Clinical Prediction Nomograms to Assess Overall Survival and Disease-Specific Survival of Patients with Salivary Gland Adenoid Cystic Carcinoma.

Aim: Salivary gland adenoid cystic carcinoma (SACC) is the second highest incidence of malignant salivary gland tumor. The purpose of this study was to establish nomograms combined with SACC patients based on the Surveillance, Epidemiology, and End Results (SEER) database.

Methods: Patients with SACC were included in the SEER∗Stat Database from 2004 to 2016.

New Quorum Sensing System Inhibitor as an Antibacterial Synergist against Multidrug-Resistant .

Development of new bacterial biofilm inhibitors as antibacterial synergists is an effective strategy to solve the resistance of . In this paper, a series of 3-hydroxy-pyridin-4(1)-ones were synthesized and evaluated, and the hit compound () was identified with the effects of inhibiting the production of pyocyanin (IC = 8.6 μM) and biofilm formation (IC = 4.

Novel 2-Substituted 3-Hydroxy-1,6-dimethylpyridin-4(1)-ones as Dual-Acting Biofilm Inhibitors of .

2-Heptyl-3-hydroxy-4(1)-quinolone (PQS), a compound from , functions as both a quorum sensing (QS) regulator and a potent iron chelator to induce expression of pyoverdine and pyochelin which are involved in high-affinity iron transport systems. A potential dual-acting antibiofilm strategy requires molecules designed to interfere with iron uptake and the QS system of . A series of 2-substituted 3-hydroxy-1,6-dimethylpyridin-4-ones have been designed, synthesized, and tested as biofilm inhibitors of One compound, -((1,3,6-trimethyl-4-oxo-1,4-dihydropyridin-2-yl)methyl)hexanamide (), exhibits 68.

Application of CAD/CAM-assisted technique with surgical treatment in reconstruction of the mandible.

Objective: The purpose of this clinical study was to explore the optimal method of reconstruct mandible defects individually and immediately.

Study Design: Three-dimensional model simulation technique and vascularized fibular osteomyocutaneous flap were used to repair 15 cases of mandible defects, which were caused by ameloblastoma. A three-dimensional computed tomography (CT) images were converted to a virtual model using CAD software and the 3-dimensional (3D) simulated resin models of skeleton and fibula were used to design the osteotomies, bone segment replacement and titanium mesh shaping preoperatively.

Vitamin D Receptor Gene TaqI, BsmI and FokI Polymorphisms in Korean Patients with Tuberculosis.

Background: The active metabolite (1, 25-dihydroxycholecalciferol) of vitamin D (25-hydroxycholecalciferol) leads to activation of macrophages and deficiency of vitamin D seems to be involved in the risk of tuberculosis. The effects of vitamin D are exerted by interaction with the vitamin D receptor (VDR) and may be influenced by polymorphism in the VDR gene. In this study, variation in the VDR gene was investigated in Korean population with tuberculosis.

Comparison of two mice strains, A/J and C57BL/6, in caspase-1 activity and IL-1beta secretion of macrophage to Mycobacterium leprae infection.

A/J mice were found to have amino acid differences in Naip5, one of the NOD-like receptors (NLRs) involved in the cytosolic recognition of pathogen-associated molecular patterns and one of the adaptor proteins for caspase-1 activation. This defect was associated with a susceptibility to Legionella infection, suggesting an important role for Naip5 in the immune response also to other intracellular pathogens, such as Mycobacterium leprae. In this study, the immune responses of macrophages from A/J mice against M.

[Reconstruction of the tongue with rectus abdominis musculoperitoneal flap].

Objective: To investigate the outcome of surgical reconstruction of the tongue after hemiglossectomy with reinnervated rectus abdominis musculoperitoneal flaps in the treatment of tongue cancer.

Methods: Five patients underwent immediate reconstruction of the tongue and oral floor defects with rectus abdominis musculoperitoneal flaps after resection of squamous cell carcinoma of tongue. The rectus abdominis musculoperitoneal flap consists of the rectus muscle, posterior rectus sheath, peritoneum, the 10 th, 11th, 12th intercostal nerves and the vascular pedicle that includes the deep inferior epigastric artery and veins.

