A LncRNA-miRNA-mRNA ceRNA regulatory network based tuberculosis prediction model.

The high incidence of tuberculosis (TB) has brought serious social burdens and it is urgent to explore the mechanism of TB development. This study was conducted to analyze the role of lncRNA-miRNA-mRNA regulatory network and its contained nodes involved in TB to identify crucial biomarkers for early diagnosis of TB. Long-noncoding RNAs (lncRNAs), messenger RNA (mRNAs) and microRNAs (miRNAs) expression profiles of TB patients and healthy individuals were downloaded from the GSE34608 dataset.

Incidence of death from kidney diseases among cancer patients: a US population-based analysis.

Background: The purpose of our study was to retrospectively analyze the characteristic of death from kidney diseases among cancer patients and to screen the risk factors associated with nephrotic death using data from the surveillance, epidemiology, and end results (SEER) database.

Methods: The information on cancer patients dying of kidney diseases was retrieved from the SEER database. Standardized mortality ratios (SMRs) were calculated using the US general population as reference.

The effects of HIF-1α overexpression on renal injury, immune disorders and mitochondrial apoptotic pathways in renal ischemia/reperfusion rats.

Background: Renal ischemia/reperfusion (RI/R) injury are a common pathogenesis of acute kidney injury, which may cause renal parenchyma damage clinically. Hypoxia-inducible factor-1α (HIF-1α) has protective effects on cells in regulating the metabolism, angiogenesis, erythropoiesis, and anti-apoptosis of RI/R injury. However, the specific mechanisms for HIF-1α on RI/R injury are still unclear.

Levels of tetrabromobisphenol A, hexabromocyclododecanes and polybrominated diphenyl ethers in human milk from the general population in Beijing, China.

Three brominated flame retardants (BFRs), tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) and polybrominated diphenyl ethers (PBDEs), were measured in 103 human milk samples collected from Beijing in 2011. The donors' personal information, such as dietary habit and socioeconomic and lifestyle factors, was obtained by questionnaires. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) analysis indicated that the levels of TBBPA ranged from View Article and Find Full Text PDF

Soy isoflavone attenuates brain mitochondrial oxidative stress induced by β-amyloid peptides 1-42 injection in lateral cerebral ventricle.

The aim of this study is to investigate whether soy isoflavone (SIF) reduces oxidative stress and improves the antioxidant ability in mitochondria of rat brain damaged by injection of beta-amyloid peptides 1-42 (Aβ1-42). Forty Wistar rats were randomly divided into control, Aβ1-42, SIF + Aβ1-42, and SIF groups according to body weight. The rats in the SIF + Aβ1-42 group and SIF group were intragastrically administered SIF suspension in 0.

Validation of the use of the ROSIER scale in prehospital assessment of stroke.

Aim: To determine the utility of the Recognition of Stroke in the Emergency Room (ROSIER) scale as a stroke recognition tool among Chinese patients in the prehospital setting.

Materials And Methods: Compared with the Cincinnati Prehospital Stroke Scale (CPSS), emergency physicians prospectively used the ROSIER as a stroke recognition tool on suspected patients in the prehospital setting. And, the final discharge diagnosis of stroke or transient ischemic attack made by neurologists, after assessment and review of clinical symptomatology and brain imaging findings, was used as the reference standard for diagnosis in the study.

Antagonizing effects of soybean isoflavones on β-amyloid peptide-induced oxidative damage in neuron mitochondria of rats.

Soybean isoflavone (SIF) has been demonstrated to have neuroprotective effects induced by β-amyloid peptides (Aβ) through suppressing oxidative stress; however, the explicit mechanisms still remain uncovered. In the present study, 32 Wistar rats were randomly divided into four groups: an Aβ1-42-treated group, a SIF + Aβ1-42 group, a SIF-treated group and a control group. We measured the protein content of 8-hydroxydeoxyguanosine (8-OhdG) and mRNA expression of 8-oxoguanine DNA glycosylase (OGG1).

