Triazine-cored covalent organic framework for ultrasensitive detection of polybrominated diphenyl ethers from real samples: Experimental and DFT study.

Food and environmental safety issues attributable to the polybrominated diphenyl ethers (PBDEs) are gaining increasing attention, and these urge us to establish a high-performance sample-handling technique. In this study, an outstanding adsorption performance with short adsorption time (10 min) was achieved for PBDEs using a novel synthesized dispersive solid-phase extraction adsorbent, a reticulated covalent organic framework with N/O functional groups (i.e.

Dangguishaoyao-San attenuates LPS-induced neuroinflammation via the TLRs/NF-κB signaling pathway.

Introduction: Dangguishaoyao-San (DSS) is composed of six traditional Chinese medicines, including Angelica sinensis, Paeoniae radix, Rhizoma Ligusticum, Poria cocos, Rhizoma Atractylodis Macrocephalae, and Rhizoma Alismatis. DSS has been reported to be effective in alleviating the symptoms of Alzheimer's disease (AD). The aim of this study was to investigate the mechanism of action of DSS in vitro using lipopolysaccharide (LPS)-stimulated BV-2 microglia cells.

[Effect of Herba Scutellariae Barbatae flavonoids in delaying aging of Caenorhabditis elegans and human umbilical vein endothelial cells in vitro].

Objective: To explore the effects of Herba Scutellariae Barbatae flavonoids (HF) in delaying aging of Caenorhabditis elegans and human umbilical vein endothelial cells (HUVECs) in vitro.

Methods: The effects of 30 or 50 mg/L of HF on nematode life span, reproductive capacity, oxidative stress, and antioxidant enzyme activity of C. elegans were assessed, and the effects of HF on the expressions of the genes encoding antioxidant enzymes and the aging-related genes were analyzed using real-time RT-PCR in both C.

[Comparison of Chemical Components of Essential Oil from Ocimum basilicum var. pilosum Extracted by Supercritical CO2 Fluid and Steam Distillation].

Objective: To compare the chemical components of essential oil prepared by steam distillation extraction (SD) and supercritical CO2 fluid extraction (SFE-CO2) from Ocimum basilicum var. pilosum whole plant.

Methods: The essential oil of Ocimum basilicum var.

Efficacy and Safety of Duloxetine on Osteoarthritis Knee Pain: A Meta-Analysis of Randomized Controlled Trials.

Objectives: The aim of this meta-analysis was to evaluate the efficacy and safety of duloxetine for management of osteoarthritis knee (OAK) pain.

Methods: A systematic literature search of articles for management of OAK using duloxetine were performed in PubMed, EBSCO, EMBASE, ScienceDirect, MEDLINE, ClinicalTrials.gov, Google Scholar, and Cochrane Central Register of Controlled Trials from the available date of inception until the latest issue (October 2013).

[Comparison of the chemical components of essential oil extracted by supercritical CO2 fluid and steam distillation from Pteris multifida].

Objective: To compare the chemical components of essential oil extracted by supercritical CO2 fluid extraction (SFE-CO2) and steam distillation extraction (SD) from Pteris multifida.

Methods: The essential oil of Pteris multifida was extracted by SFE-CO2 and SD. The chemical components of essential oil were separated and analyzed by GC-MS.

[Content analysis of total polyphenol in the leaves of Jatrophy curcas. L].

Objective: To establish a method for analyzing the content of total polyphenols in leaves of Jatropha curcas. L.

Methods: Gallic acid was used as reference substance, the content of total polyphenols was analyzed Folin-Ciocalteu chromatometry.

[Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction].

Objective: To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).

Methods: The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.

[Sensitivity of Jatropha curcas seeds to (60)Co-gamma radiation and their medial lethal doses in radiation breeding].

Objective: To study the sensitivity of Jatropha curcas seeds from three different locations to (60)Co-gamma radiation and to determine the medial lethal doses (LD50) of (60)Co-gamma radiation for these seeds.

Methods: Six different radiation doses (0, 100, 150, 200, 250 and 300 Gy) were used. Based on the germination rate 50%, LD50 doses of (60)Co-gamma radiation for the seeds were calculated using linear regression equation.

[Oil contents and fatty acid composition in Jatropha curcas seeds collected from different regions].

Objective: To compare the oil contents and fatty acid composition among the samples of Jatropha curcas L. seeds collected from China (Guangdong, Hainan, and Guizhou Provinces) and India.

Methods: Soxhlet extraction method and gas chromatography-mass spectrometry (GC-MS) were employed to determine the oil contents of Jatropha seeds and the fatty acid composition of Jatropha oil.

[The protective effect of Astragalus membranaceus extraction on cryopreserved primary-cultured human fetal hepatocytes].

Objective: To observe the influence of Astragalus membranaceus Extraction (AE) on the primary-cultured human fetal hepatocytes stored in liquid nitrogen and explore a new method for the cryopreservation of human hepatocytes with improved function.

Methods: Human fetal hepatocytes were harvested by two-step collagenase perfusion, and then stored in a liquid nitrogen for one month with five different cryoprotectants (I: 10% DMSO, II: 5% DMSO + 2 mg/L AE, III: 5% DMSO + 20 mg/L AE, IV: 5% DMSO + 60 mg/L AE, V: 5% DMSO + 100 mg/L AE). One month later, the cells were thawed rapidly and the viability, morphology and basic function of them were tested.

[Tumor-specific T cell recptor gene transfection promotes memory T cell differentiation in vitro].

Objective: To investigate effect of tumor-specific T cell receptor gene transfection on memory T cell differentiation in vitro.

