Responsive regularity of neurons in the hindlimb region of primary somatosensory cortex to different temperature thermal needle stimulation of "Zusanli"(ST36) in mice.

Objectives: To study the regularity of central response to thermal needle stimulation of "Zusanli" (ST36) at different temperature, and to analyze the temperature difference of central responses.

Methods: Six male C57BL/6j adult mice were used in the present study. For observing activities of neurons in the hindlimb region of left primary somatosensory cortex (S1HL, A/P=0.

[Analysis on developmental characteristics of acupoint theory].

The theory of acupoints has gone through a long process from its embryonic form to its maturity, including its gradual improvement in later generations. Starting from the acupuncture literature in different historical periods, we, in the present paper, gave some examples and analyzed the connotation of important concepts in the development of acupoint theory. It is believed that the signs of the establishment of acupoint theory mainly include the standardization of acupoints' names, the specific description of acupoint positioning, the enrichment and explicitation of the content of acupoints' indications, the highlights of the meridian theory via classification of acupoints, and the interpretation of connotations of acupoints from a clinical perspective.

[Parameters and connotations of quantity of mild moxibustion stimulation].

The quantity of mild moxibustion stimulation is an important factor affecting clinical therapeutic effect. In the present study, we collected related literature by using key word "moxibustion stimulation quantity" from CNKI Database and make a summary about its concept. The quantity of mild moxibustion stimulation has two common characters, namely thermal intensity and cumulative stimulating quantity, and contains 6 components (parameters), including thermal energy, thermal stimulus, heated area of the skin, and stimulating duration, intensity and frequency.

[Could Jiaji (EX-B 2) Acupoint Be Assigned to the Governor Vessel ?].

The meridian tropism of acupoints is an important academic way to recognize and deal with the relationship between acupoints and meridians in acupuncture medicine. The Huatuo Jiaji (EX-B 2) (Jiaji for short) acupoint family consisting of 34 points has long been considered to be an important extra-acupoint since the ancient times of China and is widely employed in clinical practice through continuous development in Chinese past generations. The present paper introduces 1) historical origin and development of EX-B 2, and 2) intercorrelation among the EX-B 2, spine and the Governor Vessel in anatomical positions.

[Discussion on Classical Morphological View of Acupoints: Taking Book as An Example].

After analyzing the contents of , we propose a concept of classical morphological view about acupoints in terms of their superficial signs and structure in Chinese ancient times. That is to say that the ancient medical experts' understanding about acupoints contain locality, layers and variability. These 3 aspects were applied to the classification and location of acupoints, as well as to the clinical treatment in the ancient times.

Biomonitoring heavy metal contaminations by moss visible parameters.

Traditional sampling for heavy metal monitoring is a time-consuming and inconvenient method, which also does not indicate contaminants non-invasively and instantaneously. Moss is sensitive to heavy metals and is therefore considered a pollution indicator. However, it is unknown what kind physiological parameters can indicate metal contaminations quickly and non-invasively.

[An etiological survey on a foodborne disease epidemic outbreak caused by Salmonella enteritidis].

Objective: To conduct an etiological molecular epidemiological survey and laboratory test on a foodborne disease epidemic outbreak to make clear of the cause and implement effective prevention and control on it.

Methods: On May 12th 2012, 135 kindergarten children were sent to Xuzhou City People's Hospital and Children's Hospital with gastrointestinal infection disease. A total of 34 anus swab samples and 4 vomit samples were collected from the patients.

[Analysis on pathogen detection for 53 influenza outbreaks].

Objective: In order to provide a scientific basis for influenza prevention and control, analyzing the epidemic characteristics and laws of influenza outbreaks in Xuzhou area during 2005-2011.

Method: Using fluorescent-PCR method to detect influenza virus nucleic acid on Nasopharyngeal swab specimens collected from influenza outbreak cases during 2005-2011 and fast classifying influenza virus A1 (H1N1), A3 (H3N2), new H1N1 BV (Victoria) and BY (Yamagate) on subtypes. At the same time, isolating the influenza virus with MDCK cells, and sending them to the National Influenza Center for review, after the preliminary identification of the isolated influenza virus.

[Etiological analysis of influenza surveillance data in Xuzhou from 2005 to 2011].

Objective: To investigate the prevalence and subtypes of influenza viruses in Xuzhou city from 2005 to 2011 and to provide the scientific supports for influenza prevention and control in this religion.

Methods: The throat swab samples were collected from the influenza-like cases from national influenza like illness sentinel hospital in Xuzhou. The samples were used for influenza virus isolation and identification, sent on the national flu center to confirm according to the "national influenza surveillance program" and "influenza virus and experimental technology".

[Research on the aetiology of suspected enterovirus infected patients in Xuzhou district in 2009].

Objective: To understand and master the situation in which enterovirus caused hand-foot-and-mouth disease (HFMD) in Xuzhou district in 2009 so as to provide scientific basis for the control and prevention of hand-foot-and-mouth disease.

Methods: The researchers adopted fluorescence RT-PCR method to detect EV and EV71 as well as the CA16 specificity RNA from 222 samples of anal swabs and oropharyngeal swabs from the 240 cases who were diagnosed clinically as hand-foot-mouth disease infected by enterovirus. Also, the researchers conducted EV71-IgM antibody detection on 114 samples of acute phase serum with ELA method.

[Molecular epidemiology of enterohaemorrhagic Esacherichia coli O157 in some areas in China].

Objective: To understand the epidemiological characteristics of enterohaemorrhagic Escherichia coli (EHEC) O157 and to determine the degree of its genetic relations.

Methods: Polymerase chain reaction (PCR) techniques and chromosomal DNA digested by restriction enzyme Xba I according to PulseNet directions by pulsed field gel electrophoresis (PFGE) method were applied to 300 E. coli O157 strains isolated from patients and animal sources from 1988 to 2005 from Henan, Jiangsu and Anhui provinces.

[Molecular typing of the pathogenic Yersinia enterocolitica strains with pulsed field gel electrophores isolated in China].

Objective: To investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China,using pulsed field gel electrophoresis(PFGE) and standardized PFGE method as well as typing database of Yersinia enterocolitica.

Methods: PFGE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0, Applied Maths BVBA, Belium).

[Epidemiological study on an outbreak caused by E. coli O157:H7 in Jiangsu province].

Objective: To carry out epidemiological study on an outbreak caused by E. coli O157:H7 infection in Jiangsu province in 1999.

Methods: Epidemiological, microbiological and moleculebiological methods were used to find out the source, route of transmission and risk factors.

[Escherichia coli O157:H7 strains carrying Shiga toxin 2 variant slt2vha were isolated from Xuzhou City of Jiangsu Province].

Objective: To monitor the changes of predominant Shiga toxin types of Escherichia coli O157:H7 in Xuzhou City, Jiangsu Province.

Methods: PCR typing with slt2-specific primer pairs (slt2cslt2, slt2v1slt2v2), hybridization of chromosome DNA digested by PstI, and DNA sequencing of PCR products. with slt2-specific primer pairs (slt2cslt2d; slt2eslt2f) were conducted on 14 strains of E.