Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF-κB Signaling Pathway.

Objective: To investigate the changes in proinflammatory cytokines and chemokines, namely, C-C motif ligand (CCL) 2 and CCL7, in postmenopausal osteoporosis (PMOP) and to develop a new drug, bindarit (Bnd), for PMOP in an ovariectomized (OVX) mouse model.

Methods: Bone marrow macrophages (BMMs) from the femurs of five women with PMOP and five premenopausal women without osteoporosis were detected by RNA sequencing. BMMs from mice were differentiated into osteoclasts and treated with a synthetic inhibitor of CCL2 and CCL7, Bnd, or 17 beta estradiol (E ).

Relationship between the laminectomy extension and spinal cord injury caused by acute spinal shortening: goat in vivo experiment.

Objective: To investigate the relationship between different types of laminectomy extension and spinal cord injury subsequent to acute spinal shorting after 3-column osteotomy in living goat model.

Methods: A total of 18 healthy goats were selected, and a procedure of bivertebral column resections and total laminectomy of T13 and L1 was completed followed by different laminectomy extensions under the somatosensory evoked potential (SSEP) monitoring. The samples were divided into three groups according to types of subsequent laminectomy extension.

Serum cystatin C is increased in acute spinal cord injury: a multicentre retrospective study.

Study Design: A multicentre retrospective study.

Objective: A multicentre retrospective study was performed to observe the changes in serum cystatin C (CysC) levels in patients with acute spinal cord injury (SCI).

Setting: Four hospitals in China.

[Advances in prostate-specific membrane antigen-mediated antitumor drugs].

Prostate cancer is the most prevalent male malignancy in the United States, and remains the second leading cause of cancer-related death in the male population. Prostate specific membrane antigen(PSMA) is a type of II transmembrane glycoproteins that is over-expressed in prostate cancer cell. More importantly, its expression is increased with cancer progression.

Glycemic Variation in Tumor Patients with Total Parenteral Nutrition.

Background: Hyperglycemia is associated with poor clinical outcomes and mortality in several patients. However, studies evaluating hyperglycemia variation in tumor patients receiving total parenteral nutrition (TPN) are scarce. The aim of this study was to assess the relationship between glycemia and tumor kinds with TPN by monitoring glycemic variation in tumor patients.

Influence of vertebral column distraction on spinal cord volume: an experimental study in a goat model.

Introduction: Spinal cord injury may be related to excessive distraction of the spinal cord during surgical correction of spinal deformities by vertebral column resection. This study aimed to investigate how vertebral column distraction influences spinal cord volume to establish the safe range in a goat model.

Materials And Methods: A vertebral column resection was performed on the tenth thoracic vertebra of 11 goats.

Relationship between Spinal Cord Volume and Spinal Cord Injury due to Spinal Shortening.

Vertebral column resection is associated with a risk of spinal cord injury. In the present study, using a goat model, we aimed to investigate the relationship between changes in spinal cord volume and spinal cord injury due to spinal shortening, and to quantify the spinal cord volume per 1-mm height in order to clarify a safe limit for shortening. Vertebral column resection was performed at T10 in 10 goats.

The application of atlantoaxial screw and rod fixation in revision operations for postoperative re-dislocation in children.

Introduction: We evaluate the feasibility, safety, and efficacy of atlantoaxial screw and rod fixation for revision operations in the treatment of re-dislocation after atlantoaxial operations in children.

Methods: Eight consecutive children with atlantoaxial instability required a revision operation due to atlantoaxial re-dislocation caused by the failure of the initial posterior wire fixation. The children were 5-11 years of age with an average age of 8.

Clinical application of computer-aided design-rapid prototyping in C1-C2 operation techniques for complex atlantoaxial instability.

Study Design: This is a retrospective clinical study.

Objective: To evaluate the clinical efficacy of computer-aided design-rapid prototyping (CAD-RP) techniques in surgical treatments for atlantoaxial instability (AAI).

Summary Of Background Data: The complexity of the upper cervical anatomic structures makes the procedures for the treatment of AAI particularly challenging for surgeons.

Enhancement of the synthesis of n-3 PUFAs in fat-1 transgenic mice inhibits mTORC1 signalling and delays surgically induced osteoarthritis in comparison with wild-type mice.

Background: An exogenous supplement of n-3 polyunsaturated fatty acids (PUFAs) has been reported to prevent osteoarthritis (OA) through undefined mechanisms.

Objective: This study investigated the effect of alterations in the composition of endogenous PUFAs on OA, and associations of PUFAs with mammalian target of rapamycin complex 1 (mTORC1) signalling, a critical autophagy pathway in fat-1 transgenic (TG) mice.

Methods: fat-1 TG and wild-type mice were used to create an OA model by resecting the medial meniscus.

New clinical classification system for atlantoaxial dislocation.

The purpose of this study was to define a new clinical classification of atlantoaxial dislocation based on its clinical manifestations, namely reducible atlantoaxial dislocation (RAAD), irreducible atlantoaxial dislocation (IAAD), and fixed atlantoaxial dislocation (FAAD). A total of 107 patients with atlantoaxial dislocation were respectively treated based on this clinical classification, including 66 patients with RAAD, 39 patients with IAAD, and 2 patients with FAAD. Six of the 66 patients with RAAD with rotatory atlantoaxial dislocation were treated with traction and a cervical collar, 9 with fresh type II dens fracture were treated with cannulated screw fixation, and 51 were treated with posterior atlantoaxial or occipitocervical arthrodesis.

γ-secretase inhibitor-I enhances radiosensitivity of glioblastoma cell lines by depleting CD133+ tumor cells.

