Identification of Active Bronchioalveolar Stem Cells as the Cell of Origin in Lung Adenocarcinoma.

Unlabelled: While initiation is established as a critical step in tumorigenesis, the identity of the cell of origin for lung adenocarcinoma and the mechanism controlling susceptibility to initiation remain elusive. Here we show that lung tumor suppressor Gprc5a-knockout (KO) mice are susceptible to initiation of lung tumorigenesis. Bronchioalveolar stem cells (BASC) and alveolar type 2 (AT2) cells were aberrantly expanded in Gprc5a-KO mouse lungs compared with those in wild-type (WT) mice, suggesting that Gprc5a-KO might confer susceptibility to initiation by increasing the cell of origin in mouse lungs.

Study on temporal and spatial evolution law for dust pollution in double roadway ventilation system of short wall continuous mining face.

To explore the laws of variations in the evolution of dust pollution within a double tunnel ventilation system at a short wall continuous mining face, a numerical simulation of air flow movement was conducted in this study. Results showed that after the wind flowing in the supporting and heading tunnels reached the head, the wind sides returned at speeds of 6.5 and 10.

Multi-functional Potential of Five Lactic Acid Bacteria Strains Derived from Giant Panda (Ailuropoda melanoleuca).

The multi-functional properties of lactic acid bacteria (LAB) on host health have been a popular research topic. The aim of present study was to assess the multi-functional potential of five LAB strains isolated from giant panda. In this study, we analyzed five giant panda LAB strains (Weissella confuse WJ202003 (W3), WJ202009 (W9), WJ202021 (W21), BSP201703 (X); Lactiplantibacillus plantarum BSGP201683 (G83)) and found that they exhibited rapid growth as well as strong acid production capacity.

FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.

Pancreatic cancer is regarded as the most lethal solid tumor worldwide. Deregulated and constitutively activated NF-κB signaling is one of the major characteristics of pancreatic cancer. The total expression level and subcellular localization of RelA/p65 have been shown to determine the activation of canonical NF-κB signaling in pancreatic cancer.

Very Low Frequency Radial Modulation for Deep Penetration Contrast-Enhanced Ultrasound Imaging.

Contrast-enhanced ultrasound imaging allows vascular imaging in a variety of diseases. Radial modulation imaging is a contrast agent-specific imaging approach for improving microbubble detection at high imaging frequencies (≥7.5 MHz), with imaging depth limited to a few centimeters.

The Panda-Derived G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.

Intestinal diseases are one of the main causes of captive giant panda death. Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection. The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.

Seasonal monitoring of Cryptosporidium species and their genetic diversity in neonatal calves on two large-scale farms in Xinjiang, China.

To find out whether and how the prevalence and genetic diversity of Cryptosporidium in neonatal calves vary with the season, 380 fecal samples from neonatal calves on two large-scale farms in Xinjiang (Alar and Wensu) were studied using molecular biology techniques. Cryptosporidium was detected in 48.7% (185/380) of the samples and was most frequent in summer (56.

Reactive Oxygen Species Are Essential for Vasoconstriction upon Cold Exposure.

Purpose: We explored the role of ROS in cold-induced vasoconstriction and corresponding mechanism.

Methods: Three experiments were performed. First, we measured blood flow in human hands before and after cold exposure.

Protrudent Iron Single-Atom Accelerated Interfacial Piezoelectric Polarization for Self-Powered Water Motion Triggered Fenton-Like Reaction.

Water in motion presented in natural systems contains a rich source of renewable mechanical energy. Harvesting this water energy to trigger the generation of reactive oxygen species (ROS) for water purification is a desirable yet underexplored solution. Herein, the authors report a self-powered water motion triggered Fenton-like reaction system for wastewater treatment through the piezo-activation of peroxymonosulfate (PMS).

Applying Control-Value Theory and Unified Theory of Acceptance and Use of Technology to Explore Pre-service Teachers' Academic Emotions and Learning Satisfaction.

