Foxa1 disruption enhances human cell integration in human-mouse interspecies chimeras.

Blastocyst complementation can potentially generate a rodent model with humanized nasopharyngeal epithelium (NE) that supports sustained Epstein-Barr virus (EBV) infection, enabling comprehensive studies of EBV biology in nasopharyngeal carcinoma. However, during this process, the specific gene knockouts required to establish a developmental niche for NE remain unclear. We performed bioinformatics analyses and generated Foxa1 mutant mice to confirm that Foxa1 disruption could potentially create a developmental niche for NE.

Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae.

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a notorious pest in agriculture that has developed resistance to almost all chemical types used for its control. Here, we assembled a chromosome-level genome for the TSSM using Illumina, Nanopore, and Hi-C sequencing technologies. The assembled contigs had a total length of 103.

Descriptions of Three New Species of the Genus Uchida, 1934 (Hymenoptera, Ichneumonidae, Metopiinae), with an Illustrated Identification Key to Extant Species.

The Asian genus Uchida, 1934 is reviewed based on both morphology and DNA barcodes. Ten species are recognized in total, of which three species from Yunnan Province of China are described as new: sp. nov.

Safety and immunogenicity of aerosolised Ad5-nCoV, intramuscular Ad5-nCoV, or inactivated COVID-19 vaccine CoronaVac given as the second booster following three doses of CoronaVac: a multicentre, open-label, phase 4, randomised trial.

Background: Aerosolised Ad5-nCoV is the first approved mucosal respiratory COVID-19 vaccine to be used as a booster after the primary immunisation with COVID-19 vaccines. This study aimed to evaluate the safety and immunogenicity of aerosolised Ad5-nCoV, intramuscular Ad5-nCoV, or inactivated COVID-19 vaccine CoronaVac given as the second booster.

Methods: This is an open-label, parallel-controlled, phase 4 randomised trial enrolling healthy adult participants (≥18 years) who had completed a two-dose primary immunisation and a booster immunisation with inactivated COVID-19 vaccines (CoronaVac only) at least 6 months before, in Lianshui and Donghai counties, Jiangsu Province, China.

Safety and immunogenicity of heterologous boost immunisation with an orally administered aerosolised Ad5-nCoV after two-dose priming with an inactivated SARS-CoV-2 vaccine in Chinese adults: a randomised, open-label, single-centre trial.

Background: Due to waning immunity and protection against infection with SARS-CoV-2, a third dose of a homologous or heterologous COVID-19 vaccine has been proposed by health agencies for individuals who were previously primed with two doses of an inactivated COVID-19 vaccine.

Methods: We did a randomised, open-label, controlled trial to evaluate the safety and immunogenicity of heterologous boost immunisation with an orally administered aerosolised adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in Chinese adults (≥18 years old) who had previously received two doses of an inactivated SARS-CoV-2 vaccine-Sinovac CoronaVac. Eligible participants were randomly assigned (1:1:1) to receive a heterologous booster vaccination with a low dose (1·0 × 10 viral particles per mL; 0·1 mL; low dose group), or a high dose (1·0 × 10 viral particles per mL; 0·2 mL; high dose group) aerosolised Ad5-nCoV, or a homologous intramuscular vaccination with CoronaVac (0·5 mL).

Effective Material Basis and Mechanism Analysis of Compound Banmao Capsule against Tumors Using Integrative Network Pharmacology and Molecular Docking.

Purpose: Compound banmao capsule (CBC), a well-known traditional Chinese medical material, is known to inhibit various tumors. However, its material basis and pharmacological mechanisms remain to be elucidated. This study aimed to investigate the effective material basis and mechanisms of action of CBC against tumors.

Comparative mitogenomics and phylogenetics of the stinging wasps (Hymenoptera: Aculeata).

The stinging wasps (Hymenoptera: Aculeata) include diverse groups such as vespid wasps, ants and bees. Phylogenetic relationships among major lineages of stinging wasps have been inferred from molecular and morphological data. However, the genomic features of the mitochondrial genomes and their phylogenetic utility remain to be explored.

Revision of the genus Oxyscelio Kieffer (Hymenoptera, Scelionidae) from China.

The genus Oxyscelio Kieffer from China is revised. Thirty-four species are recognized, of which two species are described as new: O. nullicarina Mo Chen, sp.

Application of DNA barcoding confirms the host of Olmi, 1986 (Hymenoptera, Dryinidae).

Olmi, 1986 was originally described from Vietnam based on a single female. No further distribution records or hosts have been documented since its original discovery. In the present study, this species is newly recorded from China and its host is confirmed as (Distant, 1918) using DNA barcoding techniques.

A new species of Cylloceria Schiødte, 1838 (Hymenoptera, Ichneumonidae) from the Oriental Region.

A new species of the genus Cylloceria Schiødte, 1838 from Thailand and Myanmar, Cylloceria ebbae sp. nov. is described and illustrated.