[Determination of three brominated flame retardants in human serum using solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry].

A solid-phase extraction (SPE) method for the simultaneous extraction of hexabromocyclododecanes (HBCDs)/tetrabromobisphenol A (TBBPA) and polybrominated diphenyl ethers (PBDEs) in human serum was developed. The extracts of HBCDs/TBBPA and PBDEs were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-negative chemical ionization/mass spectrometry (GC-NCI/MS), respectively. The samples with the spiked internal standards, 13C(12)-HBCD, 13C(12)-TBBPA, 3, 3', 4, 4'-tetrabromodiphenyl ether (BDE-77) and 13C(12)-decabromodiphenyl ether (BDE-209), were extracted using the mixture of methyl tert-butyl ether (MTBE) and hexane (1:1, v/v).

Flavonoids protect cerebrovascular endothelial cells through Nrf2 and PI3K from β-amyloid peptide-induced oxidative damage.

β-amyloid peptides (Aβ) induced cerebrovascular dysfunction has been recognized as a vital factor involved in the pathogenesis of neurodegeneration. Genistein, a flavonoid, has antioxidative properties to prevent neurodegeneration induced by β-amyloid peptides. In this study, we were investigating whether genistein could antagonize oxidative damage induced by β-amyloid peptide 25-35 (Aβ25-35) in bEND.

[Determination of polybrominated diphenyl ethers and dechlorane plus in fish and fish oil supplements by gel permeation hromatography coupled with gas chromatography-negative chemical ionization mass spectrometry].

Polybrominated diphenyl ethers (PBDEs) and dechlorane plus (DP) are two kinds of widely used organic flame retardants, and PBDE and DP residues have been found in both environment and biota. A method for the determination of 8 PBDEs and 2 DPs in fish and fish oil supplements was developed using auto gel permeation chromatography (GPC) coupled with gas chromatography-negative chemical ionization/mass spectrometry (GC-NCI/MS) in the selected ion-monitoring (SIM) mode on a 15 m capillary column. The sample added with BDE-77 and 13C12-BDE-209 as the internal standards was extracted using a Soxhlet extractor, and further purified by auto GPC and a multilayer silica gel column.

The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects.

The correlation of glutathione S-transferase (GST) M1/T1 genetic polymorphisms with oxidative stress-related chronic diseases was proved recently. The aim of the present study was to investigate the association of GSTM1/T1 genetic polymorphisms with antioxidant biomarkers and consumption of fruits and vegetables (F&V) in healthy subjects. In this study, for conducting a 3 d dietary survey, 190 healthy adults were recruited.

Genistein inhibits mitochondrial-targeted oxidative damage induced by beta-amyloid peptide 25-35 in PC12 cells.

The antioxidative properties of genistein (Gen) have been demonstrated by our previous studies and others, but its potential mechanism was not very clear. Because of the key role of mitochondria in oxidant production, we wondered if mitochondria were one of Gen's neuroprotective targets. In the present study we investigated whether Gen has protective effects on mitochondria damaged by Aβ25-35.

Genistein and folic acid prevent oxidative injury induced by β-amyloid peptide.

To explore the mechanism(s) of the neuroprotective effects of genistein (GEN) and folic acid (FA) on neurons treated with beta amyloid 31-35 (Aβ31-35), the primary cultured cortical neurons were treated with GEN and/or FA for 2 hr prior to exposure to Aβ31-35. Cell viability and fluidity of cell membrane were measured by 3-[4,5-dimethylthiazol-2]-2,5 diphenyltetrazolium bromide and fluorescence polarization, respectively. Intracellular reactive oxygen species (ROS) and Ca(2+) concentrations were measured by laser scanning confocal microscope.

[Effects of genistein and folic acid on neuronal membrane and mitochondrial membrane damaged by β-amyloid peptides 31-35].

Objective: To observe the neuro-protective effects of genistein (Gen) and folic acid (FA) on neurons membrane and mitochondrial membrane damaged by β-amyloid peptides 31-35 (Aβ31-35).

