"Pulsed" Chain Transfer Anionic Polymerization: A Green and Cost-Effective Approach for Controlled Polymer Synthesis.

Herein, we report a method that significantly enhances the atom economy of catalysts in living polymerization. By introducing intermittent, switchable chain-transfer reactions during the polymerization process, multiple regenerations of the catalyst are achieved while preserving the "living"/controlled characteristics. The specially designed catalytic system, composed of 1,2-dipyrrolidinoethane/n-butyllithium/n-butylpotassium/toluene, exhibits a chain-transfer rate that is highly sensitive to temperature.

Recent advances in the detection of microplastics in the aqueous environment by electrochemical sensors: A review.

Microplastics (MPs), as an emerging contaminant, have become a serious threat to marine ecosystems due to their small size, widespread distribution and easy ingestion by organisms. Therefore, it is necessary to develop various analytical techniques to detect MPs in real water environment. Among these detection techniques, the advantages of electrochemical sensors, such as easy operation, high sensitivity and low cost, provide the possibility of online real-time detection of MPs in real water environment.

Effectiveness of perioperative remimazolam in preventing postoperative delirium: a systematic review and meta-analysis.

Background: To compare the POD rates in patients undergoing non-cardiac surgery who received remimazolam perioperatively versus placebo or other sedatives.

Methods: We systematically searched four major databases (Cochrane Central Register of Controlled Trials, Web of Science, Embase, and PubMed) for relevant randomized controlled trials (RCTs) up to July 11, 2024. Literature quality evaluation was used the bias risk table in Review Manager 5.

Senescence-specific molecular subtypes stratify the hallmarks of the tumor microenvironment and guide precision medicine in bladder cancer.

Background: Bladder cancer (BLCA) is notably associated with advanced age, characterized by its high incidence and mortality among the elderly. Despite promising advancements in models that amalgamate molecular subtypes with treatment and prognostic outcomes, the considerable heterogeneity in BLCA poses challenges to their universal applicability. Consequently, there is an urgent need to develop a new molecular subtyping system focusing on a critical clinical feature of BLCA: senescence.

Single-Cell/Particle Sample Introduction Device for Mass Cytometry Based on Gas-Driven Flow Focusing.

Mass cytometry is considered the second generation of flow cytometry technology, which uses metal-labeled antibodies instead of traditional fluorescent antibodies and can measure much more markers from a single cell simultaneously. However, due to frequent clogging of the capillary and nebulizer, it is still a complicated task to perform the sample introduction smoothly for a long-term determination. Herein, a plug-and-play sample introduction device for mass cytometry analysis was developed based on a gas-driven flow focusing protocol.

Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea.

Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.

Study on the Transformation Process of Thyroid Fine-Needle Aspiration Liquid-Based Cytology to Whole-Slide Image.

Objective: Analyse and summarise the reasons for failure in the digital acquisition of thyroid liquid-based cytology (LBC) slides and the technical challenges, and explore methods to obtain reliable and reproducible whole digital slide images for clinical thyroid cytology.

Method: Use the glass slide scanning imaging system to acquire whole-slide image (WSI) of thyroid LBC in sdpc format through different. Statistical analysis was conducted on the different acquisition methods, the quality of the glass slides, clinical and pathological characteristics of the case, TBSRTC grading and the quality of WSI.

Process-based quantitative description of carbon biogeochemical cycle in a reclaimed water intake area.

Reclaimed water plays a pivotal role in addressing water scarcity and pollution. The carbon (C) cycle significantly impacts aquatic ecosystems and water quality, yet the C biogeochemical cycle in nutrient-rich reclaimed water remains enigmatic. This study focuses on reclaimed water, developing a conceptual biogeochemical mass balance model to examine C cycling and assess the C budget in the highly eutrophic Jian and Chaobai rivers.

Methionine-driven YTHDF1 expression facilitates bladder cancer progression by attenuating RIG-I-modulated immune responses and enhancing the eIF5B-PD-L1 axis.

The impact of amino acids on tumor immunotherapy is gradually being uncovered. In this study, we screened various essential and non-essential amino acids and found that methionine enhances mRNA methylation and reduced the activation of Type I interferon pathway in bladder cancer. Through RNA sequencing, point mutations, MB49 mouse tumor models, and single-cell RNA sequencing, we demonstrated that high methionine levels elevate the expression of mA reader YTHDF1, promoting the degradation of RIG-I, thereby inhibiting the RIG-I/MAVS-mediated IFN-I pathway and reducing the efficacy of tumor immunotherapy.

Evasion of immunosurveillance by the upregulation of Siglec15 in bladder cancer.

Immunotherapy resistance in bladder cancer (BLCA) is associated with elevated levels of sialic acid-binding immunoglobulin-like lectin (Siglec15). This protein plays a crucial role in fostering a noninflammatory tumor microenvironment (TME), which is conducive to cancer progression. Our study confirmed that the overexpression of Siglec15 led to a reduction in CD8 T cell infiltration.

Insights into the historical trajectory and research trends of immune checkpoint blockade in colorectal cancer: visualization and bibliometric analysis.

