Publications by authors named "Jing-Fu Wu"
The aim of this study was to understand the enterovirus types and biological features of pediatric cases of HFMD in Sanmenxia City during 2011, and compare the latter to a cohort of healthy children. Stool samples of 55 cases of HFMD and 60 healthy children were collected for the isolation and identification of enteroviruses using RNA extraction and real-time RT-PCR assays. EV71 and CA16 were identified by nucleotide sequencing using virus-specific VP1 primers; for the other enteroviruses, 012/011 and 008/013 primers were used for amplification and sequencing.
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- The study aimed to analyze the genomic characteristics of the coxsackievirus A16 (CoxA16) strain linked to hand-foot-mouth disease in Henan province, using 406 samples processed through RT-PCR and cell culture methods.
- The entire genome of the CoxA16 isolate was sequenced, revealing a length of 7411 base pairs, and showed varying nucleotide similarities with other CoxA16 strains in specific genetic regions.
- Phylogenetic analysis indicated that this strain belongs to the subgenotype C2/B-2 and clustered with strains from other locations, emphasizing its relevance for monitoring and controlling HFMD outbreaks.