Comparative metabolism of cinobufagin in liver microsomes from mouse, rat, dog, minipig, monkey, and human.

Cinobufagin (CB), a major bioactive component of the traditional Chinese medicine Chansu, has been reported to have potent antitumor activity. In this study, in vitro metabolism of CB among species was compared with respect to metabolic profiles, enzymes involved, and catalytic efficiency by using liver microsomes from human (HLM), mouse (MLM), rat (RLM), dog (DLM), minipig (PLM), and monkey (CyLM). Significant species differences in CB metabolism were revealed.

[Effects of precursor feeding and fungal elicitors on secondary metabolits in cell suspension culture of Cistanche deserticola].

Objective: To investigate the effects of precursor feeding and fungal elicitors on cell growth and the contents of PeGs and Echin in the cell supension culture of Cistanche deserticola.

Methods: The combination of precursor feeding and fungal elicitors were added on the cell suspension culture.

Results: The combination of L-phenylalanine and L-Tyrosing were added with Cladosporium fulvum, the dry weight of cell and the contents of PeGs and Echin in suspention all reached the highest value, 14.