Optimization of Fermentation Conditions in Ergothioneine Biosynthesis from Ganoderma resinaceum (Agaricomycetes) and an Evaluation of Their Inhibitory Activity on Xanthine Oxidase.

Ganoderma resinaceum is a traditional mushroom that contains natural products, including ergothioneine (EGT), which has powerful antioxidant properties in the human body. To increase EGT yield from G. resinaceum, the optimal carbon and nitrogen sources in the culture medium were determined as 20 g/L sucrose and 4 g/L NH4Cl, respectively.

Multiplex gene precise editing and large DNA fragment deletion by the CRISPR-Cas9-TRAMA system in edible mushroom Cordyceps militaris.

The medicinal mushroom Cordyceps militaris contains abundant valuable bioactive ingredients that have attracted a great deal of attention in the pharmaceutical and cosmetic industries. However, the development of this valuable mushroom faces the obstacle of lacking powerful genomic engineering tools. Here, by excavating the endogenous tRNA-processed element, introducing the extrachromosomal plasmid and alongside with homologous template, we develop a marker-free CRISPR-Cas9-TRAMA genomic editing system to achieve the multiplex gene precise editing and large synthetic cluster deletion in C.