Publications by authors named "Jin-yu Dong"
[Prokaryotic expression and purification of a tandem repeat of ovarian cancer antigen CA125 and preparation of its antiserum].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2012
Article Synopsis
- The aim of the study was to create a prokaryotic expression system for the CA125 tandem repeat protein (CA125R), purify the protein, and develop an antiserum against it.
- Researchers synthesized the CA125 gene, cloned it into a vector, transformed it into E.coli, optimized expression conditions, and used chromatography and Western blotting to purify and confirm the protein.
- The study successfully produced a high-purity CA125R protein and generated specific antiserum that recognizes both the recombinant and natural forms of the CA125 glycoprotein.