The accuracy of spot sign in predicting hematoma expansion after intracerebral hemorrhage: a systematic review and meta-analysis.

Purpose: The role of spot sign on computed tomography angiography (CTA) for predicting hematoma expansion (HE) after primary intracerebral hemorrhage (ICH) has been the focus of many studies. Our study sought to evaluate the predictive accuracy of spot signs for HE in a meta-analytic approach.

Materials And Methods: The database of Pubmed, Embase, and the Cochrane Library were searched for eligible studies.

[Efficacy of tigecycline in treating severe infections of patients with hematological diseases].

Objective: To evaluate the efficacy and safety of tigecycline in treating secondary infections of patients with hematological diseases.

Methods: A total of 85 cases of hematological patients with secondary infections were classified into empirical and targeted therapy groups. Empirical therapy group was composed of patients receiving tigecycline as an alternative due to ineffective anti-infection treatment for 3-5 days in the absence of microbiological evidence while those taking tigecycline based on microbiological evidence belonged to targeted therapy group.

Hydraulic optimization of membrane bioreactor via baffle modification using computational fluid dynamics.

Baffles are a key component of an airlift membrane bioreactor (MBR), which could enhance membrane surface shear for fouling control. In order to obtain an optimal hydraulic condition of the reactor, the effects of baffle location and size were systematically explored in this study. Computational fluid dynamics (CFD) was used to investigate the hydrodynamics in a bench-scale airlift flat sheet MBR with various baffle locations and sizes.

[Efficacy of wound dressing with microspheres containing levofloxacin on burns treatment].

This research was aimed to find the skin irritation and burns treatment effect of wound dressing with mi- crospheres containing levofloxacin. We used reference GB/T16886. 10-2005 to evaluate the dressing skin irritation.

Iodine-125 brachytherapy improved overall survival of patients with inoperable stage III/IV non-small cell lung cancer versus the conventional radiotherapy.

This study compared the efficacy of iodine-125 seed brachytherapy versus the conventional radiotherapy in patients with non-resectable stage III/IV non-small cell lung cancer. A total of 71 patients with inoperable advanced stages of lung cancer with tumor size ranging 5-10 cm were randomly assigned into two groups: Group A received the regional iodine-125 implantation (n = 35), and Group B received the conventional radiotherapy (n = 36). The isodose curves were obtained by the treatment planning system for patients in Group A.

Comparison of waveguide properties and Raman spectroscopic visualization of C and O ion implantation on LaAlO(3) crystals.

LaAlO crystals were implanted by C ions and O ions at an energy of 6.0 MeV with a fluence of 1.5×10  ions/cm.

Possible oriented transition of multiple-emulsion globules with asymmetric internal structures in a microfluidic constriction.

When a globule with a complete symmetry (such as simple spherical droplets and concentric double emulsions) is transiting in a constriction tube, there is only one pattern of the transition. However, for a multiple-emulsion globule with asymmetric internal structures, there are many possible patterns with different pressure drops Δp due to various initial orientations of the inner droplets. In this paper, a boundary integral method developed recently is employed to investigate numerically the possible oriented transition of a globule with two unequal inner droplets in an axisymmetric microfluidic constriction.

HDL cholesterol and cancer risk among patients with type 2 diabetes.

Objective: To investigate the relationship between HDL cholesterol (HDL-C) and cancer risk among type 2 diabetic patients.

Research Design And Methods: We performed a retrospective cohort study of 14,169 men and 23,176 women with type 2 diabetes. Cox proportional hazards regression models were used to estimate the association of various levels of HDL cholesterol (HDL-C) with cancer risk.

[Memory B (CD5⁺ CD19⁺ CD27⁺) lymphocyte in patients with immune-related pancytopenia].

Objective: To detect memory B lymphocyte (Bm) in peripheral blood (PB) of immune-related pancytopenia (IRP).

Methods: 86 patients with IRP and 11 health volunteers were enrolled in this study. Bm (CD5⁺ CD19⁺ CD27⁺) and bone marrow mononucleated cell antibodies (BMMNC-Ab) were determined via fluorescence-activated cell sorting, and clinical outcomes of these patients were analyzed.

