Development of a simplified approach for the fabrication of localised surface plasmon resonance sensors based on gold nanorods functionalized using mixed polyethylene glycol layers.

A simplified approach for the fabrication of localised surface plasmon resonance (LSPR) sensors based on gold nanorods (GNRs) is described and validated in a model immunoassay for the activated leukocyte cell adhesion molecule (ALCAM) cancer biomarker. Towards improving on standard bottom-up LSPR sensor fabrication methodologies, we demonstrate that GNRs bioconjugated with monoclonal antibodies can be readily covalently immobilized onto silanized glass substrates to yield highly sensitive LSPR sensors. To maximise the performance of the proposed sensors, mixed polyethylene glycol adlayers were optimized in regards to the bioconjugation of monoclonal antibodies using the standard carbodiimide chemistry.

Complementary metal oxide semiconductor compatible silicon nanowires-on-a-chip: fabrication and preclinical validation for the detection of a cancer prognostic protein marker in serum.

An integrated translational biosensing technology based on arrays of silicon nanowire field-effect transistors (SiNW FETs) is described and has been preclinically validated for the ultrasensitive detection of the cancer biomarker ALCAM in serum. High-quality SiNW arrays have been rationally designed toward their implementation as molecular biosensors. The FET sensing platform has been fabricated using a complementary metal oxide semiconductor (CMOS)-compatible process.