Non-invasive prenatal detection of dominant single-gene disorders in fetal structural abnormalities: a clinical feasibility study.

Objective: This study evaluated the accuracy of non-invasive prenatal testing (NIPT-SGDs) for dominant monogenic genetic diseases associated with fetal structural abnormalities and to assess the feasibility of clinical application.

Methods: Pregnant women requiring prenatal diagnosis due to fetal structural abnormalities were enrolled. Maternal peripheral blood was analyzed for cell-free DNA (cfDNA) using coordinative allele-aware target enrichment sequencing (COATE-seq).

Strategies for the development of stimuli-responsive small molecule prodrugs for cancer treatment.

Approved anticancer drugs typically face challenges due to their narrow therapeutic window, primarily because of high systemic toxicity and limited selectivity for tumors. Prodrugs are initially inactive drug molecules designed to undergo specific chemical modifications. These modifications render the drugs inactive until they encounter specific conditions or biomarkers , at which point they are converted into active drug molecules.

Nursing Workloads and Lower Urinary Tract Symptoms Among Chinese Female Nurses: The Mediating Role of Delayed Voiding Behaviors.

Purpose: Lower urinary tract symptoms (LUTS) are a challenge to the healthy nursing workforce. Nurses confront various objective and subjective nursing workloads which contribute to their LUTS, but less is known about how nursing workloads influence their LUTS. This study is designed to test hypotheses that delayed voiding behaviors mediate the relationships between nursing workloads and LUTS in female nurses.

Difference analysis of cutaneous sporotrichosis between different regions in China: a secondary analysis based on published studies on sporotrichosis in China.

Background: Cutaneous sporotrichosis is prevalent worldwide and a common subcutaneous fungal disease in northeast China. The incidence of cutaneous sporotrichosis in southern China cannot be ignored. Previous studies have revealed differences between different regions in China regarding populations susceptible to infection, cause of infection, and pathological mechanisms of sporotrichosis.

Identification of a rare missense mutation in GJB1 and prenatal diagnosis in a Chinese family with CMT: A case report.

Rationale: Charcot-Marie-Tooth disease (CMT) is a highly heterogeneous genetic disorder. To date, more than 90 genes have been implicated in the pathogenesis of CMT. Here, we report the identification of a rare causative mutation in a Chinese family with CMT and a pregnant patient underwent prenatal diagnosis.

Behavior of and in Raw Yellowfin Tuna during Cold Storage.

Behavior of and in raw yellowfin tuna during refrigeration and frozen storage were studied. Growth of was inhibited in tuna during refrigerated storage, while was able to multiply significantly during refrigerated storage. Populations of in tuna were reduced by 1 to 2 log after 12 days of storage at 5-7 °C, regardless levels of contamination.

Analysis of treatment effect of urinary kallidinogenase combined with edaravone on massive cerebral infarction.

The aim of the study was to investigate the clinical effect of urinary kallidinogenase combined with edaravone in the treatment of massive cerebral infarction. A total of 58 patients with massive cerebral infarction were admitted to hospital between January 2013 and January 2014. There were 34 male and 24 female patients.

Fuzhisan, a chinese herbal medicine, suppresses beta-secretase gene transcription via upregulation of SIRT1 expression in N2a-APP695 cells.

The accumulation of β-amyloid (Aβ) peptide plaques is the major pathogenic event in Alzheimer's disease (AD). Because β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) cleaves APP at the first amino acid of the Aβ domain and is the rate-limiting enzyme for Aβ peptide generation, the level of this aspartic protease is a focus of AD research. Fuzhisan (FZS), a Chinese herbal complex prescription that has been used for the treatment of AD for over 20 years, is known to enhance metabolic activity and cognitive ability in aged rats and AD patients.

Reduction of α-dystroglycan expression is correlated with poor prognosis in glioma.

Dystroglycan (DG), a multifunctional protein dimer of non-covalently linked α and β subunits, is best known as an adhesion and transduction molecule linking the cytoskeleton and intracellular signaling pathways to extracellular matrix proteins. Loss of DG binding, possibly by degradation or disturbed glycosylation, has been reported in a variety of cancers. DG is abundant at astroglial endfeet forming the blood-brain barrier (BBB) and glia limitans; so, we examined if loss of expression is associated with glioma.

Zinc supplementation in acute diarrhea.

Objective: To determine the effect of zinc supplementation on the duration of acute diarrhea in children.

Methods: A search was conducted in PubMed, the Cochrane Library database, China Biology Medicine (CBM), CNKI, VIP, and Wanfang Data to find studies on zinc supplementation for children with acute diarrhea from the date of establishment of the respective databases to June 2013. Clinical trials were included, and reviews or other types of studies were excluded.

High-level expression of heme-dependent catalase gene katA from Lactobacillus Sakei protects Lactobacillus rhamnosus from oxidative stress.

Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product fermentation, but they are deficient in catalase. In this study, the effect of heterologous expression of L.

Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA.

Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis (TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine industry. Previously, we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA polymerase (RdRP) gene of TGEV with more than 95% of virus inhibition in vitro. In this study, inhibition of the TGEV replication by pEGFP-U6/P1 and pEGFP-U6/P2 was tested in mini-pigs.

Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method.

Genotype 4 hepatitis E virus (HEV) was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate (SH-SW-zs1) recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR and RACE (Rapid Amplification of cDNA Ends) methods. The full genome of the SH-SW-zs1 isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype 4.