[Effect of non-ionic surfactants on butanol production with Clostridium acetobutylicum ATCC 824].

The low butanol concentration of acetone-butanol-ethanol fermentation causes uneconomical product recovery. In this work, the effect of small molecule non-ionic surfactants on butanol fermentation was evaluated, using laboratory stocks of Clostridium acetobutylicum ATCC 824. Non-ionic surfactants substantially increased butanol production when additive amount was higher than 1% (W/W).

Targeted high-throughput sequencing identifies pathogenic mutations in KCNQ4 in two large Chinese families with autosomal dominant hearing loss.

Autosomal dominant non-syndromic hearing loss (ADNSHL) is highly heterogeneous, among them, KCNQ4 is one of the most frequent disease-causing genes. More than twenty KCNQ4 mutations have been reported, but none of them were detected in Chinese mainland families. In this study, we identified a novel KCNQ4 mutation in a five generation Chinese family with 84 members and a known KCNQ4 mutation in a six generation Chinese family with 66 members.

Differential evolution with auto-enhanced population diversity.

In differential evolution (DE) studies, there are many parameter adaptation methods, aiming at tuning the mutation factor F and the crossover probability CR . However, these methods still cannot resolve the issues of population premature convergence and population stagnation. To address these issues, in this paper, we investigate the population adaptation regarding population diversity at the dimensional level and propose a mechanism named auto-enhanced population diversity (AEPD) to automatically enhance population diversity.

[Effect of CCL3 on osteoblast in myeloma bone disease].

Objective: To culture osteoblast in vitro and evaluate CCL3 receptor CCR1 expression in patients with multiple myeloma (MM).

Methods: Bone marrow osteoblasts from MM patients were cultured in vitro with dexamethasone, β-sodium glycerophosphate and vitamin C, which were identified by alkaline phosphatase staining, Von Kossa's staining. The CCL3 receptor expression was evaluated by flow cytometry.

[Telomere length of peripheral lymphocytes in patients with immuno-related pancytopenia].

Objective: To investigate the changes of relative telomere length (RTL) of peripheral blood (PB) CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T lymphocytes, CD19⁺B lymphocytes and bone marrow (BM) CD34⁺ cells and its association with disease severity in untreated patients with immuno-related pancytopenia (IRP).

Methods: The PB CD3⁺ , CD3⁺ CD4⁺ , CD3⁺ CD8⁺ T lymphocytes, CD19⁺ B lymphocytes, and BM CD34⁺ cells were purified by magnetic activated cell sorting (MACS), and RTL were measured with flow-fluorescence in situ hybridization (FLOW-FISH).

Results: The RTL of CD3⁺, CD3⁺CD4⁺ , and CD3⁺CD8⁺T lymphocytes in untreated IRP patients were (27.

[Abnormal WT1 gene expression in paroxysmal nocturnal hemoglobinuria].

Objective: To explore the pathogenesis of abnormal WT1 expression in paroxysmal nocturnal hemoglobinuria (PNH).

Methods: The expression of WT1 mRNA in CD59⁻ and CD59⁺ bone marrow mononuclear cells (BMMNC) were measured by semi-quantitative reverse transcription PCR. After WT1 gene silence by RNA interference (RNAi) technology, biological characteristics of BMMNC were investigated by flow cytometry.

[Quantity and function of T follicular helper cells in the bone marrow of patients with immune thrombocytopenia].

This study was purposed to detect the quantity and function of bone marrow (BM) T follicular helper (Tfh) cells of patients with immune thrombocytopenia, and to explore the role of Tfh cells in the pathogenesis of ITP. Twenty-one newly diagnosed ITP patients, twenty ITP patients in recovery stage and eighteen normal controls were enrolled in this study. The percentages of Tfh cells, Tfh-related molecules ICOS, CD40L, IL-21 in BM were detected by flow cytometry (FCM), and the mRNA expression of BCL-6 in BMMNC was determined by semi-quantitive RT-PCR.

[Plasma DNA methylation of shp1 in patients with diffuse large B cell lymphoma].

