LPS induces SGPP2 to participate metabolic reprogramming in endothelial cells.

Sepsis often causes organ dysfunction and is manifested in increased endothelial cell permeability in blood vessels. Early-stage inflammation is accompanied by metabolic changes, but it is unclear how the metabolic alterations in the endothelial cells following lipopolysaccharide (LPS) stimulation affect endothelial cell function. In this study, the effects of 1 μg/ml of LPS on the metabolism of human umbilical vein endothelial cells (HUVECs) were investigated, and the metabolic changes after LPS stimulation were explained from the perspective of mRNA expression, chromatin openness and metabolic flux.

[Advances in the research of fluid resuscitation for burn shock].

The aim of fluid resuscitation is to restore tissue perfusion, ameliorate cellular injury, increase tissue perfusion and oxygenation, and improve end-organ functions. Many researches in the field of fluid resuscitation strategy have been made in the last decade, but vigorous debate on optimal method of resuscitation still exists. The hypotensive resuscitation strategy is particularly applicable in patients with uncontrollable hemorrhage, whereas, it is uncertain whether the theory could be used in burn shock management.

[Multi-center clinical trial of FLAMIGEL (hydrogel dressing) for the treatment of residual burn wound].

Objective: To evaluate the effect of FLAMIGEL (hydrogel dressing) on the repair of residual burn wound.

Methods: Sixty burn patients with residual wounds hospitalized in 6 burn units from November 2011 to May 2012 were enrolled in the multi-center, randomized, and self-control clinical trial. Two residual wounds of each patient were divided into groups T (treated with FLAMIGEL) and C (treated with iodophor gauze) according to the random number table.

[Selection of antifungal agents for burn patients].

Fungal infection is one of the serious complications of severely burned patients with high mortality. Application of antifungal agents timely and rationally is very important to control the infection. Antifungal agents including polyenes,triazoles, and echinocandins have been used widely in burned patients and are proved to be effective.

[Effects of topical agents for burns on Acinetobacter baumannii within biofilm].

Objective: To observe the antimicrobial activity of topical agents commonly used for burns on Acinetobacter baumannii (AB) in both free and biofilm states, and their synergistic effect with ambroxol on AB within biofilm.

Methods: Eleven AB strains were isolated from wound excretion, respiratory tract, and blood of patients hospitalized in our hospital from August 2005 to April 2007. (1) The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of mafenide acetate and chlorhexidine acetate to free AB (including drug-resistant, drug-sensitive, and standard strains) were determined by dilution method.

[Changes in expression of gene aba I in biofilm of Acinetobacter baumannii strains isolated from burn patients].

Objective: To study the changes in expression of quorum sensing gene aba I in Acinetobacter baumannii (AB) strains isolated from burn patients during biofilm formation process, and its influences on the extracellular matrix of biofilm and drug resistance of AB.

Methods: Six drug-resistant and five drug-sensitive AB strains isolated from wound excretion, blood and venous catheter were collected from burn patients hospitalized in Ruijin hospital of Shanghai Jiao Tong University School of Medicine from January to October 2011. The AB standard strain ATCC 19606 was used as control.

[Expressions of pgaABC gene clusters and changes in biofilm phenotype of Acinetobacter baumannii in burn patients].

Objective: To observe expressions of pgaABC gene clusters and changes in biofilm (BF) phenotype in Acinetobacter baumannii (AB) isolated from burn patients.

Methods: From January 2009 to October 2010, 24 strains of AB isolated from burn patients hospitalized in our burn wards were collected for the study, while the standard strain ATCC 19606 was used as control. Expressions of pgaABC gene clusters were detected by real time fluorescence quantitative RT-PCR.

[Drug-resistance of Acinetobacter baumannii isolated from burn wards and analysis of homogeneity].

Objective: To monitor genotypes and drug-resistance trend of Acinetobacter baumannii (AB) isolated from burn wards.