[Maxilla reconstruction with the free iliac osteomuscular flap and simultaneous osseointegrated implant embeding].

Objective: To evaluate the clinical outcome of reconstruction of maxillary defects with vascularized iliac crest flap and simultaneous osseointegrated implant embedding.

Methods: During September to October 2003, two patients with maxillary defects from tumor resection underwent microsurgical reconstruction. The free iliac osteomuscular flap transferring and simultaneous osseointegrated implant embedding were performed to repair the defects.

[The inhibitory effect of human endostatin gene on tumor growth of tongue squamous cell carcinoma].

Objective: To investigate the expression of transfected human endostatin (hES) gene in tongue squamous cell carcinoma (TSCC) and its inhibitory effects on the growth of tumor cells in vivo.

Methods: Lipofectamine-mediated hES gene was transferred into Tca8113 cells, selected with Blasticidin S; The stable transfected cells were inoculated in BALB/c mice, and then the growth of xenografts was observed. The hES and vascular endothelial growth factor (VEGF) protein expression of xenografts was detected by S-P immuno-histochemical assay.

[Study on the relationship between Fas expression and apoptosis in oral squamous cell carcinoma].

Objective: To investigate the relationship of Fas mRNA and protein expression and apoptosis in human oral squamous cell carcinoma.

Methods: Northern blot and flow cytometry (TUNEL method) were used to detect the expression of Fas mRNA and Fas protein, cell cycle and apoptotic level in oral squamous cell carcinoma. The relationship between Fas gene expression and OSCC apoptosis was analyzed statistically.

[Effect of fas gene transfection on tumourigenicity and proliferation of transplanted tumor of oral squamous cell carcinoma cell line Tca8113].

Objective: To investigate the effect of fas gene transfection and monoclonal anti-fas antibody on tumorigenicity and proliferation of transplanted tumor of Tca8113 cell.

Methods: Plasmid including fas gene was transfected into Tca8113 cell by lipofectamine kit. Some transfected cells were treated by monoclonal anti-fas antibody after 48 hours since transfection.

Tissue-specific down-regulation of RIPK 2 in Mycobacterium leprae-infected nu/nu mice.

RIPK 2 is adapter molecule in the signal pathway involved in Toll-like receptors. However, there has been no reported association between receptor-interacting serine/threonine kinase 2 (RIPK 2) expression and the infectious diseases involving mycobacterial infection. This study found that its expression was down-regulated in the footpads and skin but was up-regulated in the liver of Mycobacterium leprae-infected nu/nu mice compared with those of the M.

[Transfection of human endostatin gene with lipofectamin and the expression of hES protein in Tca8113 cell].

Objective: The purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.

Methods: To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.

[Relationship of apoptosis and expression of Fas gene in tongue squamous cell carcinoma cell line Tca8113].

Objective: To investigate the relationship of apoptosis and expression of Fas gene in oral squamous cell carcinoma.

Methods: Plasmid including Fas gene was transfected into Tca8113 cell by lipofectamine kit. The expression of Fas mRNA in transfected and non-transfected cell was detected by RT-PCR.

Missense mutations of the interleukin-12 receptor beta 1(IL12RB1) and interferon-gamma receptor 1 (IFNGR1) genes are not associated with susceptibility to lepromatous leprosy in Korea.

Interleukin-12 receptor beta 1 ( IL12RB1), interleukin-12 receptor beta 2 ( IL12RB2), and interferon gamma receptor 1 ( IFNGR1) perform important roles in the host defense against intracellular pathogens such as Mycobacteria. Several mutations within their genes have been confirmed as associated with increased susceptibility to mycobacterial infection. However, the association between mutations of the IL12RB1, IL12RB2, and IFNGR1 encoding genes and lepromatous leprosy has not been studied.

[Effect of c-myc transfection on fas expression and apoptosis in oral squamous cell carcinoma cell line Tca8113].

Background And Objective: The process of carcinogenesis is not only correlated to the abnormality of cell proliferation but also to the change of normal cell apoptosis. The aim of this study was to investigate the mechanism of apoptosis induced by c-myc(cellular avian myelocytoma virus) gene in oral squamous cell carcinoma cell line Tca8113.

Methods: Plasmid including c-myc gene was transfected into Tca8113 cell by using lipofectamine kit.