A lysosome-targetable fluorescent probe for real-time imaging cysteine under oxidative stress in living cells.

As an effective lysosomal biomarker for oxidative stress status, cysteine (Cys) plays an important role in lysosomal proteolysis. Herein, we report the first lysosome-targetable fluorescence probe (MCAB) for Cys-selective detection based on nucleophilic addition reaction of sulfhydryl toward a α, β-unsaturated ketone and demonstrate its application to lysosomal-targetable imaging. MCAB is designed based on a α, β-unsaturated ethanoylcarbazole as the fluorophore and the thiols reaction site, and a methylcarbitol unit as a lysosome-targetable group.