Methods: Twenty-six strains of AB isolated from wound secretion, venous catheter, and blood were collected from burn patients hospitalized in our burn wards from November 2008 to February 2009, and June to September 2010. Homogeneous genotype analysis was performed with repetitive extragenic palindromic PCR, and drug-resistance rate to 13 antibiotics including amikacin, gentamicin, etc.

[Drug-resistance Acinetobacter baumannii infection in burn patients: current situation and countermeasure].

Infections caused by Acinetobacter baumannii (AB) have emerged as a knotty clinical problem in the burn wards due to its omni-resistance to antibiotics and high prevalence. Although our knowledge in regard to the pathogenesis and the resistance mechanisms of AB is increasing, the available treatment remains much limited. Measures to effectively control nosocomial infection are warranted.

[Formation of bacterial biofilm on deep vein catheters in burn patients and its significance].

Objective: To observe the biofilm (BF) formation of Staphylococcus aureus (SA), Acinetobacter baumannii (AB) and Pseudomonas aeruginosa (PA) on the surface of deep vein catheters in burn patients after infection.

Methods: The bacteria from deep vein catheters in 20 patients hospitalized from November 2008 to August 2009 were isolated, and were compared with their respective standard stains. Catheters tips were examined with scanning electron microscope (SEM).

[Strategies for prevention and cure of burn infection].

Infection is still the major cause of death in severe burn patients, thus the optimization of antibiotic therapy is an important approach to the annihilation of pathogenic bacteria and the decrease of drug-resistance bacteria. It is urgent for burn surgeons to face the selection pressure of antibiotics and the fungous infections following the incorrect use of antibiotics. Regardless of its complexity, the treatment of sepsis associated with post-burn bacterial infections should be systematical.

[Multi-center clinical study of the effect of silver nitrate ointment on the partial-thickness burn wounds].

Objective: To evaluate the therapeutic effect of silver nitrate ointment on partial-thickness burn wounds, and observe its side-effects.

Methods: Multi-center, randomized, positive drug paralleled self-controlled trial was carried out. Eighty patients with superficial partial-thickness burns, and 40 with deep-partial thickness burns were randomized into AgNO3 group and SD-Ag group according to drug topically applied to the wounds.

[Experimental study of human skin fibroblasts cultured in three-dimension(3D)].

Objective: To investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL).

Methods: The human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology.

Adipocyte-selective reduction of the leptin receptors induced by antisense RNA leads to increased adiposity, dyslipidemia, and insulin resistance.

Although recent evidence suggests that leptin can directly regulate a wide spectrum of peripheral functions, including fat metabolism, genetic examples are still needed to illustrate the physiological significance of direct actions of leptin in a given peripheral tissue. To this end, we used a technical knock-out approach to reduce the expression of leptin receptors specifically in white adipose tissue. The evaluation of leptin receptor reduction in adipocytes was based on real time PCR analysis of the mRNA levels, Western blot analysis of the proteins, and biochemical analysis of leptin signaling capability.

[Clinical observation of the long-term effects of rhEGF on deep partial-thickness burn wounds].

Objective: To evaluate the safety and long-term effect of recombinant human epithelial growth factor (rhEGF) on deep partial-thickness burn wounds.

Methods: Thirty-seven burn patients were enrolled in this study and were observed by randomized, double-blinded and placebo-controlled protocol. An area of deep partial-thickness burn wounds from each patient was divided into control (C) and treatment (T) portions.

[Experimental study on transplantation of acellular xeno-dermis and suspended keratinocytes].

Objective: To investigate the outcome and histological changes of transplantation of acellular xeno-dermis combined with suspended keratinocytes.

Methods: Forty-two nude mice with full-thickness skin defect on the back were randomly divided into 2 groups, then acellular xeno-dermisand and suspended keratinocytes were adopted to cover the skin defect in the experimental group, pure suspended keratinocytes in the control group. The area of wound healing was calculated 2, 3 and 5 weeks after transplantation, and the rates of wound contraction were also calculated, and biopsy for histological examination was performed 3, 6 and 12 weeks after transplantation.