Objective: To explore the methylation status of shp1 gene in plasma DNA from patients with diffuse large B cell lymphoma (DLBCL) and discuss its possible application in molecular diagnosis and targeted therapy of the disease.

Methods: Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation status of shp1 gene in plasma and peripheral blood leukocytes (PBLs) of 35 DLBCL patients. The formaldehyde-fixed, paraffin-embedded (FFPE) tumor tissue samples were collected from 28 DLBCL patients, 6 patients of benign lymphoid hyperplasia and 13 healthy volunteers were selected as nonmalignant controls from January 2012 to December 2013.

[Changes of natural kill cell in peripheral blood of patients with myelodysplastic syndrome].

Objective: To analyze the percentage and functional changes of natural kill (NK) cell in peripheral blood of myelodysplastic syndrome (MDS) patients so as to evaluate the relationships between these changes and hematopoietic functions and explore the role of NK cell in the pathogenesis of MDS.

Methods: By flow cytometry, the percentage of NK cell (CD3⁻CD56⁺CD16⁺) in peripheral blood lymphocytes was detected in 20 MDS patients, 15 acute myelogenous leukemic (AML) and 15 normal controls from March 2013 to November 2013 at our hospital.NK cell activation receptors (NKG2D, NKp44), inhibitory receptors (CD158a, CD158b), perforin and granzyme-β of patients and normal controls were also detected.

[Expression and clinical significance of miR-21 in diffuse large B cell lymphoma].

This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma (DLBCL) paraffin-embedded tissues, and evaluate its potential relevance with clinical characteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymph node tissue specimens were examined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining.

Elevated TIM3+ hematopoietic stem cells in untreated myelodysplastic syndrome displayed aberrant differentiation, overproliferation and decreased apoptosis.

TIM3, as a negative regulator of anti-tumor immunity, is highly expressed on LSCs, but not on normal HSCs. TIM3 on HSCs in MDS patients has not been clarified. Here, both the percentage of TIM3 on HSCs and the MFI of TIM3+ HSCs were higher in untreated MDS than control and were closed to AML, and excessive TIM3+ HSCs was closely related to clinical parameters: WPSS score, karyotype analysis, morphologic blasts, the number of cytopenia involving hematopoietic lineages, anemia and granulocytopenia.

Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation.

A new method for imaging the tumor human vascular endothelial growth factor 165 (VEGF 165) is presented. A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165.

[The study of the pure tone audiometry characteristics and curative effect in sudden hearing loss patients with hypertension].

Objective: To investigate the pure tone audiometry characteristics and curative effect in sudden hearing loss patients with hypertension.

Method: One hundred and fifty-seven inpatients (168 ears) with hypertension suffered from sudden hearing loss were included in this study. We retrospectively analyzed the audiological index of these patients by comparing the pure tone audiometry (PTA) among patients in the aspects of gender, age, affected side, duration of hypertension, with or without inducement, concomitant symptoms and other combined diseases.

[Analysis of characteristics of tinnitus in patients with auditory neuropathy spectrum disorder].

Objective: To investigate the characteristics of tinnitus in patients with auditory neuropathy spec- trum disorder (ANSD).

Method: This study recruited 14 ANSD patients with tinnitus. All the ANSD patients un- derwent detailed history taking, audiological examinations and assessments of tinnitus.

Pd(II)-catalyzed mild C-H ortho arylation and intramolecular amination oriented by a phosphinamide group.

A novel protocol for the Pd-catalyzed ortho-arylation of aryl phosphinamide with boronic acid is reported. By using phosphinamide as a new directing group, the reaction proceeds efficiently under mild conditions at 408C. Mechanistic studies reveal that the reaction proceeds via a PdII to Pd0 cycle.

Abnormalities of quantities and functions of linker for activations of T cells in severe aplastic anemia.

Objective: Severe aplastic anemia (SAA) is a rare immune-regulated disease characterized by severe pancytopenia and bone marrow failure, caused by destruction of hematopoietic cells by the activated T lymphocytes. Linker for activation of T cells (LAT), a transmembrane adaptor protein, plays a key role in T-cell and mast cell functions. However, it remains unclear how LAT may change in patients with SAA.