[Early reconstruction of the periorbital injuries].

Objective: To study the clinical characteristics and the treatment of periorbital injuries.

Methods: 61 cases were treated, including 30 cases orbitozygomatic fracture, 6 cases of frontal-orbital fracture, 8 cases of naso-ethmoid-orbital fracture, 7 cases of blow -out fracture and 10 cases of complicated fracture. The patients were diagnosed after physical examination and other examination, like CT.

[Repair of occipital and nuchal wounds with inferior trapezius myocutaneous flaps in patients after high voltage electrical burn].

Objective: To explore the methods and effects of repair of occipital and nuchal wounds with inferior trapezius myocutaneous flap after deep electrical bum.

Methods: Twelve patients with high-voltage electrical burn in occipital and nuchal regions were hospitalized to our ward from March 2003 to September 2007. They were repaired with improved inferior trapezius myocutaneous flaps after debridement.

Influence of CD14 polymorphism on CD14 expression in patients with extensive burns.

We sought to investigate the association of CD14 genotype with the risk of mortality after burn, and we also attempted to evaluate whether CD14-159 C/T polymorphism affects the kinetics and extent of CD14 expression as well as its release, and TNF-alpha expression in burned patients. The study involved 64 patients in Chinese Han population incurring burns covering more than 30% of the total body surface area. CD14 polymorphism was determined by polymerase chain reaction (PCR) and subsequent restriction fragment length polymorphism (RFLP) analysis.

[The influence of CD14 genomic polymorphism on CD14 gene expression as well as protein release and its clinical significance in patients with extensive burns].

Objective: To investigate the influence of a lipopolysaccharide receptor CD14-159C/T genomic polymorphism on CD14 gene expression as well as protein release, and the relation of sepsis susceptibility and prognosis in patients with extensive burns.

Methods: The study group consisted of 26 patients with burns covering more than 30% of the total body surface area. The CD14 gene polymorphism was determined by polymerase chain reaction (PCR) and subsequent HaeIII restriction enzyme digestion of the PCR products.

[Association between a genomic polymorphism within the CD14 locus and severe sepsis susceptibility as well as prognosis in patients after extensive burns].

Objective: To investigate the relation of a lipopolysaccharide receptor CD14C-159T gene polymorphism to severe sepsis susceptibility and prognosis in patients with extensive burns.

Methods: The study group consisted of 118 normal controls and 16 patients with burns covering more than 60 percent total body surface area. Typing of each patient for the CD14C-159T gene polymorphism was performed by analyzing restriction fragments of a Hae III-digested DNA fragment obtained using polymerase chain reaction (PCR-RFLP).

[Changes in the percentage of human leukocyte antigen DR in CD(+)(14) monocytes in burned patients].

Objective: To observe the changes in the percentage of CD(+)(14) monocytes expressing human leukocyte antigen DR(HLA-DR) in burn patients.

Methods: Thirty burn patients were divided into three groups according to body surface area burned: severe burn group [>60% total body surface area(TBSA)]; moderate burn group (25%-30% TBSA), mild burn group(<10% TBSA). Blood samples were collected on 3, 7, 14, 28 and 49 postburn days(PBD), and CD(+)(14) monocyte HLA-DR expression was determined with monoclonal antibody staining and flow cytometry.