Octarepeat peptides of prion are essential for multidrug resistance in gastric cancer cells.

Objective: In previous studies cellular prion protein (PrPc) is confirmed to be involved in multidrug resistance (MDR) of gastric cancer. Although octarepeat peptides are important functional domains of PrPc and are closely related to the transport of Cu2+/Zn2+ and antioxidative function, the significance in MDR remains unknown. We aimed to investigate the role of octarepeat peptides in gastric cancer MDR.

Dynamic changes and surveillance function of prion protein expression in gastric cancer drug resistance.

Aim: To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.

Methods: A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established, and the expression of PrPc, Bcl-2 and Bax was detected in these cells. Apoptosis was determined using Annexin V staining.

[Effect of a hypothetical gene Af116609 on multi-drug resistance of gastric cancer cells].

Objective: To investigate the effect of gene Af116609 on gastric cancer multi-drug resistance (MDR) by introducing it into gastric cancer multi-drug resistant (MDR) cell line SGC7901/VCR.

Methods: Gene Af116609 was cloned from SGC7901/VCR by RT-PCR and its differential expression between gastric cancer MDR cells and its parental cells was displayed by Northern blot. The gene was introduced to gastric cancer cells by transfection of recombinant eukaryotic expression vector by electroporation.

[The overexpression and significance of MKP-1 in oxygen-deprived gastric cancer cell line SGC7901].

Objective: To investigate the expression status of Mitogen-activated Protein Kinase Phosphatase-1 in oxygen-deprived gastric cancer cell line SGC7901 and its role in HIF-1 regulation.

Methods: The expression of MKP-1 in gastric cell line SGC7901 was detected with Western blot and semiquantity RT-PCR; Eukaryotic sense expression vector was constructed based on DNA recombination technology. Transfections of SGC7901 were performed using liposome; The luciferase activity was determined using Dual Luciferase Reporter System and the levels of VEGF in SGC7901cells under normoxia and hypoxia were measured by ELISA.

[The expression and possible function of RhoA in human gastric cancer cell lines].

Objective: To study the expression and possible function of RhoA in human gastric cancer cell lines.

Methods: The expression of RhoA in human gastrointestinal cancer cell lines was detected by Western blot. Antisense plasmid of RhoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine.

[Clinical application of ventriculoperitoneal shunt assisted by laparoscope].

Objective: To compare the clinical effectiveness of ventriculoperitoneal shunt (VPS) assisted by laparoscope versus the clinical effectiveness of VPS via traditional laparotomy in treating hydrocephalus.

Methods: The clinical data on 26 cases of VPS with laparoscope assistance and on 234 cases of VPS via traditional laparotomy were summerized and analyzed retrospectively. The operation efficacy and the complication rate were compared between the two groups.

[Protective effect of selective cyclooxygenase-2 inhibitor on alcohol-induced liver injury in rats].

Objectives: To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor on alcohol-induced liver injury in rats.

Methods: 58 male Wistar rats were randomly divided into three groups: control group treated with dextrose and corn oil, model group with ethanol and corn oil, treatment group with corn oil and ethanol plus a selective COX-2 inhibitor celecoxib. All treatments were injected into stomach through intragastric tubes.

[Rac subfamily expression and activity in gastrointestinal cancer cell lines].

Objective: To investigate the significance of Rac subfamily members in the gastrointestinal carcinogenesis and progression.

Methods: The mRNA expression of Rac1, Rac2 and Rac3 in 12 kinds of gastrointestinal cancer cell lines was examined by semi-quantitative RT-PCR. The activities of Rac1 protein in 5 kinds of gastric cancer cell lines were tested by pull-down assay.

[The overexpression of prion protein in drug resistant gastric cancer cell line SGC7901/ADR and its significance].

Objective: To investigate the overexpression of prion protein (PrP) in drug-resistant gastric cancer cell line SGC7901/ADR and its role in multidrug resistance in gastric cancer.

Methods: (1) The expression of PrP in SGC7901/ADR, SGC790/VCR and their parental cell line SGC7901 was detected with Northern and Western blot at the mRNA and protein level. (2) Eukaryotic sense and antisense expression vector were constructed based on DNA recombination technology and (3) introduced into SGC7901 and SGC7901/ADR cell lines through electroporation.

[Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance].

Objective: To investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.

Methods: Total RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.

[Effects of different temperature and time on the period of validity and quality in blood preservation].

Aim: To examine the effects of different temperature protection on measures on preservation damages in liquid blood and explore the corresponding.

Methods: Take equal half blood samples from 10 healthy blood donors and divided each sample into two groups, put the fresh blood into CP2D-A solution at 0 degrees C and 4 degrees C, respectively and take the samples 21 days and 42 days, later and then measured the contents of membrane phospholipids with shafig-UR-rehman method, CaM with purification PED test, LPO with spectrophotometry.

Results: At the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4 degrees C.

[Effects of different temperature and time on the quality of blood preservation in liquid blood].

Aim: To explore the effects of different temperature and time on preservation damages in liquid blood.

Methods: Take blood sample from 10 healthy blood donors, put the fresh blood into CP2D-A liquid at 0 degrees C and 4 degrees C, and take the samples after 1 week, 2 weeks and 3 weeks, and then measured the contents of GSH-Px, TSH, LPO, the contracting protein of RBC, and membrane fluidity.

Results: At the same temperature, when the preservation time is prolonged, peroxidation is increased, the preservation damages are also augmented; the damages are declined when the temperature is lower during the same period, the aging of blood was more evident at 4 degrees C.

Effect of apolipoprotein E gene Hha I restricting fragment length polymorphism on serum lipids in cholecystolithiasis.

AIM:To investigate the role of apolipoprotein E (apoE) polymorphism in the lithogenesis of gallstone and the hereditary pathogenesis of the disease.METHODS: Polymerase chain reaction (PCR) was used to study apoE phenotypes and allele frequencies in patients with gallstones and control, and the fasting serum lipids of subjects were also measured by enzymatic methods.RESULTS:The levels of triglyceride (TG) and very low density lipoprotein cholesterol (VLDL-C) were much higher in E(2/3) patients than that in E(2/3) control.