The spectrum-efficacy correlation of Kai-Xin-San for cognition of Aβ transgenic and verification of its active ingredients.

Introduction: This study aims to establish the fingerprint spectra of Kai-Xin-San (KXS) and investigate its spectrum-effect relationship in treating Alzheimer's disease (AD).

Methods: Initially, the fingerprints of 15 batches of KXS were established and analyzed using HPLC, with the method's precision, stability, and repeatability thoroughly evaluated. Subsequently, the effects of the 15 batches of KXS were assessed in an olfactory escape memory experiment, utilizing Aβ transgenic as a model.

Immunomodulatory multicellular scaffolds for tendon-to-bone regeneration.

Limited motor activity due to the loss of natural structure impedes recovery in patients suffering from tendon-to-bone injury. Conventional biomaterials focus on strengthening the regenerative ability of tendons/bones to restore natural structure. However, owing to ignoring the immune environment and lack of multi-tissue regenerative function, satisfactory outcomes remain elusive.

Xonotlite Nanowire-Containing Bioactive Scaffolds for the Therapy of Defective Adipose Tissue in Breast Cancer.

Considering the challenge in the treatment of severe breast tumor patients, xonotlite nanowire-containing bioactive scaffolds (FeO-CS-GelMA) were fabricated by the 3D-printing technique for the therapy of injured adipose tissue after surgery. Importantly, benefiting from the excellent magnetothermal performance of FeO microspheres, FeO-CS-GelMA scaffolds could effectively kill tumor cells and suppress breast cancer under an alternating magnetic field, and the tumor did not recur in 2 weeks. In addition, attributed to the released bioactive inorganic ions, FeO-CS-GelMA composite scaffolds could effectively promote the expression of adipogenesis-related genes and proteins of adipose-derived stem cells (ADSCs) via the PI3K-AKT signaling pathway .

3D multicellular micropatterning biomaterials for hair regeneration and vascularization.

Hair loss caused by the abnormal functions of hair follicles in skin can seriously impact the quality of an individual's life. The development of sophisticated skin tissue-engineered constructs is required to enable the function recovery of hair follicles. However, effective hair regrowth in skin substitutes still remains a great challenge.

Multicellular Bioprinting of Biomimetic Inks for Tendon-to-Bone Regeneration.

Tendon-to-bone interface has a hierarchical structure and gradient component that are conducive to distributing the stresses to achieve movement. Conventional biomaterials lack the capacity to induce synchronous repair of multiple tissues, resulting in the failure of the interface repair. Biomimetic strategies have attracted enormous attention in the field of complex structure regeneration because they can meet the different physiological requirements of multiple tissues.

Longevity, tumor, and physical vitality in rats consuming ginsenoside Rg1.

Background: Effects of the major ginsenoside Rg1 on mammalian longevity and physical vitality are rarely reported.

Purpose: To examine longevity, tumor, and spontaneous locomotor activity in rats consuming Rg1.

Methods: A total of 138 Wistar rats were randomized into 2 groups: control (N = 69) and Rg1 (N = 69).

An integrated supervision framework to safeguard the urban river water quality supported by ICT and models.

Models and information and communication technology (ICT) can assist in the effective supervision of urban receiving water bodies and drainage systems. Single model-based decision tools, e.g.

Inorganic-based biomaterials for rapid hemostasis and wound healing.

The challenge for the treatment of severe traumas poses an urgent clinical need for the development of biomaterials to achieve rapid hemostasis and wound healing. In the past few decades, active inorganic components and their derived composites have become potential clinical products owing to their excellent performances in the process of hemorrhage control and tissue repair. In this review, we provide a current overview of the development of inorganic-based biomaterials used for hemostasis and wound healing.

3D Printing of Diatomite Incorporated Composite Scaffolds for Skin Repair of Deep Burn Wounds.

Deep burn injury always causes severe damage of vascular network and collagen matrix followed by delayed wound healing process. In this study, natural diatomite (DE) microparticles with porous nanostructure were separated based on the particles size through a dry sieving method and combined with gelatin methacryloyl (GelMA) hydrogel to form a bioactive composite ink. The DE-containing inorganic/organic composite scaffolds, which were successfully prepared through three-dimensional (3D) printing technology, were used as functional burn wound dressings.

Preparation and characterization of a water-resistant polyamide-oxidized starch-methyl methacrylate eco-friendly wood adhesive.

A water-resistant polyamide-oxidized starch-methyl methacrylate (P-OS-M) adhesive with zero formaldehyde-emission was successfully synthesized, using natural corn starch, KMnO, polyamide and methyl methacrylate as raw material, oxidant, crosslinking agent and comonomer, respectively. The P-OS-M25 adhesive synthesized with the optimal amount of methyl methacrylate (25 ml) could reach wet shear strength of 1.04 MPa, which was far greater than natural starch (NS) and oxidized starch (OS).

Reduced stem cell aging in exercised human skeletal muscle is enhanced by ginsenoside Rg1.

Background: Stem cell aging, characterized by elevated p16 expression, decreases cell repopulating and self-renewal abilities, which results in elevated inflammation and slow recovery against stress.

Methods: Biopsied muscles were analyzed at baseline and 24 h after squat exercise in 12 trained men (22 ± 2 y). Placebo (PLA) and immunostimulant Rg1 (5 mg) were supplemented 1 h before a squat exercise, using a double-blind counterbalanced crossover design.

3D Printing of Strontium Silicate Microcylinder-Containing Multicellular Biomaterial Inks for Vascularized Skin Regeneration.

