ZIF-8/nitrogen-doped reduced graphene oxide as thin film microextraction adsorbents for simultaneous determination of novel halogenated flame retardants in crayfish-aquaculture water systems.

Novel halogenated flame retardants (HFRs) have attracted much attention due to their environmental hazard and adverse effects on human health. In this study, a sensitive and simultaneous method for the determination of six novel HFRs was developed, including tetrabromobisphenol A (TBBPA), tetrachlorobisphenolA, TBBPA bis(2-hydroxyethyl ether), TBBPA bis(allyl ether), TBBPA bis(2,3-dibromopropyl ether) and 2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine. ZIF-8 modified nitrogen-doped reduced graphene oxide (ZIF-8@N-rGO) was synthesized and coated onto a syringe filter to prepare a thin film microextraction (TFME) device.

Combined effects of progesterone and SOCS3 DNA methylation on T2DM: a case-control study.

Background: This study aims to investigate the independent and combined effects of progesterone and suppressor of cytokine signaling (SOCS)-3 DNA methylation on type 2 diabetes mellitus (T2DM) among men and postmenopausal women in rural China.

Methods: A case-control study with 914 participants (329 T2DM, 585 controls) was conducted. Serum progesterone was detected with liquid chromatography-tandem mass spectrometry.

miR‑383 increases the cisplatin sensitivity of lung adenocarcinoma cells through inhibition of the RBM24‑mediated NF‑κB signaling pathway.

The expression of microRNA‑383 (miR‑383) is downregulated in a variety of tumor tissues, and it exhibits antiproliferative activity in non‑small cell lung cancer cells. In the present study, an association between the downregulation of miR‑383 expression and the deletion of chr8p22 in patients with lung adenocarcinoma was identified. The promoting effect of miR‑383 on cisplatin sensitivity was verified both and .

Towards Fair Knowledge Transfer for Imbalanced Domain Adaptation.

Domain adaptation (DA) becomes an up-and-coming technique to address the insufficient or no annotation issue by exploiting external source knowledge. Existing DA algorithms mainly focus on practical knowledge transfer through domain alignment. Unfortunately, they ignore the fairness issue when the auxiliary source is extremely imbalanced across different categories, which results in severe under-presented knowledge adaptation of minority source set.

Using a reactive emulsifier to construct simple and convenient nanocapsules loaded with lambda-cyhalothrin to achieve efficient foliar delivery and insecticidal synergies.

Nanocapsules are a promising controlled release formulation for foliar pest control. However, the complicated process and high cost limit widespread use in agriculture, so a simpler and more convenient preparation system is urgently needed. Meanwhile, under complex field conditions, the advantageous mechanism of the nanosize effect and sustained release have no quantitative and detailed study.

(4,5,8)-Connected Cationic Coordination Polymer Material as Explosive Chemosensor Based on the in Situ Generated AIE Tetrazolyl-Tetraphenylethylene Derivative.

A multidentate tetrazole molecule based on a TPE core, tetrakis[4-(1-tetrazol-5-yl)phenyl]ethylene (Httpe) with combined advantages of two functional groups, was synthesized by cycloaddition reaction of the corresponding organic benzonitrile derivative and azide salt. Coordination self-assembly of the in situ formed aggregation-induced emission polytetrazole luminogen with cadmium(II) ion produces an unprecedented tetrazolyl-TPE-based microporous cationic metal-organic framework (MOF) with an unusual (4,5,8T14)-connected net of {[Cd(Httpe)Cl]·(N)}, in which the Httpe serves as the first undeprotonated tetrazole ligand of octa-coordinating bridging mode. We investigate, for the first time, the utilization of the luminescent MOF containing a TPE core decorated with tetrazolyl terminals for explosive detection based on the change in fluorescence intensity, which shows high selectivity and efficiency in fluorescence quenching toward TNP detection in water solution.

The whole-genome and expression profile analysis of and in showed that and Dg may cooperate in the immunity against rust.

Orchardgrass () is one of the top four perennial forages worldwide and, despite its large economic advantages, often threatened by various environmental stresses. WRKY transcription factors (TFs) can regulate a variety of plant processes, widely participate in plant responses to biotic and abiotic stresses, and are one of the largest gene families in plants. WRKYs can usually bind W-box elements specifically.