Methods: TCRVbeta7.1 gene was transferred into peripheral blood mononuclear cells (PBMCs) obtained from healthy adults, and the expression of Vbeta7.

[Efficient and rapid non-test tube cloning of Jatropha curcas].

Objective: To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas.

Methods: Using the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied.

Results And Conclusion: When treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation.

[Antitumor effect of GPI-CD80 fusion protein in nude mice].

Objective: To study the antitumor effect GPI-CD80 fusion protein and its mechanisms.

Methods: A tumor vaccine was prepared by culturing HepG2 cells in the presence of purified GPI-CD80 followed by inactivation with mitomycin, with mitomycin-inactivated HepG2 cells as the control group. The two preparations were co-cultured with nude mouse splenic lymphocytes, and the changes of lymphocyte proliferation and the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) were detected by MTT assay.

[Effect of Scutellaria barbata extract against human hepatocellular Hep-G2 cell proliferation and its mechanism].

Objective: To observe the effects of Scutellaria barbata extract (ESB) on human hepatoma cell line Hep-G2 proliferation in vitro and explore the mechanism.

Methods: The inhibitory effect of ESB on Hep-G2 proliferation was estimated by MTT assay, and the morphological changes of the cells were observed under optical and electron microscopes. Distribution of cell cycle, cell apoptosis and the protein expressions of apoptosis-associated genes as bcl-2, bax and fas were analyzed using flow cytometry.

[Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells].

Objective: To investigate the inhibition effect of Scutellariae barbata extracts on the proliferation of human hepatocellular carcinoma cell line QGY-7701.

Methods: The inhibition activity of the extracts against cell line QGY-7701 was estimated by MTT assay. Morphologic changes of the cells were observed under light microscope and electronic microscope.

[Effect of koumine on proliferation of murine CD4+ T cells purified by magnetic-activated cell sorting in vitro].

Objective: To assess the separation efficiency of magnetic-activated cell sorting in the purification of CD4+ T cells from murine spleen, and observe the effects of koumine on the proliferation of the separated cells.

Methods: CD4+ T cells were isolated from murine spleen by magnetic-activated cell sorting (MiniMACS). Fluorescence-activated cell sortering was employed to determine the purity of CD4+ T cells before and after the separation procedure followed by evaluation of the cell viability using trypan blue staining.

[Determination of scopoletin and umbelliferone contents in Saussurea medusa Maxim by high-performance liquid chromatography].

The contents of scopoletin and umbelliferone in Saussurea medusa Maxim were determined by high-performance liquid chromatography (HPLC) using Merck Lichrospher100 RP-18e column (250 mm x 4.0 mm, 5 microm) with the mobile phase of methanol-tetrahydrofuran-water solution and the absorbance of the compounds observed at 346 nm. HPLC yielded an average recovery and RSD of 100.

[Infrared spectrum and nuclear magnetic resonance analysis of the alkaloid extract of Gelsemium produced in east Guangdong province].

Objective: To identify the structure of the alkaloid extract of Gelsemium from east Guangdong province of China.

Methods: Fourier transform infrared (FTIR) absorption spectrometry, hydrogen and carbon spectra of nuclear magnetic resonance (NMR) and distortionless enhancement by polarization transfer (DEPT) analysis were used for the structural identification of the alkaloid exstract of Gelsemium.

Results: The frequency, intensity and shape of the extract's characteristic peaks in infrared absorption spectra (4,000.

[Extraction and separation of koumine from Gelsemium alkaloids].

Objective: To establish a method for isolating and identifying koumine.

Methods: Koumine was first extracted from Gelsemium alkaloids by using chloroform, which was further separated and purified by column chromatograph, and identified by thin-layer chromatography.

Results And Conclusion: Koumine crystals were isolated and purified from Gelsemium alkaloids effectively.

[Comparative study of uniform design and orthogonal design in the extraction of Gelsemium alkaloids].

Objective: To develop a simple and efficient method for extracting Gelsemium alkaloids.

Methods: Uniform design and orthogonal design were respectively utilized to optimize the 3 factors in the extraction process, namely the type of solvent used, the solid-to-liquid ratio in the extraction system and the reflux time.

Results: Both of the design methods adopted trichloromethane as the solvent, with the solid-to-liquid ratio of 1:7.

[Association between gene mutation of cytochrome P450 1A1 in exon 7 A4889G locus and susceptibility to endometriosis].

Objective: To assess the possible association between gene mutation of cytochrome P450 1A1(CYP1A1) in exon 7 A4889G locus and the susceptibility to endometriosis (EM).

Methods: Allele specific-polymerase chain reaction method was used to analyze gene mutation in exon 7 A4889G locus of CYP1A1 in 76 patients with endometriosis and 80 healthy controls.

Results: The frequency of allele G on A4889G locus of CYP1A1 gene showed a significant difference between the study cohort and the control group (Chi2=7.

[Association between glutathione S-transferase M1 gene deletion and genetic susceptibility to endometriosis].

Objective: To evaluate the possible association of the glutathione S-transferase M1 (GSTM1) gene polymorphism with the susceptibility to endometriosis in women of Han nationality in Guangdong Province.

Methods: Polymerase chain reaction was used to identify the GSTM1 genotypes in 76 patients with endometriosis and 80 controls (surgical patients for gynecological problems other than endometriosis).

Results: The frequencies of the GSTM1 null genotypes in patients with endometriosis and controls were 65.

[Observations on the efficacy of Botai ointment in treating dermatosis common among armed forces receiving at-sea training].

Objective: To observe the efficacy of Botai ointment in the treatment of common dermatosis.

Methods: Botai ointment was the only drug applied for the treatment. The ointment was smeared on the affected area twice daily for 4 weeks.