Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples.

Loop-mediated isothermal amplification (LAMP), an attractive DNA amplification method, was developed as a valuable tool for the rapid detection of Toxoplasma gondii. In this study, species-specific LAMP primers were designed by targeting the AF146527 sequence, which was a conserved sequence of 200- to 300-fold repetitive 529 bp fragment of T.gondii.

Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite.

Background: Advancements have been made in the genetic manipulation of apicomplexan parasites. Both the in vitro transient and in vivo stable transfection of Eimeria tenella have been developed successfully. Herein, we report the transient and stable transfection of Eimeria mitis.

[Application of FTIR spectroscopy to the identification of Glycyrrhiza uralensis Fisch].

Fourier transform infrared spectroscopy with a spectra range of 4 000-400 cm(-1) was used to identify fifty nine samples of wild or cultivated Glycyrrhiza uralensis Fisch. from six different places around China. The 1D spectra and their second derivative spectra of herbal samples, the corresponding extract samples and two marker compounds (glycyrrhizic acid and liquiritin) were compared.