The reconstruction of dermal blood vessels is essential for skin regeneration process. However, the lack of vascular structure, insufficient angiogenesis induction, and ineffective graft-host anastomosis of the existing skin substitutes are major bottle-necks for permanent skin replacement in tissue engineering. In this study, the uniform strontium silicate (SS) microcylinders are successfully synthesized and integrated into the biomaterial ink to serve as stable cell-induced factors for angiogenesis, and then a functional skin substitute based on a vascularization-induced biomimetic multicellular system is prepared via a "cell-writing" bioprinting technology.

Aerobic exercise induces tumor suppressor p16 expression of endothelial progenitor cells in human skeletal muscle.

Aerobic exercise induces oxidative stress and DNA damage, nevertheless, lowers cancer incidence. It remains unclear how genetic stability is maintained under this condition. Here, we examined the dynamic change of the tumor suppressor p16 in cells of skeletal muscle among young men following 60-min of aerobic cycling at 70% maximal oxygen consumption (V̇O).

Black Bioceramics: Combining Regeneration with Therapy.

Bioceramics have been developed from bioinert to bioactive or biodegradable materials in the past few decades. However, at present, traditional bioceramics are still mainly used in bone tissue regeneration and dental restoration. In this work, a new generation of "black bioceramics," extending the applications from tissue regeneration to disease therapy, is presented.

Lower tumorigenesis without life extension in rats receiving lifelong deep ocean minerals.

Naturally occurring tumor in animals receiving high minerals from deep oceans (DOM: hardness 600 mg/L) from 6 months of age until natural death was firstly assessed in 200 Sprague Dawley rats, randomized into four groups: Control (C), DOM (D), Fructose (F), and Fructose + DOM (FD). Fructose drink contained 11% fructose. Tumor incidence (necropsy at death) in the D group was ~40% lower than that in the C group (P < .

Contrasting actions of ginsenosides Rb1 and Rg1 on glucose tolerance in rats.

Ginsenoside profile of Panax ginseng is changing with season and cultivated soil. Yet, dose-response relationship of main ginsenosides on metabolic measures has not been documented in vivo. Here, we examined glucose and insulin responses after an oral glucose challenge (0.

Ginsenoside Rg1 supplementation clears senescence-associated β-galactosidase in exercising human skeletal muscle.

Background: Ginsenoside Rg1 has been shown to clear senescence-associated beta-galactosidase (SA-β-gal) in cultured cells. It remains unknown whether Rg1 can influence SA-β-gal in exercising human skeletal muscle.

Methods: To examine SA-β-gal change, 12 young men (age 21 ± 0.

Defective Black Nano-Titania Thermogels for Cutaneous Tumor-Induced Therapy and Healing.

Current challenges in cutaneous tumor therapy are healing the skin wounds resulting from surgical resection and eliminating possible residual tumor cells to prevent recurrence. To address this issue, bifunctional biomaterials equipped with effective tumor therapeutic capacity for skin cancers and simultaneous tissue regenerative ability for wound closure are highly recommended. Herein, we report an injectable thermosensitive hydrogel (named BT-CTS thermogel) with the integration of nanosized black titania (B-TiO, ∼50 nm) nanoparticles into a chitosan (CTS) matrix.

Deep Ocean Minerals Minimize Eccentric Exercise-Induced Inflammatory Response of Rat Skeletal Muscle.

We have previously shown an accelerated recovery from muscle fatigue in men challenged by prolonged exercise after oral deep ocean minerals (DOM) supplementation. Here, we hypothesized a decrease in eccentric exercise-induced muscle inflammation in rats regularly consuming DOM-containing drinks (hardness 600 mg/L and fructose 11%). Forty-seven male Sprague Dawley rats were randomized into 4 groups: Control (C, = 12), Fructose (F, = 12), Fructose+Exercise (FE, = 12), and Fructose+Exercise+DOM (FED, = 11).

Aged cells in human skeletal muscle after resistance exercise.

Unlabelled: It remains unclear how exercise, as an entropic event, brings benefit against human aging. Here we examined longitudinal changes of p16 senescent cells in skeletal muscle of young men (aged 22.5±1.

Fasudil preserves lung endothelial function and reduces pulmonary vascular remodeling in a rat model of end‑stage pulmonary hypertension with left heart disease.

Pulmonary hypertension (PH) due to left heart disease (LHD) is a common condition associated with significant morbidity. It contributes to the elevation of pulmonary vascular resistance and mean pulmonary pressure, eventually leading to heart failure and even mortality. The present study aimed to explore the potential efficacy of late and long‑term treatment with a Rho‑kinase (ROCK) signaling inhibitor, namely fasudil, in a rat model of end‑stage PH‑LHD.

Doxorubicin inhibits muscle inflammation after eccentric exercise.

Background: Doxorubicin, a widely used anti-tumour drug, is known to cause muscle loss in cancer patients.

Methods: Following an acute dose of doxorubicin injection (2.5 mg/kg per body weight), we examined macrophage distribution in rat soleus muscle challenged by eccentric exercise (downhill running).

Study on the Overexpression of the Gene Encoding Arginyl-tRNA Synthetase under Induction.

Arginyl-tRNA synthetase (ArgRS) from E. coli was overproduced from transformant containing the gene encoding this enzyme (argS) by 550 fold higher than that from host cell. By site-directed mutagenesis the cleavage site for NcoI restriction endonuclease was introduced into the start codon of argS.

Gene Cloning, Overproduction and Purification of Escherichia coli tRNA(Arg)(2).

A synthetic gene encoding Escherichia coli tRNA(Arg)(2) was inserted in a plasmid under the control of an isopropyl-beta, D-thiogalactopyranoside (IPTG)-inducible promotor, pTrc99B. In E.coli MT102 transformed by the above plasmid containing the target gene.