CIRP promotes the progression of non-small cell lung cancer through activation of Wnt/β-catenin signaling via CTNNB1.

Background: Cold-inducible RNA binding protein (CIRP) is a newly discovered proto-oncogene. In this study, we investigated the role of CIRP in the progression of non-small cell lung cancer (NSCLC) using patient tissue samples, cultured cell lines and animal lung cancer models.

Methods: Tissue arrays, IHC and HE staining, immunoblotting, and qRT-PCR were used to detect the indicated gene expression; plasmid and siRNA transfections as well as viral infection were used to manipulate gene expression; cell proliferation assay, cell cycle analysis, cell migration and invasion analysis, soft agar colony formation assay, tail intravenous injection and subcutaneous inoculation of animal models were performed to study the role of CIRP in NSCLC cells; Gene expression microarray was used to select the underlying pathways; and RNA immunoprecipitation assay, biotin pull-down assay, immunopurification assay, mRNA decay analyses and luciferase reporter assay were performed to elucidate the mechanisms.

Herbivore-induced volatiles influence moth preference by increasing the β-Ocimene emission of neighbouring tea plants.

Herbivore-induced plant volatiles prime neighbouring plants to respond more strongly to subsequent attacks. However, the key volatiles that trigger this state and their priming mechanisms remain largely unknown. The tea geometrid Ectropis obliqua is one of the most devastating leaf-feeding pests of tea plants.

Self-Assembled Degradable Nanogels Provide Foliar Affinity and Pinning for Pesticide Delivery by Flexibility and Adhesiveness Adjustment.

At present, it is highly important to develop a simple and compatible nano delivery system for pesticides for foliar application, which can improve their insecticidal efficacy and resistance to adverse climates while reducing the environmental risks. Polyethylene glycol and 4,4-methylenediphenyl diisocyanate are used as hydrophilic soft and hydrophobic hard segments, respectively, for polymer self-assembly and polyurethane gelation in a nanoreactor. The nanocarrier synthesis and the pesticide loading are realized by a one-step integration procedure and suited well for hydrophobic active compounds.

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Oncogenic activation of KRAS and its surrogates is essential for tumour cell proliferation and survival, as well as for the development of protumourigenic microenvironments. Here, we show that the deubiquitinase USP12 is commonly downregulated in the Kras-driven mouse lung tumour and human non-small cell lung cancer owing to the activation of AKT-mTOR signalling. Downregulation of USP12 promotes lung tumour growth and fosters an immunosuppressive microenvironment with increased macrophage recruitment, hypervascularization, and reduced T cell activation.

Molecular Characterization and Elucidation of Pathways to Identify Novel Therapeutic Targets in Pulmonary Arterial Hypertension.

Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disease. However, there are limited studies reflecting the available biomarkers from separate gene expression profiles in PAH. This study explored two microarray datasets by an integrative analysis to estimate the molecular signatures in PAH.

Molecular cloning, sequence, and expression patterns of DNA damage induced transcript 3 (DDIT3) gene in female yaks ().

Endoplasmic reticulum stress (ERS) plays an important role in regulating the reproductive process of female mammals, mainly involved in follicular atresia and corpus luteum regression. DNA damage induced transcript 3 (DDIT3) is a marker gene of ERS. The objectives of the present study were to clone and analyze the sequence and tissue expression characteristics of DDIT3 gene in female yaks.

Deubiquitination of the repressor E2F6 by USP22 facilitates AKT activation and tumor growth in hepatocellular carcinoma.

Dysregulated ubiquitination of tumor-related proteins plays a critical role in tumor development and progression. The deubiquitinase USP22 is aberrantly expressed in certain types of cancer and contributes to aggressive tumor progression. However, the precise mechanism underlying the pro-tumorigenic function of USP22 in hepatocellular carcinoma (HCC) remains unclear.

A generalizable strategy for constructing ultralight three-dimensional hierarchical network heterostructure as high-efficient microwave absorber.

Using previous models and theories to construct and develop high-efficient microwave absorbers (MAs) should be a strategic and effective ways to optimize the electromagnetic wave attenuation. Herein, the ultralow density and flexible graphene oxide foam (GOF) and reduced graphene oxide foam (RGOF)/MoS nanosheets were designed and fabricated by the method of chemical vapor deposition and hydrothermal reaction. The obtained GOF and RGOF/MoS samples exhibited very excellent microwave absorption properties while their densities were merely 0.