Background: Colorectal cancer (CRC) is a malignant tumor that poses a significant threat to human health due to rising incidence and mortality rates. In recent years, immune checkpoint blockade (ICB) therapy, represented by Programmed death receptor 1 (PD-1), T-lymphocyte-associated protein 4 (CTLA-4), and others, has been widely applied in CRC and has achieved encouraging results in some patients and has become a hot topic in both clinical and basic research.

Objective: This study undertakes a comprehensive bibliometric analysis of ICB research in CRC, aiming to evaluate the current status, identify future trends, and provide scientific insights for researchers and decision-makers.

Developing hypoxia and lactate metabolism-related molecular subtypes and prognostic signature for clear cell renal cell carcinoma through integrating machine learning.

Background: The microenvironment of clear cell renal cell carcinoma (ccRCC) is characterized by hypoxia and increased lactate production. However, the impact of hypoxia and lactate metabolism on ccRCC remains incompletely understood. In this study, a new molecular subtype is developed based on hypoxia-related genes (HRGs) and lactate metabolism-related genes (LMRGs), aiming to create a tool that can predict the survival rate, immune microenvironment status, and responsiveness to treatment of ccRCC patients.

Hybrid Carbon Black/Silica Reinforcing System for High-Performance Green Tread Rubber.

Silica, as a high-quality reinforcing filler, can satisfy the requirements of high-performance green tread rubber with high wet-skid resistance, low rolling resistance, and low heat generation. However, the silica surface contains abundant silicon hydroxyl groups, resulting in a severe aggregation of silica particles in non-polar rubber matrix. Herein, we explored a carbon black (CB)/silica hybrid reinforcing strategy to prepare epoxidized natural rubber (ENR)-based vulcanizates.

[Stabilization of Cd-contaminated Soil with Thiol-modified Biochar and Response of Soil Microorganisms].

To explore the stabilization effect of livestock manure biochar on Cd-contaminated soil and its impact on the soil environment, a pot experiment was conducted to investigate the stabilization efficiency of cattle manure-biochar （BC） and thiol-modified biochar （SBC） on Cd in soil and their effect on the soil properties and microbial community. The structural equation model （SEM） was used to analyze the effect pathways of BC and SBC on the soil microbial community. The results showed that BC and SBC increased soil pH, available potassium, available phosphorus, and organic matter content but decreased soil available nitrogen content compared with those in CK.

Coordination engineering of single-atom ruthenium in 2D MoS for enhanced hydrogen evolution.

This study investigates the enhancement of catalytic activity in single-atom catalysts (SACs) through coordination engineering. By introducing non-metallic atoms (X = N, O, or F) into the basal plane of MoS defect engineering and subsequently anchoring hetero-metallic Ru atoms, we created 10 types of non-metal-coordinated Ru SACs (Ru-X-MoS). Computations indicate that non-metal atom X significantly modifies the electronic structure of Ru, optimizing the hydrogen evolution reaction (HER).

Unveiling the genetic basis and metabolic rewiring behind the galactose-positive phenotype in a Streptococcus thermophilus mutant.

Streptococcus thermophilus (S. thermophilus) is a widely used starter culture in dairy fermentation, but most strains are galactose-negative and only metabolize glucose from lactose hydrolysis. In this study, we aimed to uncover the mechanisms underlying the acquisition of a stable galactose-positive (Gal) phenotype in a mutant strain of S.

Dichloroacetate Prevents Sepsis Associated Encephalopathy by Inhibiting Microglia Pyroptosis through PDK4/NLRP3.

Dichloroacetate (DCA), a pyruvate dehydrogenase kinase inhibitor, is often used to treat lactic acidosis and malignant tumors. Increasing studies have shown that DCA has neuroprotective effects. Here, we explored the role and mechanism of DCA in Sepsis associated encephalopathy (SAE).

Fe-Catalyzed Fluorosulfonylation of Alkenes via Sulfur Dioxide Insertion: Synthesis of Lactam-Functionalized Alkyl Sulfonyl Fluorides.

A novel Fe-catalyzed fluorosulfonylation of alkenes with NaSO and -fluorobenzenesulfonimide (NFSI) for assembling various lactam-functionalized alkyl sulfonyl fluorides is disclosed. In this reaction, NaSO acts as both an SO source and a reductant. Furthermore, the resulting products can be efficiently transformed into valuable chemicals, including sulfonyl esters and sulfonamides, via the sulfur(VI) fluoride exchange (SuFEx) click reaction.

5-Fluorouracil resistance-based immune-related gene signature for COAD prognosis.

Background: Drug resistance is the primary obstacle to advanced tumor therapy and the key risk factor for tumor recurrence and death. 5-Fluorouracil (5-FU) chemotherapy is the most common chemotherapy for individuals with colorectal cancer, despite numerous options.

Methods: The Gene Expression Omnibus database was utilized to extract expression profile data of HCT-8 human colorectal cancer wild-type cells and their 5-FU-induced drug resistance cell line.