Academic emotions refer to the emotions related to achievement activities or outcomes. Academic emotions are directly related to learning performance and have been recognized as critical to learners' learning satisfaction and learning effectiveness in the online learning context. This study aimed to explore the relationship between academic emotions and learning satisfaction and their underlying mechanisms in massive open online courses (MOOCs) learning context using mediation models.

Compound Probiotics Improve Body Growth Performance by Enhancing Intestinal Development of Broilers with Subclinical Necrotic Enteritis.

The aim of this study is to explore whether or not the combined application of BS15 and H2 is capable to have a more effective control effect on SNE in broilers. A total of 240 1-day-old female chickens were randomly divided into 5 groups: (a) basal diet in negative control group (NC group); (b) basal diet + SNE infection (coccidiosis vaccine + CP) (PC group); (c) basal diet + SNE infection + H2 pre-treatment (BT group); (d) basal diet + SNE infection + BS15 pre-treatment (LT group); and (e) basal diet + SNE infection + H2 pre-treatment + BS15 pre-treatment (MT group). The results showed the MT group had the most positive effect on inhibiting the negative effect of growth performance at 42 days of age.

Methyltransferase-Deficient Avian Flaviviruses Are Attenuated Due to Suppression of Viral RNA Translation and Induction of a Higher Innate Immunity.

The 5' end of the flavivirus genome contains a type 1 cap structure formed by sequential N-7 and 2'-O methylations by viral methyltransferase (MTase). Cap methylation of flavivirus genome is an essential structural modification to ensure the normal proliferation of the virus. Tembusu virus (TMUV) (genus ) is a causative agent of duck egg drop syndrome and has zoonotic potential.

Intensification of Diabetes Medications at Hospital Discharge and Clinical Outcomes in Older Adults in the Veterans Administration Health System.

Importance: Transient elevations of blood glucose levels are common in hospitalized older adults with diabetes and may lead clinicians to discharge patients with more intensive diabetes medications than they were using before hospitalization.

Objective: To investigate outcomes associated with intensification of outpatient diabetes medications at discharge.

Design, Setting, And Participants: This retrospective cohort study assessed patients 65 years and older with diabetes not taking insulin who were hospitalized in the Veterans Health Administration Health System between January 1, 2011, and September 28, 2016, for common medical conditions.

Synchronous removal of emulsions and soluble organic contaminants via a microalgae-based membrane system: performance and mechanisms.

In this study, we applied a flexible strategy to manufacture a microalgal biochar-based membrane (MBCM). Due to the hierarchical surface topography on a micro-nano scale, the MBCM was found to have both underwater superoleophobic and underoil superhydrophobic properties. Combining an underoil superhydrophobic oil-containing region (OCR) with an underwater superoleophobic water-containing region (WCR) achieved the successive filtration of multiphase emulsions.

Student's Learning Strategies and Academic Emotions: Their Influence on Learning Satisfaction During the COVID-19 Pandemic.

Based on the control-value theory (CVT), learning strategies and academic emotions are closely related to learning achievement, and have been considered as important factors influencing student's learning satisfaction and learning performance in the online learning context. However, only a few studies have focused on the influence of learning strategies on academic emotions and the interaction of learning strategies with behavioral engagement and social interaction on learning satisfaction. The participants were 363 pre-service teachers in China, and we used structural equation modeling (SEM) to analyze the mediating and moderating effects of the data.

pH-Dependent complexation and polyelectrolyte chain conformation of polyzwitterion-polycation coacervates in salted water.

The phase behavior and chain conformational structure of biphasic polyzwitterion-polyelectrolyte coacervates in salted aqueous solution are investigated with a model weak cationic polyelectrolyte, poly(2-vinylpyridine) (P2VP), whose charge fraction can be effectively tuned by pH. It is observed that increasing the pH leads to the increase of the yielding volume fraction and the water content of dense coacervates formed between net neutral polybetaine and cationic P2VP in contrast to the decrease of critical salt concentration for the onset of coacervation, where the P2VP charge fraction is reduced correspondingly. Surprisingly, a single-molecule fluorescence spectroscopic study suggests that P2VP chains upon coacervation seem to adopt a swollen or an even more expanded conformational structure at higher pH.