The deubiquitinase USP16 functions as an oncogenic factor in K-RAS-driven lung tumorigenesis.

K-RAS mutation and molecular alterations of its surrogates function essentially in lung tumorigenesis and malignant progression. However, it remains elusive how tumor-promoting and deleterious events downstream of K-RAS signaling are coordinated in lung tumorigenesis. Here, we show that USP16, a deubiquitinase involved in various biological processes, functions as a promoter for the development of K-RAS-driven lung tumor.

Tumor targeting nanoparticle E7-HSP110-RGD elicits potent anti-tumor immune response in a CD8-dependent manner in cervical cancer-bearing mouse model.

Our previous research verified that HSP (heat shock protein) 110 could enhance the anti-tumor effect of HPV16 E7 epitope. In this study, to optimize the immunotherapy of this vaccine type, we developed and evaluated the anti-tumor immunity of a nanoparticle vaccine format assembling with E7-HSP110 fusion expression plasmid and RGD-GGG-K polypeptide. The nanoparticle vaccine was self-assembled from positively charged RGD-GGG-K polypeptide and negatively charged fusion expression plasmid pIRES2-3× E7-HSP110-EGFP.

Interaction between testosterone and obesity on hypertension: A population-based cross-sectional study.

Background And Aims: We aimed to evaluate the effects of serum testosterone, obesity and their interaction on blood pressure (BP) parameters and hypertension among Chinese rural adults.

Methods: A total of 6199 adults were recruited from the Henan Rural Cohort Study. Serum testosterone was measured by liquid chromatography-tandem mass spectrometry.

Corrigendum: Reprogramming of Cell Fate During Root Regeneration by Transcriptional and Epigenetic Networks.

[This corrects the article DOI: 10.3389/fpls.2020.

Evaluation of a Novel Three-Dimensional Emergency Cricothyrotomy Task Trainer.

Background Emergency cricothyrotomy is a critical, yet infrequently performed and time-sensitive procedure that requires practice in order to be reliably completed in emergent airway situations. Many physicians never have the opportunity to practice this rarely performed but highly impactful procedure during their training due to a lack of an affordable, high-fidelity training model. In this study, the educational impact and realism of a new synthetic cricothyrotomy training model (high-fidelity emergency cricothyrotomy, HiFEC) were compared with those of a standard porcine explant model.

Commonly and Specifically Activated Defense Responses in Maize Disease Lesion Mimic Mutants Revealed by Integrated Transcriptomics and Metabolomics Analysis.

Disease lesion mimic (/) mutants display disease-like spontaneous lesions in the absence of pathogen infection, implying the constitutive activation of defense responses. However, the genetic and biochemical bases underlying the activated defense responses in those mutants remain largely unknown. Here, we performed integrated transcriptomics and metabolomics analysis on three typical maize mutants , , and with large, medium, and small lesion size, respectively, thereby dissecting the activated defense responses at the transcriptional and metabolomic level.

Chromosome-level genome assembly of the Chinese longsnout catfish .

The Chinese longsnout catfish ( Günther) is one of the most economically important freshwater fish in China. As wild populations have declined sharply in recent years, it is also a valuable model for research on sexual dimorphism, comparative biology, and conservation. However, the current lack of high-quality chromosome-level genome information for the species hinders the advancement of comparative genomic analysis and evolutionary studies.

Lycopene Attenuates Hypoxia-Induced Testicular Injury by Inhibiting PROK2 Expression and Activating PI3K/AKT/mTOR Pathway in a Varicocele Adult Rat.

Purpose: The aim of this study was to evaluate the effect of lycopene on hypoxia-induced testicular injury in rat model and explore the underlying mechanism.

Methods: Six-week-old male Wistar rats ( = 36) were randomly divided into three groups ( = 12/group): a normal group (NG, sham control), a varicocele group (VG), and a varicocele treated by lycopene group (VLG). Bilateral renal veins constriction was performed on rats in VG and VLG.

STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins.

Many proteins produced in CHO cells need evaluation for their clinical and commercial potential. Traditional methods based on stable clone generation are slow and unsuitable for screening larger numbers of proteins, while transient expression technologies are fast but unpredictable regarding product quality and lacking an optional path to subcloning. The STEP® vector technology introduced here combines the best properties of both methods.