Effect of 's five-needle therapy pretreatment on airway inflammatory response in asthmatic rats based on ROS/TXNIP/NLRP3 pathway.

Objectives: To explore the possible mechanism of 's five-needle therapy pretreatment on relieving airway inflammatory response in asthmatic rats.

Methods: Forty SPF-grade SD rats were randomly divided into a blank group, a model group, an acupuncture group, and a medication group, with 10 rats in each group. Except the blank group, asthma model was established by aerosol inhalation of ovalbumin in the other 3 groups.

[Effect of acupuncture preconditioning combined with PI3K blocker LY294002 on airway inflammation in asthmatic rats].

Objective: To observe the effect of acupuncture preconditioning combined with PI3K blocker LY294002 on the expression of PI3K and Akt proteins and genes in the lung tissue and the contents of serum IL-12 and IL-13 in asthmatic rats, so as to explore its preprotective mechanism underlying improving asthma.

Methods: Sixty male Wistar rats were randomly divided into blank control, model, acupuncture pretreatment + blank, acupuncture pretreatment, acupuncture pretreatment + LY294002 and LY294002 groups (=10 in each group). The asthma model was established by intraperitoneal injection of mixture solution of OVA and Al(OH)3 and followed inhalation of 1%OVA for 30 min, once daily for 7 days.

[Professor 's clinical experience of fire needling for surgical diseases].

Professor -'s clinical experience of fire needling for bone-joint tuberculosis, tuberculous cervical lymphadenitis, ganglion cyst and thyrophyma is summarized. Professor used fire needling to treat bone-joint tuberculosis. The acupoints included points and nearby acupoints, particularly local opposite acupoints (Neixiyan [EX-LE 4] and Dubi [ST 35], Yinlingquan [SP 9] and Yanglingquan [GB 34], Xuehai [SP 10] and Liangqiu [ST 34]), and for the patients with severe -cold syndrome, decoction was additionally used.

[Effect of acupuncture and moxibustion at "Feishu""Dazhui"and "Fengmen"on the expression of CC chemokine ligand 1 and CC chemokine receptor 8 in lung tissue of rats with asthma].

Objective: To investigate the protein expression of CC chemokine ligand 1 (CCL1) and CC chemokine receptor 8 (CCR8) in the lung tissue of rats and the mechanism of acupuncture and moxibustion at "Feishu"(BL13), "Dazhui" (GV14) and "Fengmen"(BL12) in the treatment of asthma.

Methods: Sprague-Dawley rats were randomly divided into blank， model, acupuncture and moxibustion groups，=10 in each group. Ovalbumin sensitization via intraperitoneal injection was performed to establish a model of asthma.

["'s five-needle method" as the main treatment for allergic rhinitis and asthma syndrome: a multi-center randomized controlled trial].

Objective: To compare the clinical effect differences between "'s five-needle method" and routine acupoint selection on allergic rhinitis and asthma syndrome.

Methods: A total of 210 patients with allergic rhinitis and asthma syndrome were randomly divided into an observation group (105 cases, 4 cases dropped off) and a control group (105 cases, 4 cases dropped off). The patients in the observation group were treated with "'s five-needling method", and the acupoints of Feishu (BL 13), Dazhui (GV 14), Fengmen (BL 12), Yintang (GV 29), Shangyingxiang (EX-HN 8) and Hegu (LI 4), etc.

[Effect of acupuncture intervention on airway remodeling and transforming growth factor-beta1 expression in asthma rats].

Objective: To observe the effect of acupuncture stimulation of "Dazhui" (GV 14), bilateral "Fengmen" (BL 12) and "Feishu" (BL 13) on the thickness of the bronchial tubal wall and smooth muscle and expression of transforming growth factor-beta1 (TGF-beta1) in asthma rats so as to reveal its mechanism underlying improvement of asthma.

Methods: Forty SD rats were randomly assigned to control, model, acupuncture and medication groups. The asthma model was induced by subcutaneous injection of 1% egg albumin solution and forced inhalation of atomized ovalbumin.

Effects of moxibustion pretreating on SOD and MDA in the rat of global brain ischemia.

Objective: To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect.

Methods: The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male rats were randomly divided into 5 groups: a normal control group, a sham-operation group, a brain ischemia group, a brain ischemia preconditioning group, a moxibustion pretreating group.

[Talking about "moxibustion treatment for before sick" from "ischemic preconditioning"].

"Treatment for before sick" is a theory of TCM, reflecting preventing thought of "prevention being better than cure" and "nipping in the bud", while "moxibustion treatment for before sick" is highly praised by doctors of past ages. Moxibustion can activate human vital-qi and increase immunologic function of the organism, playing a preventive role for before sick, which is similar to the essence of "ischemic preconditioning" raised in recent years. Because of convenient manipulation, no adverse effect, it has very important position in the field of preventive medicine.

[Effects of moxibustion preconditioning on SOD and MDA in rats with global brain ischemia].

Objective: To reveal the mechanism of moxibustion preconditioning in preventive brain-protecting action.

Methods: The rat model of global brain ischemia was made with 4-artery ligation method. 78 Wistar rats were randomly divided into 5 groups: normal control group, sham-operation group, brain ischemia group, brain ischemia preconditioning group and moxibustion preconditioning group.