Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.

Background: Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases, is characterized by the presence of numerous fluid-filled renal cysts and is a leading cause of end-stage renal disease (ESRD). Urinary biomarkers may be useful for predicting the variable course of ADPKD progression from cyst growth to ESRD.

Methods: To identify candidate urinary biomarkers of ADPKD progression, we used CRISPR/Cas9 genome editing to generate porcine fibroblasts with mono- and biallelic ADPKD gene knockout (PKD2+/- and PKD2-/-, respectively).

Expression and secretion of CXCL12 are enhanced in autosomal dominant polycystic kidney disease.

Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases (frequency of 1/1000-1/400), is characterized by numerous fluid-filled renal cysts (RCs). Inactivation of the PKD1 or PKD2 gene by germline and somatic mutations is necessary for cyst formation in ADPKD. To mechanistically understand cyst formation and growth, we isolated RCs from Korean patients with ADPKD and immortalized them with human telomerase reverse transcriptase (hTERT).

The complete mitogenome of a peanut worm (Sipuncula, Phascolosomatida, Phascolosomatidea).

Sipuncula (peanut worms) is a traditional phylum which consists of unsegmented bilateral symmetric marine worms. Although it is accepted as a phylum, the phylogenetic position of Sipuncula has been questionable. There is lack of molecular record on Sipuncula species while morphological data is not enough to distinguish the closer relationships between Annelida and Sipuncula.

Complete mitochondrial genome analysis of sp. (Sipuncula, Phascolosomatida, Phascolosomatidea) from Micronesia.

In this study a Sipuncula species sp. was collected from seagrass area from Chuuk lagoon Micronesia and its complete mitochondrial genome analyzed. This is the second complete mitochondrial genome record from the genus after .

Complete mitochondrial genome analysis of (Baba, 1937) (Mollusca, Gastropoda, Nudibranchia).

In this study, a complete mitochondrial genome of a nudibranch species, was sequenced and analyzed. The mitochondrial genome size is 15,059 bp with 28.1% A, 14.

Complete mitochondrial genome of (Decapoda, Xanthidae).

The complete mitochondrial genome sequenced from a xanthid crab, which was collected from a rocky intertidal area in Chuuk lagoon. The mitochondrial genome size of evaluated as 15,480 bp with 33.6% A, 24% C, 11.

The complete mitochondrial genome of (Gastropoda, Neogastropoda, Muricidae) collected from Chuuk Lagoon.

Complete mitochondrial genome of the knobbed rock shell sea snail (Röding, 1798) has been sequenced and phylogenetic relationships evaluated due to mitochondrial protein coding genes. The size of mitochondrial genome for is 15,294 bp and the nucleotide composition of the mitochondrial genome is 28.4% A, 16.

The complete mitochondrial genome of (Herbst, 1783) (Decapoda, Grapsidae).

A complete mitochondrial genome was sequenced from a grapsid crab, (Herbst 1783), which was collected from a rocky intertidal zone of Chuuk lagoon. The size of mitochondrial genome is 15,858 bp with 31.9% A, 22.

Analysis of complete mitochondrial genome of (Latreille, 1818) (Decapoda, Ocypodidae).

Complete mitochondrial genome of smooth-handed ghost crab (Latreille, 1818) has been sequenced and phylogenetic relationships evaluated due to mitochondrial protein coding genes. This is the second record of complete mitochondrial genome from the genus. The size of mitochondrial genome for is 15,604 bp and the nucleotide distribution of the mitochondrial genome is 31.

Complete sequences of mitochondrial genome of (A. Adams, 1861) (Mollusca, Gastropoda, Nudibranchia).

In this study, complete nucleotide sequences of the mitochondrial genome of a nudibranch species, (A. Adams, 1861) were determined and characterized. The mitogenome size is 14 880 bp.

Complete mitochondrial genome sequences and phylogenetic relationship of (Swainson, 1840) (Mollusca, Gastropoda, Heterobranchia, Sacoglossa).

The sacoglossans are small sea slugs in the Heterobranchia. In this study, mitochondrial genome of a sacoglossan sea slug, (Swainson, 1840) is analyzed and phylogenetic tree of the species in the Heterobranchia is reconstructed based on amino acid sequences of mitochondrial protein coding genes. The phylogenetic relationship analysis shows that belongs to the monophyletic Sacoglossa in the Heterobranchia and the Sacoglossa shows sister group relationship with a lineage including Anaspidea and Siphonarioidea.

A heuristic for disassembly planning in remanufacturing system.

This study aims to improve the efficiency of disassembly planning in remanufacturing environment. Even though disassembly processes are considered as the reverse of the corresponding assembly processes, under some technological and management constraints the feasible and efficient disassembly planning can be achieved by only well-designed algorithms. In this paper, we propose a heuristic for disassembly planning with the existence of disassembled part/subassembly demands.

An adaptive evolutionary algorithm for traveling salesman problem with precedence constraints.

Traveling sales man problem with precedence constraints is one of the most notorious problems in terms of the efficiency of its solution approach, even though it has very wide range of industrial applications. We propose a new evolutionary algorithm to efficiently obtain good solutions by improving the search process. Our genetic operators guarantee the feasibility of solutions over the generations of population, which significantly improves the computational efficiency even when it is combined with our flexible adaptive searching strategy.

Genomic imbalances in Korean hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is one of the most prevalent gastrointestinal malignant tumors in Southeast Asia. Thirty-one cirrhotic HCC, 14 noncirrhotic HCC, and 13 metastastic HCC in the Korean population were investigated on microdissected tissues for chromosomal aberrations by degenerate oligonucleotide-primed polymerase chain reaction (PCR) comparative genomic hybridization. A number of prominent sites of genomic imbalances were observed.