Identification and characterization of a Streptococcus suis immunogenic ornithine carbamoytransferase involved in bacterial adherence.

Background: Streptococcus suis (SS) is a major swine pathogen and a serious zoonotic pathogen causing septicemia and meningitis in piglets and humans. Using an immunoproteomic approach, we previously brought evidence that ornithine carbamoytransferase (OCT) may represent a vaccine candidate to protect against S. suis biofilm-related and acute infections.

The preventive effects of hyperoside on lung cancer in vitro by inducing apoptosis and inhibiting proliferation through Caspase-3 and P53 signaling pathway.

Though advanced surgical operation and chemotherapy have been under taken, lung cancer remains one of the most aggressive and fatal human malignancies with a low survival rate. Thus, novel therapeutic strategies for prevention and remedy are urgently needed in lung cancer. Hyperoside, known as quercetin-3-O-β-d-galactopyranoside, is a natural flavonol glycoside discovered in plants of genera Hypericum, displaying anti-oxidant, anticancer, and anti-inflammatory properties.

[Association between interleukin-1B polymorphisms and chronic obstructive pulmonary disease: a meta-analysis].

Objective: To explore the association between the -511C/T, -31T/C and +3954C/T polymorphisms of interleukin-1B (IL-1B) gene and chronic obstructive pulmonary disease (COPD) using the method of meta-analysis.

Methods: Comprehensive searches were performed of PubMed, Embase, Ovid, Wanfang and Chinese journal full-text database to retrieve the related case-control studies. The pooled odds ratios were performed respectively for allele comparison, additive genetic model, dominant genetic model and recessive genetic model and subgroup analysis was also performed by ethnicity.

Chronic obstructive pulmonary disease assessment test score correlated with dyspnea score in a large sample of Chinese patients.

Background: The chronic obstructive pulmonary disease assessment test (CAT) is an easy to use health-related quality of life questionnaire, the modified Medical Research Council (mMRC) dyspnea scale is a classic dyspnea scale which is widely used, while the correlation between them is still not clear. This study investigated the use of the Chinese translation of CAT in chronic obstructive pulmonary disease patients and its correlation with the mMRC dyspnea scale.

Methods: The multicenter cross-sectional study was conducted in 329 hospitals throughout China from March 1 to April 30, 2010.

Expression, refolding, and characterization of human soluble BAFF synthesized in Escherichia coli.

The B lymphocyte stimulator (BAFF) is a novel member of the tumor necrosis factor (TNF) ligand family which is important in B lymphocyte maturation and survival. Here, a recombinant form of the extracellular domain of the BAFF (hsBAFF) was expressed in Escherichia coli BL21(DE3) under the control of a T7 promoter. The resulting insoluble bodies were separated from cellular debris by centrifugation and solubilized with 8 M urea.