Targeted Inhibitory Effect of Nasopharyngeal Carcinoma Cells by Hre.Grp78 Chimeric Promoter Regulating Fusion Gene .

Objective: To construct plasmids with Hre.Grp78 chimeric promoter regulating fusion gene and elaborate the effects of overexpressed on nasopharyngeal carcinoma cells.

Methods: Four plasmids were constructed, including pcDNA3.

Numb inhibits epithelial-mesenchymal transition via RBP-Jκ-dependent Notch1/PTEN/FAK signaling pathway in tongue cancer.

Background: Oral cancer has been estimated as the sixth most frequent solid cancer all over the world, in which tongue squamous cell carcinoma (TSCC) is the most common type of oral cancers. However, the mechanism of TSCC metastasizing to lymph node and distant sites has not been completely understood.

Methods: In this study, RT-qPCR method was used to detect the mRNA level of Numb, PTEN and Notch1 genes, as well as EMT-associated genes.

Association between RASSF1A Promoter Hypermethylation and Oncogenic HPV Infection Status in Invasive Cervical Cancer: a Meta-analysis.

Cervical carcinoma is the main cause of cancer-related mortality in women and is correlated with more than 15 risk cofactors, including infection of cervical cells with high-risk types of HPV (hrHPV). Indeed, both aberrant methylation of the RASSF1A promoter and hrHPV infection are often observed in cervical carcinomas. The purpose of our meta-analysis was to evaluate the role of RASSF1A promoter methylation and hrHPV infection in cervical cancer.

[Surgical management for residual or recurrent diseases in the neck after radiotherapy in nasopharyngeal carcinoma].

Objective: To investigate the surgical managements for residual or recurrent diseases in the neck after radiotherapy in nasopharyngeal carcinoma.

Methods: Seventy-eight cases of neck masses (39 cases for unilateral residual diseases, 9 for bilateral residual diseases and 30 for recurrent diseases) after radiotherapy in nasopharyngeal carcinoma who were treated surgically between January 1990 and December 2005 were retrospectively analyzed. There were 56 males and 22 females.

[Management of thyroid goiters invading mediastinum and thoracic cavity].

Objective: To investigate the diagnosis and treatments of thyroid goiters invading mediastinum and thoracic cavity.

Methods: Seventy-eight cases of thyroid goiters invading mediastinum and thoracic cavity and undergoing surgery from 1995 to 2005 were reviewed. There were 22 males and 56 females and their age ranged from 45 years to 78 years with a median age of 59 years.

The quorum-sensing system AvsR-AvsI regulates both long-chain and short-chain acyl-homoserine lactones in Agrobacterium vitis E26.

Agrobacterium vitis strain E26 is a nonpathogenic bacterium isolated from grape crown gall. In this study, the identification of a luxR-luxI type quorum-sensing system in strain E26 is reported. This system is involved in the induction of hypersensitive response on tobacco, but not its biocontrol activity.

[Targeted inhibition of vascular endothelial growth factor (VEGF) expression in human osteosarcoma cell line by antisense VEGF165 cDNA promoted by hypoxia reaction element].

Objective: Utilizing the hypoxia inducible factor 1/hypoxia reaction element (HIF-1/ HRE) gene regulation system to construct antisense vascular endothelial growth factor (VEGF165) cDNA eukaryotic expression vector promoted by HRE, and investigate its targeted inhibiting VEGF expression of osteosarcoma cells in hypoxia environment.

Methods: Eukaryotic expression plasmid with HRE promoter was constructed containing luciferase reporter gene and antisense VEGF165 cDNA by using PCR and recombinant DNA techniques. The recombinant vectors were transfected into osteosarcoma cells with lipofectin method.

Expression and hypermethylation of p27 kip1 in hepatocarcinogenesis.

Aim: To study the expressions of p27 kip1 protein and p27mRNA, the hypermethylation of p27 kip1 and the relation between them in various stages of hepatocarcinogenesis.

Methods: p27 protein and p27mRNA were detected by immunohistochemical staining and in situ hybridization respectively in 68 cases of normal liver, liver cirrhosis, pericancerous cirrhosis and hepatocellular carcinoma (HCC). The hypermethylation of p27 kip1 was detected by methylation-specific PCR (MSP) in 44 cases of normal liver, liver cirrhosis, and HCC.

[Expression and clinical significance of Fas-FasL of pleomorphic adenoma and adenoid cystic carcinoma in parotid gland].

Objective: To determine the expression and clinical significance of Fas and FasL in cases of occurrence and development of pleomorphic adenoma and adenoid cystic carcinoma(ACC) in parotid gland.

Methods: Using Immunohistochemistry techniques and quantitative analysis under computerized system, we investigated Fas-FasL expression in 21 cases of normal parotid gland, 35 cases of pleomorphic adenoma in parotid gland and 32 cases of ACC in parotid gland.

Results: All cases of ACC of parotid gland showed Fas and FasL negative expression, no correlation was found between the expression of Fas-FasL and the histological type and the pathological grades of ACC.

Detection of HBV, PCNA and GST-pi in hepatocellular carcinoma and chronic liver diseases.

Aim: To investigate the change of HBV DNA, PCNA and GST-pi in chronic liver disease and hepatocellular carcinoma (HCC).

Methods: Hepatitis B surface antigen (HBsAg), proliferating cell nuclear antigen (PCNA) and glutathione S-transferases (GST-pi) were detected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 111 specimens of chronic hepatitis, liver cirrhosis, paratumorous tissue, HCC and normal liver tissue.

Results: The positive rates of HBsAg and HBVDNA were 62.