Publications by authors named "Jing-Guo Wu"
Article Synopsis
- - This study investigated how silencing the TLR4 gene affects adipocytokine expressions in a rat model of obstructive sleep apnea hypopnea syndrome (OSAS) combined with hypertension.
- - Researchers successfully established the OSAS with hypertension model and observed significant physiological changes and increased blood pressure in the affected rats compared to controls.
- - The findings revealed that silencing TLR4 through RNA interference significantly lowered inflammatory cytokines and adipocytokines in the rats, suggesting its potential role in regulating OSAS with hypertension.
Article Synopsis
- Macrophage polarization can adapt based on various signaling pathways and transcription factors, with SOCS acting as a crucial inhibitor in immune responses.
- Transfection of SOCS1 shRNA in mouse macrophages led to increased levels of JAK1 and STAT1, suggesting enhanced signaling through the JAK1/STAT1 pathway.
- Fludarabine treatment affected the expression of pro-inflammatory cytokines, altering M1 and M2 macrophage polarization dynamics, indicating the complex interplay between SOCS1 and macrophage activation.
Sepsis is one of the most challenging health problems worldwide. Our previous study showed that chronic schistosoma japonica (SJ) infection might increase serum anti-inflammatory factors to play a protective role, thus improving the survival rate of septic mice. Further research revealed that SJ infection promoted J774A.
View Article and Find Full Text PDFObjective: This paper aimed to investigate ego modules for TGFβ3-induced chondrogenesis in mesenchymal stem cells (MSCs) using ego network algorithm.
Methods: The ego network algorithm comprised three parts, extracting differential expression network (DEN) based on gene expression data and protein-protein interaction (PPI) data; exploring ego genes by reweighting DEN; and searching ego modules by ego gene expansions. Subsequently, permutation test was carried out to evaluate the statistical significance of the ego modules.
Article Synopsis
- *The study found that a specific protein from Trichinella spiralis, called rTsP53, has anti-inflammatory effects that can protect mice from a model of sepsis induced by LPS.
- *rTsP53 treatment lowered harmful inflammatory cytokines and promoted the activation of M2 macrophages, suggesting it helps modulate the immune response to prevent severe sepsis.
Article Synopsis
- Micrometam C is a new marine compound derived from the mangrove plant Micromelum falcatum, which was studied for its anti-inflammatory effects.
- It showed a significant reduction in immune cell migration during inflammation in genetically modified zebrafish and decreased harmful reactive oxygen species (ROS) in both zebrafish and macrophage cells.
- The compound also helped restore key antioxidant levels and inhibited specific inflammatory pathways, suggesting that its protective effects are largely due to its antioxidant properties.
Allogeneic bone marrow mesenchymal stem cell (allo-BMSC)-based tissue-engineered bone (TEB) has great potential for bone defect repair. However, the immunogenicities and biological roles of allo-BMSCs are still controversial. In this study, we established an animal model of critical-sized mandibular defect in beagle dogs and compared the repairing effects of allo-BMSC-based TEB with autogenic BMSC (auto-BMSC)-based TEB without the administration of immunosuppressants.
View Article and Find Full Text PDF[The research of establishing discriminant function for patients with angina pectoris by stepwise analysis based on serum inflammatory factors].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
December 2012
Objective: To improve cost-efficiency, discriminant functions in stepwise method was founded for the differential diagnosis of angina pectoris by detecting the serum level of high-sensitivity C-reactive protein (hs-CRP), macrophage migration inhibitory factor (MIF), interleukin-4 (IL-4) and interleukin-10 (IL-10) in patients with stable angina pectoris (SAP) and unstable angina pectoris (UAP).
Methods: Thirty-nine SAP patients and 47 UAP patients were enrolled into the study, while 39 healthy volunteers were enrolled into the controlled group forming the entire set of training samples. The serum levels of hs-CRP, MIF, IL-4 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA).
[Expression and effects of microRNA-155 in the livers of septic mice].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
March 2012
Objective: To evaluate the effects of microRNA-155 (miR-155) on liver injury in mice with sepsis.
Methods: One hundred and twenty BALB/c mice were randomly divided into two groups of equal number according to random number table. Sepsis was induced by intraperitoneal injection of lipopolysaccharide (LPS,20 mg/kg).
[The protective effect of chronic schistosoma japonica infestation against sepsis in mice].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
May 2011
Objective: To preliminarily study the protective effect of chronic schistosoma japonica (SJ) infestation against sepsis in mice and its mechanism.
Methods: BALB/c male mice were used, and the experiment was divided into three parts. Experiment 1: chronic SJ infestation model was reproduced by SJ cercaria inoculation through abdominal skin for 8 weeks.
[Polymorphism and structural analysis of suppressor of cytokine signaling-1 of rat].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
May 2010
Objective: To identify the suppressor of cytokine signaling-1 (SOCS-1) of rat from the amplified gene with the help of bioinformatics to predict the deduced protein's structure and function in order to lay the foundation for further theoretical study.
Methods: The full-length rat SOCS-1 gene was amplified and identified from the GeneBank Nucleotide database, and the corresponding structure and function of its deduced protein was predicted by the bioinformatics analyzing tools online and the complicated bioinformatics software package Vector NTI suite 8.0, meanwhile the molecular cladogram was reconstructed.
Article Synopsis
- Rosiglitazone, commonly used to treat Type 2 diabetes, has been found to provide cardioprotection against ischemia-reperfusion (I/R) injury, though the exact mechanisms were previously unclear.
- A study showed that mice pretreated with rosiglitazone exhibited less heart damage and reduced cardiac cell death after I/R, and the phosphatidylinositol 3-kinase (PI3-K)/Akt signaling pathway played a crucial role in this protective effect.
- Inhibition of the PI3-K pathway using wortmannin negated the cardioprotective benefits of rosiglitazone, highlighting its importance as a potential therapeutic target for reducing myocardial I/R injury.