Background And Aims: Glioblastoma is a deadly primary brain tumor with great resistance to radiotherapy. To reverse its radioresistance is important for improving prognosis. Gamma-secretase inhibitors (GSI) have been proven to have anti-tumor effects, yet the knowledge of their influences on glioblastomas is still limited.

Personalised modified osteotomy using computer-aided design-rapid prototyping to correct thoracic deformities.

The correction of severe thoracic deformities is challenging. However, the usual imaging modalities are not sufficient for performing the surgery. Our objective was to describe the procedure and results of posterior modified wedge osteotomy aided by the techniques of computer-aided design-rapid prototyping (CAD-RP) to correct thoracic deformities.

5-(4-Chloro-anilinomethyl-ene)-2,2-dimethyl-1,3-dioxane-4,6-dione.

The title compound, C(13)H(12)ClNO(4), is approximately planar, with a dihedral angle of 8.23 (4)° between the mean plane of the amino-methyl-ene unit and the planar part of the dioxane ring. The dioxane ring has a half-boat conformation, in which the C atom between the dioxane O atoms is -0.

1-(4-Hydr-oxy-3,5-dimethoxy-phen-yl)ethanone.

In the title mol-ecule, C(10)H(12)O(4), the non-H atoms are essentially coplanar (r.m.s.

5-(4-Bromo-anilinomethyl-ene)-2,2-dimethyl-1,3-dioxane-4,6-dione.

In the title compound, C(13)H(12)BrNO(4), the dihedral angles between the amino-methyl-ene group and the dioxane ring and between the benzyl ring and the amino-methyl-ene unit are 7.96 (4) and 12.15 (4)°, respectively.

[Preparation of cisplatin-impregnated coral hydroxyapatite drug delivery system].

Objective: To prepare a cisplatin-impregnated coral-derived hydroxyapatite (CCHA) drug delivery system (DDS), and evaluate its inhibitory effect on human osteosarcoma cells U-2 OS, human breast cancer and prostatic carcinoma cells PC-3 in vitro.

Methods: The coral-derived hydroxyapatite (CHA) was manufactured by hydrothermal exchange and impregnated with cisplatin by vacuum freeze-drying techniques. The leaching solutions of this DDS was collected at different intervals in a course of 8 weeks and their inhibitory effect on the cells was tested in vitro by MTT assay.

[Sensitization of human glioma SWO cell line to tumor necrosis factor-induced apoptosis by blocking phospholipase C-gamma1 signaling pathway].

Objective: To explore the role of phospholipase C-gamma1 (PLC-gamma1) in tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis of human glioma SWO cells.

Methods: The PLC-gamma1 pathway was blocked by U73122 in SWO cells, and the inhibitory effect of TNF-alpha on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay. The cell apoptosis induced by TNF-alpha along or in combination with U73122 was detected by flow cytometry with PI staining.

[Impact of early-stage hepatic ischemia-reperfusion injury on other organs of rats].

Objective: To investigate the influence of hepatic ischemia-reperfusion on other organs of rats during the early stage of injury.

Methods: Twenty-five healthy male SD rats were randomized equally into 5 groups according to the different time points in the course of hepatic vascular occlusion and reperfusion, namely pre-occlusion, the end of occlusion, and after 0.5, 2.

[Establishment of canine models of acute mesenteric ischamia].

Objective: To establish a new canine model of acute mesenteric ischemia for imaging studies.

Methods: Ten Beagle dogs were divided into ischemic group (n=8) and control group (n=2). Via a 4F Cobra catheter, the absolute ethanol was selectively injected into the superior mesenteric artery (SMA) for embolization in the former group while the saline was injected in the latter.

[Three-dimensional dynamic contrast-enhanced magnetic resonance angiography in liver transplantation].

Objective: To investigate the clinical value of three-dimensional dynamic contrast-enhanced magnetic resonance (MR) angiography in the liver transplantation.

Methods: MR scanning was performed both before and after the operation in 9 patients elected for liver transplantation, and after three-dimensional reconstruction at a computer workstation, the three- dimensional images of the inferior vena cava, hepatic veins, portal vein and bile duct were obtained.

Results: The three- dimensional images could be rotated in space to show the size, shape, lining of the veins and anatomic relationships between each other.

Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats.

Objective: To investigate the early-stage multiple organ injuries in rats subjected to intestinal and hepatic ischemia-reperfusion.

Methods: Seventy-five normal male Wistar rats were randomized equally into hepatic ischemia, intestinal ischemia and intestinal-hepatic ischemia groups. Before and at the end of occlusion (45 min), as well as at the time points of 0.

[Application of ischemic preconditioning before hepatic vascular exclusion for resection of hepatocellular carcinoma].

Objective: To assess the value of clinical application of ischemic preconditioning (IP) before hepatic vascular exclusion for resection of hepatocellular carcinoma (HCC) accompanied by cirrhosis and explore the possible mechanism underlying the protective effect of this maneuver.

Methods: Thirty-four consecutive patients with resectable HCC were randomized into IP group to receive IP with a 5-min ischemia followed by 5-min perfusion before hepatic vascular exclusion and the control group with simply hepatic vascular exclusion. The liver function, hepatic Fas mRNA expression, caspase-3 activity, apoptosis of the hepatocytes were compared between the two groups.

[Combined operations for patients with hepatic cancer complicated by portal hypertension].

Objective: To study the feasibility and operative procedures of liver resection in combination with collateral devascularization for treating patients with hepatic cancer complicated by portal hypertension.

Methods: A retrospective analysis was conducted in 45 cases of hepatectomy for hepatic cancer in combination with portaazygous devascularization for portal hypertension from Jan. 1995 to Jan.