Study on hyperspectral estimation model of soil organic carbon content in the wheat field under different water treatments.

Hyperspectral remote sensing technology can be used to monitor the soil nutrient changes in a rapid, real-time, and non-destructive manner, which is of great significance to promote the development of precision agriculture. In this paper, 225 soil samples were studied. The effects of different water treatments on soil organic carbon (SOC) content, and the relationship between SOC content and spectral reflectance (350-2500 nm) were studied.

Protective effect of 18β-glycyrrhetinic acid against HO-induced injury in Schwann cells based on network pharmacology and experimental validation.

The aim of the present study was to assess the protective effects of 18β-GA against hydrogen peroxide (HO)-induced injury. First, the SMILES annotation for 18β-GA was used to search PubChem and for reverse molecular docking in Swiss Target Prediction, the Similarity Ensemble Approach Search Server and the TargetNet database to obtain potential targets. Injury-related molecules were obtained from the GeneCards database and the predicted targets of 18β-GA for injury treatment were selected by Wayne diagram analysis.

Optimal model for the aircraft arrival and departure scheduling problem with fuzzy runway incursion time.

This paper presents an optimization model for assigning a set of arrival and departure flights to multiple runways and determining their actual times with consideration of incursions. Due to the lack of data, fuzzy incursion time is used to describe the uncertainty with the help of artificial experience. Moreover, the multiple-goal priority considerations of air traffic controllers are also fully considered in this model.

Simultaneous Exposure to Intracellular and Extracellular Photosensitizers for the Treatment of Staphylococcus aureus Infections.

Staphylococcus aureus is a serious threat to public health due to the rise of antibiotic resistance in this organism, which can prolong or exacerbate skin and soft tissue infections (SSTIs). Methicillin-resistant S. aureus is a Gram-positive bacterium and a leading cause of SSTIs.

Exploring the Dynamics of Week-to-Week Blood Pressure in Nursing Home Residents Before Death.

Background: Aging is accompanied by an overall dysregulation of many dynamic physiologic processes including those related to blood pressure (BP). While year-to-year BP variability is associated with cardiovascular events and mortality, no studies have examined this trend with more frequent BP assessments. Our study objective is to take the next step to examine week-to-week BP dynamics-pattern, variability, and complexity-before death.

Forward-viewing estimation of 3D blood flow velocity fields by intravascular ultrasound: Influence of the catheter on velocity estimation in stenoses.

Coronary artery disease is the most common type of cardiovascular disease, affecting > 18 million adults, and is responsible for > 365 k deaths per year in the U.S. alone.

Electroacupuncture enhances resting-state functional connectivity between dorsal caudate and precuneus and decreases associated leptin levels in overweight/obese subjects.

Electroacupuncture (EA) is a safe and effective method for treating obesity. However, how it modulates reward-related brain activity/functional connectivity and gut hormones remains unclear. We employed resting-state functional magnetic resonance imaging (RS-fMRI) and resting-state functional connectivity (RSFC) to investigate EA induced changes in resting-state activity and RSFC in reward-related regions and its association with gut hormones in overweight/obese subjects who received real (n = 20) and Sham (n = 15) stimulation.

A 3D Bioprinted In Vitro Model of Pulmonary Artery Atresia to Evaluate Endothelial Cell Response to Microenvironment.

Vascular atresia are often treated via transcatheter recanalization or surgical vascular anastomosis due to congenital malformations or coronary occlusions. The cellular response to vascular anastomosis or recanalization is, however, largely unknown and current techniques rely on restoration rather than optimization of flow into the atretic arteries. An improved understanding of cellular response post anastomosis may result in reduced restenosis.

Deprescribing Blood Pressure Treatment in Long-Term Care Residents.

Objectives: To evaluate the incidence of deprescribing of antihypertensive medication among older adults residing in Veterans Affairs (VA) nursing homes for long-term care and rates of deprescribing after potentially triggering events.

Design: Retrospective cohort study.

Setting And Participants: Long-term care residents aged 65 years and older admitted to a VA nursing home from 2006 to 2019 and using blood pressure medication at admission.