Methods: The primary cultured rat cerebral cortical neurons were randomly divided into DMEM (control), Aβ31-35 (25 µmol/L), Gen (Gen 27 µg/ml), FA (FA 40 µg/ml) and Gen + FA (Gen 27 µg/ml + FA 40 µg/ml). Gen and/or FA were added two hours before Aβ31-35 addition.

Genistein as a neuroprotective antioxidant attenuates redox imbalance induced by beta-amyloid peptides 25-35 in PC12 cells.

Objective: Genistein (GEN), a principal component of soybean isoflavones, might possess the neuroprotective role through its antioxidant activity. However, the detailed mechanisms are unknown yet. The purpose of this study was to investigate whether GEN could alleviate oxidative damage induced by beta-amyloid peptides 25-35 (Abeta25-35) in PC12 cells.

Long-term effects of high lipid and high energy diet on serum lipid, brain fatty acid composition, and memory and learning ability in mice.

Objective: It is well known that high lipid and high energy diet is harmful to health. But the different effects of high lipid diet composed of either saturated fatty acids or unsaturated fatty acids have not been distinguished.

Method: Eighteen pregnant C57BL/6j (22-25g) mice were randomly divided into three groups of six each and fed with chow or high lipid diet composed of either flaxseed oil (chow diet 84%, cholesterol 0.

Dietary exposure assessment of Chinese adults and nursing infants to tetrabromobisphenol-A and hexabromocyclododecanes: occurrence measurements in foods and human milk.

Tetrabromobisphenol A(TBBPA) and hexabromocyclododecane diastereoisomers (alpha, beta, and gamma-HBCD) were determined in 24 pooled human milk samples and 48 Chinese total diet study (TDS) samples collected in 2007. On the basis of ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) analysis, levels of TBBPA ranged from < LOD to 5124 pg/g lipid weight (lw) in human milk and from < LOD to 2044 pg/g lw in TDS samples. The alpha-HBCD diastereoisomer, which ranged from < LOD to 2776 pg/g lw in human milk and from < LOD to 2224 pg/g lw in TDS samples, was generally the most abundant isomer comparing with beta- and gamma-HBCD.

Neuroprotective effects of genistein and folic acid on apoptosis of rat cultured cortical neurons induced by beta-amyloid 31-35.

Genistein and folic acid have been reported respectively to protect against the development of cognitive dysfunction; however, the underlying mechanism(s) for this protection remain unknown. In this report, the mechanism(s) contributing to the neuroprotective effects of genistein and folic acid were explored using rat cortical neuron cultures. We found that genistein and folic acid, both separately and collaboratively, increased cell viability and mitochondrial membrane potential in beta-amyloid (Abeta) 31-35-treated neurons.

[Analysis of hexabromocyclododecane diastereoisomers in foods of animal origin using ultra performance liquid chromatography-mass spectgrometry and isotope dilution].

A method for the detection of the alpha, beta and gamma-diastereoisomers of hexabromocyclododecane (HBCDs) in foods of animal origin, such as fish, chick, milk, butter etc., was developed using ultra performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESI-MS) and isotope dilution. The HBCDs with the spiked isotopic 13C-HBCDs, the internal standards, were extracted using Soxhiet extraction, and further purified using acidic silica treatment and solid phase extraction.

[Lead and cadmium pollution in edible fungus sold in Beijing].

Objective: To investigate the lead and cadmium pollution in edible mushrooms sold in Beijing.

Methods: 146 samples of 14 species were collected form 25 markets during the period of Mar. through May, 2007 in Beijing.

[Contamination source identification study of organochlorines pesticides in 2000 Chinese total diet study].

The third Chinese total diet study to estimate dietary intake of contaminants was carried out in 2000. The levels of HCH and DDT residues were less significant in most of foodstuffs, but the level of HCH residues of aquatic foods in South 2 and in North 1 is respectively 75.99microg/kg and 170.