High-expression of Galactosidase alpha is correlated with poor prognosis and immune infiltration in low-grade glioma.

Galactosidase alpha (GLA), a member of galactosidase (GAL) family, contributes to cancer diagnosis and targeted therapy. Up to now, neither prognosis nor immune infiltration has been demonstrated in cases with low-grade glioma (LGG). In LGG, we investigated the association between GLA expression and immune infiltration levels.

A systematic study of traditional Chinese medicine treating hepatitis B virus-related hepatocellular carcinoma based on target-driven reverse network pharmacology.

Hepatocellular carcinoma (HCC) is a serious global health problem, and hepatitis B virus (HBV) infection remains the leading cause of HCC. It is standard care to administer antiviral treatment for HBV-related HCC patients with concurrent anti-cancer therapy. However, a drug with repressive effects on both HBV infection and HCC has not been discovered yet.

ABCD3 is a prognostic biomarker for glioma and associated with immune infiltration: A study based on oncolysis of gliomas.

Background: Gliomas are the most lethal primary brain tumors and are still a major therapeutic challenge. Oncolytic virus therapy is a novel and effective means for glioma. However, little is known about gene expression changes during this process and their biological functions on glioma clinical characteristics and immunity.

Anomalously low friction of confined monolayer water with a quadrilateral structure.

In this work, we explored how the structure of monolayer water confined between two graphene sheets is coupled to its dynamic behavior. Our molecular dynamics simulations show that there is a remarkable interrelation between the friction of confined water with two walls and its structure under extreme confinement. When the water molecules formed a regular quadrilateral structure, the friction coefficient is dramatically reduced.

A Comprehensive Method for Accurate Strain Distribution Measurement of Cell Substrate Subjected to Large Deformation.

Cell mechanical stretching is a fundamental technique commonly used in cardiovascular mechanobiology research. Accordingly, it is crucial to measure the accurate strain field of cell substrate under different strains. Digital image correlation (DIC) is a widely used measurement technique, which is able to obtain the accurate displacement and strain distribution.

[Effect of Biaxial Tensile Strain on Expression of Osteogenic Specificity Markers of Rat Bone Marrow-derived Mesenchymal Stem Cells in Vitro].

The purpose of this study was to investigate the effect of biaxial tensile strain on the osteogenic differentiation of rat bone marrow mesenchymal stem cells(rBMSCs)in vitro.The rBMSCs were isolated from tibia and femur of 4weeks-old Sprague-Dawley(SD)rats.The rBMSCs were cultured in DMEM-LG complete culture medium and grew to subconfluence in the cell culture device for loading tensile strain.

Physiological pulsatile flow culture conditions to generate functional endothelium on a sulfated silk fibroin nanofibrous scaffold.

Many studies have demonstrated that in vitro shear stress conditioning of endothelial cell-seeded small-diameter vascular grafts can improve cell retention and function. However, the laminar flow and pulsatile flow conditions which are commonly used in vascular tissue engineering and hemodynamic studies are quite different from the actual physiological pulsatile flow which is pulsatile in nature with typical pressure and flow waveforms. The actual physiological pulsatile flow leading to temporal and spatial variations of the wall shear stress may result in different phenotypes and functions of ECs.

[Apoptosis of A549 cells induced by cloned Noxa gene].

Objective: To clone the Noxa gene and to observe the apoptosis of A549 cells transfected with the recombinant plasmid of pcDNA-Noxa.

Methods: The Noxa gene was obtained by PCR, and was cloned into pcDNA3. 1(-).

[Comparison of soil fertility among open-pit mine reclaimed lands in Antaibao regenerated with different vegetation types].

Re-vegetation is mainly applied into regeneration in opencast mine to improve the soil quality. It is very important to choose feasible vegetation types for soil restoration. In this study, three typical forest restoration types were studied at Antaibao mine, namely, Medicago sativa, mixed forests Pinus taebelaefolius-Robinia pseudoacacia-Caragana korshinskii and Elaeagnus angustifolia-Robinia pseudoacacia-Caragana korshinskii-Hipophae rhamnoides, to determine the nutrient contents and enzyme activities in different soil layers.

Characterization of pLP18, a novel cryptic plasmid of Lactobacillus plantarum PC518 isolated from Chinese pickle.

A cryptic plasmid of Lactobacillus plantarum PC518 isolated from Chinese pickle, designated pLP18, was sequenced and characterized. It is a 1806-bp circular molecule with a G+C content of 37.5%.

Functional identification of a putative beta-galactosidase gene in the special lac gene cluster of Lactobacillus acidophilus.

The putative beta-galactosidase gene (lacZ) of Lactobacillus acidophilus has a very low degree of homology to the Escherichia coli beta-galactosidase gene (lacZ) and locates in a special lac gene cluster which contains two beta-galactosidase genes. No functional characteristic of the putative beta-galactosidase has been described so far. In this study, the lacZ gene of L.

[Cloning, expression and characterization of a heterodimeric beta-galactosidase from Lactobacillus acidophilus ATCC 4356].

Unlabelled: Heterodimeric beta-galactosidase of Lactobacillus acidophilus belongs to glycoside hydrolase family 2, encoded by two overlapping and translational coupling genes, lacL and lacM. The lacL and lacM genes of the sequenced strain L. acidophilus NCFM encode polypeptides with calculated molecular masses of 73,253 and 35,817 Da, respectively.

[Measuring volume and density of human organs and its significance in medicine and forensic medicine: brain and heart part].

The exact scales of volume and density of human internal organs were measured synchronously with the intelligentized volum-densimeter, which was made. by ourself. These primary values of normal and diffuse pathologic changes can refer easily and used for the diagnosis in human autopsy of pathology and forensic pathology.

[Measuring volume and density of human organs and its significance in medicine and forensic medicine: lung].

Object: To obtain values of normal human lung and diffuse pathological changes, in order to provide a simple and convenient diagnostic method for measuring human visceral organs in the autopsy of pathology and forensic pathology.

Methods: The exact mass, volume and density of normal and pathological lung were synchronously measured with the intelligentized volume-densimeter made by authors.

Results: The date-base about the volume and density of human lung with different races were established for anatomy, anthropology and biodynamics.