Taxonomic study of the genus Ozols (Hymenoptera, Ichneumonidae, Pimplinae) with description of a new species from China and a key to world species.

Five species of the genus Ozols are reported. One, Sheng & Li, collected from Liaoning province, China, is new to science. In addition, digital images and a taxonomic key to the all species of are presented.

An updated checklist of the extant Western Palaearctic Dryininae (Hymenoptera, Dryinidae).

A checklist of 20 extant species of Dryininae (Hymenoptera, Dryinidae) from the Western Palaearctic subregion is presented.

Electroacupuncture stimulates the proliferation and differentiation of endogenous neural stem cells in a rat model of ischemic stroke.

Electroacupuncture (EA) may stimulate neurogenesis in animal models of ischemic stroke; however, the associated mechanisms are not clear. The present study aimed to evaluate the neurogenesis efficacy of EA on ischemic stroke and the underlying associated mechanisms. A model of middle cerebral artery occlusion (MCAO) was employed as the rat model of brain ischemia and reperfusion.

Revision of the Oriental genus Zavatilla Tsuneki (Hymenoptera, Mutillidae, Trogaspidiini), with descriptions of two new species.

Four species of the genus Zavatilla Tsuneki, 1993 are reviewed. An updated diagnosis of the genus is given and two new sex associations are proposed. Two new species, Z.

Contribution to the knowledge on the spider wasps genus Morochares Banks, 1934 (Hymenoptera: Pompilidae) from China.

Five species of the genus Morochares Banks, 1934 are reported from China, four of them are described and illustrated as new species: M. sinica Loktionov Lelej, sp. nov.

A novel three-dimensional tumorsphere culture system for the efficient and low-cost enrichment of cancer stem cells with natural polymers.

Cancer stem cells (CSCs) are considered to serve a key role in tumor progression, recurrence and metastasis. Tumorsphere culture is the most important method for enriching CSCs and is widely used in basic research and drug screening. However, the traditional suspension cell culture system has several disadvantages, including low efficiency, high cost and difficult procedure, making it difficult to produce tumorspheres on a large scale.

Next-Generation Sequencing of Two Mitochondrial Genomes from Family Pompilidae (Hymenoptera: Vespoidea) Reveal Novel Patterns of Gene Arrangement.

Animal mitochondrial genomes have provided large and diverse datasets for evolutionary studies. Here, the first two representative mitochondrial genomes from the family Pompilidae (Hymenoptera: Vespoidea) were determined using next-generation sequencing. The sequenced region of these two mitochondrial genomes from the species sp.

[Immobilization of Estrogen-degrading Bacteria to Remove the 17β-estradiol and Diethylstilbestrol from Polluted Water and Cow Dung].

Estradiol (E2) and diethylstilbestrol (DES) can be enriched in sewage and cow dung, posing serious threats to human and ecological health. Strain Rhodococcus sp. JX-2 and strain Serratia sp.

The mitochondrial genome of the Vespa mandarinia Smith (Hymenoptera: Vespidae: Vespinae) and a phylogenetic analysis of the Vespoidea.

We report the mitochondrial genome of the Vespa mandarinia Smith (GenBank accession no. KR059904) for the subfamily Vespinae (Hymenoptera: Vespidae). This is the first completely sequenced mitochodrial genome from the genus Vespa.

Blockage of protease-activated receptor 1 ameliorates heat-stress induced intestinal high permeability and bacterial translocation.

Accumulated evidences indicate intestinal lesions play an important role in the pathogenesis of heatstroke. However, the underlying mechanisms by which heat stress causes intestinal barrier dysfunction and bacterial translocation remain unclear. In this study, we investigated the role of protease-activated receptor 1 (PAR1) in heat stress-induced intestinal hyper-permeability and bacterial translocation.

The genus Omalus Panzer, 1801 (Hymenoptera, Chrysididae) from China, with descriptions of four new species.

The Chinese species of the genus Omalus Panzer, 1801 are revised and keyed for the first time. Eight species are recorded, of which four are new to science and one is new to China: Omalus aeneus (Fabricius, 1787), Omalus berezovskii (Semenov-Tian-Shanskij, 1932), Omalus potanini (Semenov-Tian-Shanskij, 1932), Omalus imbecillus (Mocsáry, 1889) (new to China), Omalus helanshanus sp. n.

The discovery of genus Fredegunda Fitton, Shaw & Gauld in China, with description of a new species (Hymenoptera, Ichneumonidae, Pimplinae).

The genus Fredegunda Fitton, Shaw & Gauld (Ichneumonidae, Pimplinae) is newly reported both from China and the Oriental region. A new species, Fredegunda sinica sp. nov.

Notes on the genus Ismarus Haliday (Hymenoptera, Diapriidae) from China.

The Chinese species of the genus Ismarus Haliday, 1835, are revised for the first time. Three new species from the Oriental region of China and belonging to Ismarus halidayi-group are described and illustrated: Ismarus longussp. n.