17beta-estradiol stimulates proliferation of spermatogonia in experimental cryptorchid mice.

Aim: To investigate whether estrogen stimulates the proliferation of spermatogonia or induces spermatogenesis in cryptorchid mice.

Methods: Mice were surgically rendered cryptorchid, then treated with different doses of 17beta-estradiol (E2) s.c.

[Differential analysis of proteomic profiles between cryptorchid and normal mouse testes].

To screen factors related to spermatogonial stem cell (SSC) proliferation, and to investigate the mechanism of infertility caused by cryptorchidism, ten-day-old Kunming (KM) mice were used and experimental cryptorchidism was conducted. On the 35th day after cryptorchid operation, the left testes were fixed in Bouin's fluid and used for histological analysis. The testes of 45-day-old mice were subjected to the same histological analysis, and it was found that they contained germ cells at every stage of development, from SSCs to sperm, indicating that the animals were fully sexually mature at this age.

[Cloning of Bmi1 cDNA from mouse testis and its expression in E. coli BL21].

Objective: To produce BMI1 polyclonal antibody, mouse Bmi1 cDNA was cloned from mouse testis and expressed in E. coli BL21.

Methods: Bmi1 gene was amplified from mouse testis by RT-PCR and inserted into the prokaryotic expression vector pET-28c(+).