse and identify the expression profile of 93 HC21 orthologues in mouse M II oocytes.

Results: 26 of 93 orthologues were proved to be expressed in mouse M II oocytes and these genes were involved in many biological procedure including transcriptional, metabolism, ionic channel, and ubiquitin pathway etc.