Geographic patterns of (Coleoptera: Lucanidae) species diversity and environmental determinants in China.

Clarifying the geographic patterns of species diversity and the determinant factors can provide essential information for species conservation and management. Stag beetles (Coleoptera: Lucanidae) of are important saproxylic insects and can be used for biomonitoring forests. Most of species are facing conservation concerns due to their limited distribution and fragmented habitats, particularly in China, which has the richest species diversity of this genus.

[Using extraction of red edge position to validate consistency of hyperspectral imaging and non-imaging data].

Using Pushbroom imaging spectrometer (PIS) and FieldSpec ProFR2500 (ASD), spectral reflectances of winter wheat and maize at different stages were collected synchronously. In order to validate the reliability of imaging spectral data, the red edge position of hyperspectral data for PIS and ASD were extracted by different algorithms, respectively. The following results were obtained: (1) The original spectrum of both instruments had high inosculation in red light region (670-740 nm); (2) With the spectra collected under laboratory condition (maize leaf), the extracted red edge position was is concentrated between 700 and 720 nm for the two instruments; (3) With the spectra collected undre field condition (wheat leaf), the extracted red edge position for PIS and ASD were different, the red edge position of PIS data was in 760 nm, while it was in 720 nm for ASD data.

[Investigation of the hyperspectral image characteristics of wheat leaves under different stress].

The diagnosis of growing status and vigor of crops under various stresses is an important step in precision agriculture. Hyperspectral imaging technology has the advantage of providing both spectral and spatial information simultaneously, and has become a research hot spot. In the present study, auto-development of the pushbroom imaging spectrometer (PIS) was utilized to collect hyperspectral images of wheat leaves which suffer from shortage of nutrient, pest and disease stress.

[Study on genetic polymorphisms of 13 SNP loci in Guangxi Zhuang population of China].

Objective: The fluorescence labeled multi-PCR system was applied to investigate the allele frequency of the 13 single nucleotide polymorphism (SNP) in 314 Guangxi Zhuang populations, and to evaluate their application value in forensic medicine.

Methods: Thirteen autosomal diallelic SNP loci were selected and the SNP genotyping system of fragment length discrepant allele specific fluorescence labeled multi-PCR technique was applied to investigate their allele frequency distribution in Guangxi Zhuang population.

Results: The allele frequencies of the 13 single nucleotide pdymorphism (SNP) in Guangxi Zhuang population were obtained, which shows that the allele frequency distribution is in accordance with Hardy-Weinberg equilibrium.

[A comparative study on genetic polymorphism and genetic relationship of 13 SNPs in three Chinese populations].

Using the fluorescence labeled capillary electrophoresis of multi-PCR technique, the single nucleotide polymorphism (SNP) typing system of fragment length discrepant allele specific fluorescence labeled multi-PCR technique is established based on the principle of allele-specific PCR. The typing of the 13 SNP loci can be completed simultaneously according to the length of PCR products and the number of product peaks. It appears a single product peak when the SNP is homozygous, and two product peaks with 4 bp differences will appear when it is heterozygous.

[A new method for SNP typing based on allele specific PCR].

Objective: To establish a new method of SNP typing.

Methods: Based on the principle of allele specific PCR and capillary electrophoresis technique, 11 diallelic SNP loci were selected and two forward primers with different length were designed for each SNP, with their 3' ends matched to the two alleles, respectively. An artificially mismatched base was also introduced into the third or fourth base in the 3' end area of the two forward primers in order to enhance the specificity of amplification.